Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"EF-hand"”.
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Structural And Mechanistic Elucidation Of The EF-hand In LETM1 Ca2+/H+ Antiporter Function
… homeostasis is tightly regulated. Leucine zipper EF-hand containing transmembrane protein-1 (LETM1) is an essential inner-mitochondrial membrane protein that regulates Ca2+ through exchange for protons (H+). LETM1 is an essential gene where haploinsufficiency is associated with Wolf-Hirschhorn …
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Function of a Unique Dually Localized EF-Hand Domain Containing Protein, TgEFP1, During the Lytic Cycle of the Human Parasite Toxoplasma Gondii
… our lab has identified a novel protein-TgEFP1 (TGGT1_255660), which is predicted to bind Ca2+ through its two EF-hand domains. Interestingly, TgEFP1 showed a unique dual localization at the PLV/ELC and the PV of the parasite. Previous work showed that the PLV/ELC harbors other ion binding …
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Strukturbestimmung des Birkenpollenallergens Bet v 4
… kleinen Calcium bindenden Allergenen mit je 2 EF-Hand Strukturmotiven, die in einer Vielzahl höherer Pflanzen gefunden werden. Es wurden effektive Strategien zur Präparation von rekombinantem Bet v 4 entwickelt, die auch eine Markierung mit dem Isotop 15N erlaubten. Zwei hervorstechende …
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Characterization and molecular cloning of sos3: A gene important for salt tolerance and potassium nutrition in higher plants
… Ca²⁺ levels can partially suppress the growth defect of the mutant plants under salt stress and fully restore their growth under low K⁺. These results suggest that SOS3 may be a Ca²⁺-mediated regulator that controls K⁺ and Na+ homeostasis in Arabidopsis. The SOS3 gene was cloned by map-based …
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Characterization of the Protein Phosphatase 2A Regulatory Subunit PR70
… PR70 contains two conserved calcium binding EF hand motifs and binds calcium in vitro. Calcium enhances the binding of PR70 to the A-subunit but not to Cdc6. Although calcium did not enhance the binding of PR70 to Cdc6, it did result in an increase in the amount of PP2A associated with Cdc6. …
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Mitochondrial Calcium Uptake: LETM1 and MICU1 Are Mitochondrial Proteins That Regulate Mitochondrial Calcium Homeostasis and Cellular Bioenergetics
… mitochondrial Ca2+ uptake. Leucine Zipper EF hand containing Transmembrane Protein 1 (LETM1) acts as a regulator of mitochondrial Ca2+ uptake distinct from the mitochondrial Ca2+ uniporter (MCU) pathway (Jiang et. al., 2009). However, a controversy exists regarding the function of LETM1 …
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Effect of oncomodulin on cytoskeletal actin dynamics.
… the actin-based cortical cytoskeleton. An EF-hand calcium buffering protein, oncomodulin (OCM) is preferentially expressed in OHCs and may aid in sculpting calcium signals important for actin remodeling. Targeted deletion of OCM leads to early progressive hearing loss in mice without severe …
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The characterisation of calpain-like proteins in Trypanosoma brucei
… lack the characteristic calcium-binding penta-EF-hand motif of typical vertebrate calpains and most of these novel calpain-like proteins are non-enzymatic homologues of typical calpains. The gene family is particularly expanded in ciliates and kinetoplastids, comprising 25 members in the …
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FKBP14 in Notch Signalling and Drosophila Development
… (Psn), with FKBP14 mutants exhibiting defects typical of loss of Notch signalling. FKBPs are highly conserved and involved in a wide array of biochemical processes including protein folding, assembly, and trafficking, yet they are non-essential in yeast and their role in development of …
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Molecular modelling of Androglobin
… not only be used to establish a common frame of reference between disorder prediction and fold recognition result, but it will also be integral to identifying the sites for potential MoRFs. Fold recognition techniques, when exposed to a query sequence, will produce tertiary structures homologous …
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
… and the small myristoylated, Ca2+ binding, EF hand protein, Frequenin (Frq1) facilitates its membrane localization (3). It has been suggested that Pik1 finds its substrate, phosphatidylinositol (PI), within phospholipid bilayers with assistance from the PI/PC transfer protein Sec14 (4). It …
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Metal sequestration of the host-defense protein human calprotectin
… to cause disease. As part of the first line of defense against pathogenic infection, the host innate immune response deploys a number of metal-chelating host-defense factors that restrict the access of nutrient metals from these microbes. One of these host-defense factors is the abundant …
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The Role of Munc18 Proteins in Physiologic and Pathologic Exocytoses in the Pancreatic Acinar Cell
… and Syn-3. In Chapter Five, I discovered a novel EF-hand Ca2+-binding protein called Cab45b, which binds Munc18b to regulate its membrane targeting and interactions with Syntaxins in the acinar cell in a manner that influenced Ca2+-induced amylase release. Taken together, these studies clarify our …
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Characterization of XVEF and XvCaM : two calcium-binding proteins isolated from the resurrection plant Xerophyta viscosa
… upon watering with minimal tissue damage. XVEF was isolated by differential screening of aX viscosa dehydration stress cDNA library. Reconstruction of the full length XVEF cDNA was conducted utilizing overlap extension PCR. XVEF codes for a putative calcium-binding protein and sequence …
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Die Bindung von Ca2+-Ionen an Zebrafisch-GCAP4
… dar. Diese wiederum gehören zur Superfamilie der EF-Hand-Calcium-Bindungsproteine. Von den vier EF-Händen ist bei NCS-Proteinen die erste nicht funktionell. Zudem sind die NCS-Proteine überwiegend N-Terminal myristoyliert. Beides gilt auch für GCAPs. Bisher wurden neun Mitglieder der GCAP-Familie …
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Biophysical Analysis of S100A12 and the Nucleosome Core Particle
… structural perturbations propagate allosteric effects across larger protein systems, using two distinct yet mechanistically analogous models: the calcium binding protein S100A12 and the nucleosome core particle. These studies are unified by a shared emphasis on how physical forces govern …
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Feedback Regulation of Wnt Signaling by Naked Cuticle (Nkd) During Drosophila Embryogenesis
… in mammals can cause diseases such as birth defects, cancer, osteoporosis, and diabetes. In Drosophila, the naked cuticle (nkd) gene antagonizes the Wnt/beta -catenin signaling in every segment of the embryo. Nkd is a modular, evolutionarily conserved protein that uses an EF-hand motif and …
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THE INTERPLAY BETWEEN HPCAL4 AND MECP2: IDENTIFICATION AND CHARACTERIZATION OF A NOVEL PUTATIVE TARGET FOR RETT SYNDROME THERAPY
… maturation and function. In fact, MeCP2 deficiency in neurons is responsible for impairment of fundamental aspects of neuronal development and function including defects in dendritic arborization and spine density. Besides Mecp2, several other genes have been associated with RTT or …
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Functional characterisation of the human mitochondrial ATP-Mg/Pi carriers and their disease-causing variants
… pool in the mitochondrial matrix, and therefore have a pivotal role in meeting the energetic demands of the cell. They consist of three domains; an EF hand-containing regulatory domain responsive to extra-mitochondrial calcium, an amphipathic helix, and a mitochondrial carrier domain, which …
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Molecular Determinant of Mitochondrial Shape Change
… evolutionarily conserved mitochondrial surface EF-hand domain containing Miro1. Glutamate and Ca2+ -stress driven cCa2+ mobilization cause MiST in neurons that is suppressed by expression of Miro1 EF1 mutants. Miro1-dependent MiST is essential for autophagosome formation that is attenuated in …
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