Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 194 for “"EGFP"”.
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Expression and characterization of plant produced AHSV nanoparticles encapsulating EGFP
… protein, enhanced green fluorescent protein (EGFP), to the VP3 structural protein of African horse sickness virus (AHSV). Plant-based transient expression of the AHSV EGFP-VP3 and AHSV VP7 structural proteins has been used to generate CLPs from N. benthamiana leaves that successfully …
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Lokalisation und Dynamik der Replikationsproteine des murinen prä-replikativen Komplexes
… die zu untersuchenden Replikationsproteine als EGFP-Fusionsproteine in LTK--Zellen exprimiert und am konfokalen Laserscanning-Mikroskop untersucht. CDC6-EGFP war in der G1-Phase diffus in Zellkern und Cytoplasma verteilt, am G1/S-Übergang ausschließlich im Zellkern lokalisiert und während der …
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Structural and Functional Study of Multidrug Membrane Transporters
… aeruginosa</em>), respectively. We fused EGFP with N-terminus and C-terminus of BmrA to construct BmrA-EGFP fusion proteins and characterized their efflux functions in four strains of bacterial cells (Ct-BmrA-EGFP, Nt-BmrA-EGFP, BmrA and ▵BmrA, B. <em>subtilis</em>) using fluorescence …
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Development of Tools to Assess the Effects of Lunasin on Normal Development and Tumor Progression in Drosophila Melanogaster
… in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. Progeny were assessed …
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Localization and dimerization of the ABC half transporter rAbcb6 as compared to rAbcb7
… Expressionsvektoren, die für rAbcb6-EGFP, rAbcb6-V5, rAbcb6-DsRed2, rAbcb7-EGFP, rAbcb7-V5 und rAbcb7-DsRed2 Fusionsproteine kodieren, wurden kloniert und bei der transienten Transfektion von Säugetierzellen verwendet.In transienten kotransfizierten menschlichen LoVo-Zellen wurde …
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A Novel Transgenic Rat Model for the Study of Germ Cell Biology
… the body, a serendipitous integration of a ROSA-EGFP transgene resulted in exclusive expression of EGFP in the germ cells of both sexes. EGFP expression was uniform and robust in cleavage stage embryos beginning at the late 2-cell stage and continuing through blastocyst development where …
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Creation and establishment of transgenic mouse models for for Mecp2 gene, causing Rett syndrome
… zu generieren, die Mecp2 gekoppelt an EGFP als Reporter exprimieren. Dadurch erhofften wir uns eine genauere Charakterisierung von Mecp2 und eine angemessene funktionelle Analyse durchführen zu können. Das transgene Konstrukt wurde durch GET Rekombination eines BAC-Klones (B22804) …
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Generation of Transgenic Mice to Evaluate Promoter Activity and Specificity of Two Human Endogenous Retrovirus Long Terminal Repeats = Untersuchungen zur Promotor-Aktivität und -Spezifität von zwei Long Terminal Repeats humaner endogener Retroviren in transgenen Mäusen
… into a enhanced green fluorescent protein (EGFP) expression vector. Transgenic mice were generated by DNAmicroinjection into pronuclei of zygotes. One pBL-HERV-L transgenic line and four pEGFP-HERV-H-H6 transgenic lines were established and analyzed. While the HERV-L promoter was not active …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… the gene of enhanced green fluorescence protein (EGFP) and then expressed in HeLa cells. Fluorescence images of the transfected cell showed that the AK1-EGFP was homogeneously distributed throughout the cell, while AK1beta-EGFP was mainly localized on the plasma membrane. The diffusion rates of …
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Assessment of Murine Embryo Development Following Electroporation and Microinjection of a Green Fluorescent Protein DNA Construct
… the prepared enhanced green fluorescent protein (EGFP) construct as a visual marker of protein expression was evaluated using pronuclear microinjection. Embryo development and fluorescence were evaluated following pronuclear injection of EGFP at a concentration of 3 μg/ml and compared to an …
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Investigating the maintenance of the mouse definitive adrenal cortex
… in vitro. In the present study an AS-mCherry-11B-EGFP BAC construct was successfully engineered, in which Cyp11b2 and Cyp11b1 were substituted by mCherry and EGFP, respectively. This BAC construct was characterized in mouse adrenocortical Y1 cells. It was determined that EGFP faithfully …
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Expression of Foreign Genes in the Pseudomonas Bacteriophage Pf3
… synthesize Enhanced Green Fluorescent Protein (EGFP). Type 88 phage display on the EGFP recombinant Pf3 was attempted by fusing a second copy of its MCP gene to the existing EGFP gene. This resulted in a phage display Pf3 replacement vector which was then used to construct a phage for displaying …
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Investigating the role of nuclear transport proteins secreted by cancer cells
… to enhanced Green Fluorescent Protein (Kpnb1-eGFP) containing secreted protein isolates obtained from cervical cancer cell lines on the biology of cell lines of cervical cancer and non-cancer origin. Conditioned media collected from control, eGFP-expressing or Kpnb1-eGFP expressing HeLa and …
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GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging
… and 100%) of enhanced green fluorescent protein (EGFP); and the total amount of EGFP bound per single microparticle was determined. EGFP- coated microparticles were detected and imaged by a standard epi-fluorescence inverted microscope combined withOlympus Scan^R automated image acquisition …
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Zwei-Hybrid-Fluoreszenzkreuzkorrelationsspektroskopie : In vivo Charakterisierung von Protein-Protein-Wechselwirkungen im AP-1-System
… können. Unter den getesteten AFPs (EYFP, ECFP, EGFP, DsRed2, mRFP1 und eqFP611) war das Chromophorpaar EGFP-mRFP1 das einzige, das zum Erfolg führte. Der FCCS-Aufbau wurde in vivo einerseits mit einem Fusionsprotein aus EGFP und mRFP1 als Referenz für 100 % Kreuzkorrelation und andererseits mit …
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Ανάπτυξη και εκτίμηση του δυναμικού επισωματικών και ιϊκών φορέων για την γονιδιακή μεταφορά σε κυτταρικά συστήματα και σε κλινικές δοκιμές στην γονιδιακή θεραπεία
… η EBV κατασκευάσαμε τα εξής πλασμίδια: 1) pEBS/eGFP με το S/MAR να είναι κλωνοποιημένο μετά το γονίδιο της eGFP όπως στο pEPI-1. 2) pESdER / eGFP προερχόμενο από το pEBS/eGFP με διαγραφή του γονιδίου EBNA-1 και.3) pCEP4 / eGFP (πλασμίδιο μάρτυρας). Σε αντίθεση με τις προσδοκίες μας μόνο το …
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Lamin C2
… eine ektopische Expression von Lamin C2 als EGFP-Lamin C2-Fusionsprotein in einer somatischen Zellinie zeigte sich, daß eine neuartige Hexapeptidsequenz (GNAEGR) am N-terminalen Ende des Proteins anstelle der C-terminal gelegenen CaaX-Box somatischer Lamine für die Interaktion mit der …
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