Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"EMS mutagenesis"”.
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Characterization of a novel cellulose biosynthesis inhibitor, CBI28, in Gluconacetobacter xylinus
… an inhibitor along with classical genetics and EMS mutagenesis. An EZ-Link Biotin Hydrazide Kit was used to create a CBI28-Biotin conjugate for further studies. Gluconacetobacter xylinus cells were exposed to 10 uM CBI28 to induce cellulose biosythesis inhibition, lysed and small hydrophobic …
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The receptor tyrosine phosphatase Ptp69D and the receptor tyrosine kinase Pvr in Drosophila nervous system development
… dominant-negative phenotype. In mammalian systems, Src plays a key role in the regulation of cell adhesion, and Abl is a known regulator of actin cytoskeletal dynamics. Removing a single dose of the EGF receptor or Ras85D suppressed the Ptp69D dominant negative phenotype. Interestingly, the …
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Mutational analysis of JIL-1, a tandem kinase in Drosophila
… the limitation of deletion/insertion mutations, EMS mutagenesis was performed to induce point mutations of JIL-1 and the mutation screen was designed to recover temperature-sensitive JIL-1 alleles. 11 new mutant alleles were identified and 4 were selected for further analysis. The mutant …
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A forward genetic screen to identify factors that control meiotic recombination in Arabidopsis thaliana
… formation have been identified in model systems. For example, SPO11 mediates generation of meiotic DNA double-strand breaks (DSBs) across all eukaryotes, which may be repaired as crossovers. However, downstream regulators of recombination remain to be identified, including those with …
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Regulation of synaptic structure and function at the Drosophila neuromuscular junction
… of synapse assembly, I carried out a large-scale EMS mutagenesis screen of the second chromosome. From this screen I identified a mutation in actin 57B that disrupts synaptic morphology and presynaptic active zone organization. Actin 57B is one of six actin genes in Drosophila and is expressed in …
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The Pro-Apoptotic Function of CED-9 Requires a CED-9-CED-4 Interaction
… extrusion in the absence of apoptosis. From an EMS mutagenesis screen, performed on worms lacking caspase activity, searching for survival of an extruded cell (ABplpappap), I isolated a nonsense mutation in the GMP reductase gene, gmpr-1. Interestingly, this allele (n6457) also allows ABplpappap …
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Characterisation of mutants involved in Phytochrome A nuclear import and signal transduction
In the present work, an Ethyl Methane Sulphonate (EMS) mutagenesis screen was performed using a transgenic line expressing phyA: GFP fusion protein. Aim of the screen was to isolate and characterise Phytochrome A (phyA) nuclear transport mutants. The screening was performed in two steps. First, the …
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Utilizing maize genomics for pre-breeding insights
… mutant obtained from ethyl methanesulfonate (EMS) mutagenesis, has not been previously mapped. Phenotypically, Yg3-N1582 has yellow color expression at coleoptile emergence that does not persist beyond the seedling stage, is homozygous-viable, and is non-lethal with no apparent deleterious …
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Exploring the Potential Use of Camelina Sativa as a Biofuel Crop for Nevada
… seeds were treated with ethyl methane sulfonate (EMS) and twenty-five randomly selected lines of an M3 generation EMS population were evaluated for fatty acid composition. Increased variation in fatty acid composition was observed when compared with the control, wildtype cultivar. Most …