Global ETD Search
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Showing 1 to 14 of 14 for “"EF-Tu"”.
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EF-Tu Protein Domains:Functions and Thermostability
… genetiky a mikrobiologie Elongační faktor EF-Tu Funkce a termostabilita domén Disertační práce Hana Šanderová Specializace: Molekulární a buněčná biologie, genetika a virologie Školitel: Prof. MUDr. Jiří Jonák, DrSc. Oddělění genové exprese, Ústav molekulární genetiky AV ČR Praha 2008 …
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Untersuchungen zur stimulierten GTPase von EF-Tu aus Thermus thermophilus
… trägt, 780-fach schneller als GTP durch EF-Tu hydrolysiert. ii) Die geschwindigkeitsbestimmenden Schritte der DABP-GTPase und der GTPase unterscheiden sich. Bei der GTPase ist der nuklophile Angriff, bei der DABP-GTPase ein Protontransfer, vermutlich die Protonierung des gamma-Phosphates, …
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Evolution and dynamic behavior of transfer RNA in the first two steps of translation
… from the aaRSs and bind the elongation factor Tu (EF-Tu) for delivery to the ribosome. The recognition of specific tRNA species by the aaRSs, EF-Tu, and other enzymes along the translation pathway is based on sets of highly conserved nucleotides within different groups of tRNA species. Previous …
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Processes involved in protein translation
… change and the mechanism of elongation factor Tu (EF-Tu). The function and evolutionary history of both components are intertwined as the SSU is responsible for decoding information encoded in messenger RNA (mRNA) and EF-Tu is responsible for delivering amino acid building blocks to the …
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Structural And Functional Characterization Of Proteins Of Unknown Function (hp0495, Hp0100 And Hp1259) In Helicobacter Pylori
… of Gln and Asn. H. pylori elongation factor (EF-Tu) provides one such machinery, but it is not sufficient to maintain translational accuracy, suggesting the need for additional mechanisms. </p> <p>Hp0495 and Hp0100 were identified by yeast two-hybrid (Y2H) as potential new players in tRNA …
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The Development and Application of Methods to Study the Evolution of Specificity, Allostery, and RNA -Protein Interactions in Translation
… several archaea that eluded previous annotation studies. This enzyme was found to be involved in the indirect pathway of cysteine aminoacylation that is shown to exist in the last universal common ancestral state. We have developed a network analysis based on molecular dynamics simulations in …
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Analysis of protein-protein interactions linked to the formation of a bacterial cytoskeleton in Mycoplasma pneumoniae
… pneumoniae has a cytoskeleton-like structure. Based on genetic evidence, it was proposed that the 1818 amino acids long protein HMW2 plays a central role in both formation of the cytoskeleton-like structure and adherence to its host cell (cytadherence). As gene products of the hmw2 gene …
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Simulation and visualization of dynamics in RNA-protein complexes in translation
… tools were developed during the course of this study. We present the evolutionary analysis environment MultiSeq, dynamical network analysis and visualization, and a protocol for the preparation of RNA·protein MD simulations. For several Class I aminoacyl-tRNA synthetases (aaRSs), the rate …
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Untersuchungen zu Struktur-Funktionsbeziehungen in der tRNA-ähnlichen Struktur des Rüben-Gelbmosaik-Virus
… (nt) Länge umfaßt eine tRNA-ähnliche Struktur (TLS; 82nt) am 3´-Ende. Die vorliegende Arbeit beschäftigte sich mit dem Zusammenhang zwischen der Valylierung der tRNA-ähnlichen Struktur des Rüben-Gelbmosaik-Virus und der Replikation, bzw. Amplifikation des Virus. Es wurde der Frage …
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A novel function for the eukaryotic translation elongation factor 1A
The eukaryotic translation elongation factor 1A, eEF1A, a homolog of the bacterial EF1A (formerly known as EF-Tu), is a well-characterized ribosome associated factor, responsible for the delivery of aminoacyl-tRNAaa to the ribosomal A site. In contrast to this indirect interaction with the nascent …
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Der Translationsfaktor SelB
… SelB, dessen N-terminale Region Homologie zu EF-Tu zeigt und wie dieses Guanosin-Nukleotide und tRNA bindet. Der C-terminale Teil interagiert zusätzlich mit einer als SECIS-Element bezeichneten mRNA- Sekundärstruktur, die in Bakterien unmittelbar auf das für Selenocystein kodierende …
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Investigating the mechanisms of protein synthesis using multi-resolution structural data
… at ever increasing resolution. In order to study different aspects of ribosome function at the atomic level, we developed the molecular dynamics flexible fitting (MDFF) method that combines X-ray and cryo-EM data, furnishing atomic models of the ribosome corresponding to functional …