Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"ELISA assay"”.
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Expression of HIV-1 subtype C nef in E. coli and Nicotiana benthamiana : development of plant-based vaccines for HIV
… tool in the development of an indirect ELISA assay for detection of Nef in Nicotiana benthamiana. An indirect ELISA assay was developed using a commercially available polyclonal anti Nef antiserum raised in sheep. The role of codon optimization in expression of Nef in benthamiana was …
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2D layout of bead based thioaptamer/aptamer selection platform for therapeutics and diagnostics
… layout of beads and its corresponding functional assays, a model system is first established: NF-kappa B proteins were expressed, purified and characterized. Thioaptamer XBY6, which specifically targets NF-kappa B protein, and its natural origin, I-kappa B were synthesized and verified. …
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Modulation of RANKL and Osteoprotegerin in Adolescents Using Orthodontic Forces
… RANKL and OPG expression was measured by ELISA assay. Expectations were that (1) force would raise RANKL and diminish OPG, (2) force removal would reverse the RANKL-OPG levels, (3) strength and duration of force are associated with RANKL-OPG levels, and (4) responses would exhibit …
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Development of Electrochemical Immunosensor for Oral Cancer: Initial Outcome
… stages of the disease. Additionally, an ELISA assay was performed to validate sensor performance and benchmark biomarker detection. The findings demonstrate consistent trends in biomarker expression and validate the potential of the sensor for early-stage diagnosis. This integrated …
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Detection of glial interleukin-6 release: Development of a novel interleukin-6 Elisa
… (IL-6) enzyme-linked immunosorbent (ELISA) assay resulted in a detection range of 1 ng/m1 to 10 ng/mL IL-6. The limit of detection (LOD) for IL-6 was 0.25 ng/mL. Troubleshooting techniques were attempted including matrix effects, non-specific binding, and coating buffer pH …
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Optimization of Downstream Purification of Lucentis
… obtained from each case were analyzed using ELISA Assay, electrophoresis, and EZQ to indicate the presence of a Lucentis like fAb-2 molecule. The base case and alternative cases were analyzed for product purity and yield and an economic analysis of all four processes were performed. Other …
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Novel hypothalamic G protein-coupled receptors involved in the control of reproduction
… epitope-tagging expression vector. A receptor ELISA assay and receptor signalling assay were utilized to compare wild-type (WT) and mutant receptor cell surface expression and functionality in vitro. 2/6 bioinformatic tools predicted the L282V mutation would be deleterious and 3/6 predicted …
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Plasma N-terminal Proatrial Natriuretic Peptide Concentration in Cats with Hypertrophic Cardiomyopathy
… cats. Plasma [Nt-proANP] was determined using an ELISA assay. The relationship between plasma [Nt-proANP] and M-mode, 2-dimensional and Doppler echocardiographic variables was evaluated. Cats that were hyperthyroid or had evidence of renal disease were excluded from the study. Results: The mean …
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ENHANCEMENT OF hFVIII ACTIVITY THROUGH LC MODIFICATIONS FOR GENE THERAPY OF HEMOPHILIA A
… fold increase in FVIII clotting activity. An ELISA assay revealed no significant difference between productions of the heavy or light chains at any time point. By comparing the clotting activity to the amount of protein produced, it was determined that the increase in coagulation activity was …
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Can curcuminoids be more effective than sulfasalazine for the treatment of rheumatoid arthritis?
… expression profile of the candidate genes. CRP ELISA assay was used to confirm the findings for IL-6 gene expression in untreated and treated conditions. Curcumin exhibited increased potency in downregulating gene expression in comparison to the other treatments. Therefore, the potential use of …
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INDUCED SPAWNING OF CAPTIVE TRIPLETAIL LOBOTES SURINAMENSIS
… of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this …
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Concentration of sulfamethazine in spray dried milk
… quantitatively by HPLC, a microbial receptor assay (Charm-II®) and an ELISA assay (LacTek®) and qualitatively by an ELISA method (Cite®) in milk samples before and after spray drying. Dry milk samples were reconstituted (10% w/w) for all analyses. statistical determination of significant …
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Histone Deacetylase 1: Mutagenesis And Small Molecule Studies
… N-SAHA analogs were screened using a plate based ELISA assay which we developed and validated. All SAHA analogs screened did not qualify for a HDAC1-bump inhibitor system.</p>
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The effect of antibiotic treatment on Haemophilus parasuis colonization, disease and immunity
… H. parasuis in swine. First, a species-specific ELISA assay and an experimental model of H. parasuis colonization were developed to study the immune response to H. parasuis colonization by a pathogenic strain in conventional pigs. Moreover, the effect of the antimicrobial enrofloxacin in reducing …
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Protection from HCV infection – Identification of mechanisms of resistance to HCV infection in exposed uninfected injection drug users.
… to low dose viral RNA in EU cases utilising ELISA assay. In conclusion, this thesis reports data that identifies a number of new findings that provide insight into mechanisms of resistance to HCV infection. My findings suggest that the complex interplay between the virus and lipids together …
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Catalytic antibodies for amide cleavage and prodrug activation
… supematants. A new method called "Capture"-CatELISA was developed, which allowed the efficient screening for catalysis of hundreds of hybridoma supematants in a very short period of time. To this end, a substrate Cl was developed, which upon activation covalently traps catalytic antibodies, and …
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Interactions of Francisella Tularensis With Components of the Host Fibrinolytic System
… fresh frozen human plasma. By using an ELISA assay I was able to detect significant levels of plasminogen and fibronectin binding to the bacterial cell surface. Based on this observation, further studies were designed to dissect possible <em>in vitro</em>and <em>in vivo</em> functions of …
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Regulation of Clathrin Mediated Endocytosis and Its Role in Cancer Progression
… in cancer. To test this, we developed In-Cell ELISA assays to measure three different endocytic pathways: clathrin-mediated endocytosis, caveolae-mediated endocytosis, and clathrin-independent endocytosis and compared these activities in 29 independently isolated non-small cell lung cancer …
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Detection of campyloabacter jejuni and campylobacter coli in high risk foods and environmental waters using the polymerase chain reaction
… colorimetric end-stage detection format (PCR ELISA). The sensitivity of detection of the PCR ELISA assay was equivalent to one genome copy and was demonstrated to be 100-fold more sensitive than a conventional gel electrophoresis-based PCR method. The novel species-specific PCR assay was …
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Neuroprotective and immunomodulatory properties of the new PPARγ agonist MDG548: an in vitro and in vivo study in PD models
… molecules were measured in the medium by ELISA assay and phagocytosis was evaluated by fluorescent immunohistochemistry for CD68. MDG548 arrested dopaminergic cells degeneration in the SNc in both the subacute MPTP and the chronic MPTPp models of PD, and reverted MPTPp-induced motor …
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