Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"ECM proteins"”.
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Cell engineering of human bone monolayers and the effect of growth factors and microcontact printed ECM proteins on wound healing. The role of ECM proteins, TGF¿-1, 2 and 3 and HCl/BSA in cellular adhesion, wound healing and imaging of the cell surface interface with the widefield surface plasmon microscope.
… and alignment to different micro-contact printed ECM protein patterns of different widths. The study also aimed at examining how TGF-¿1, 2 and 3 and their solvent and carrier (HCl and BSA, respectively) effected cell surface interactions, cell morphology, cell proliferation and integrin …
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Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins. Effect of transforming growth factor-beta (TGF-¿1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins.
Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. …
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Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins. Effect of transforming growth factor-beta (TGF-¿1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins.
Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. …
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Investigating the Molecular Mechanisms of SNED1 Fiber Assembly in the Extracellular Matrix
The extracellular matrix (ECM) is a complex scaffold of 150+ proteins that modulates all cellular functions and is critical for pathophysiological processes including cancer, and fibrosis. Our laboratory is interested in studying a novel ECM protein called SNED1 that Dr. Naba initially identified …
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Deformation mapping of fibronectin fibrils using digital image correlation
… produce large amounts of extracellular matrix (ECM) proteins that form a fibrillar scaffold on which bone mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of …
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Immune Responses Aginst Aldehyde Modified Extracellular Matrix in Atherosclerosis
… that modified surrounding extracellular matrix (ECM) proteins. These modifications may subsequently target immune responses against the plaque ECM and influence the atherosclerotic process. Our studies provide evidence for the presence of MDA-modifications on ECM proteins during atherosclerosis. …
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Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells
… responsible for degrading extra-cellular matrix (ECM) proteins in the local environment of a migrating cell. However, MT1-MMP has an intricate role in the regulation of the cell’s migration separate from its simple proteolytic functions. In addition, the increased expression of MT1-MMP has been …
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Enhanced Proteomic Methods to Decipher How the Novel Protein SNED1 Modulates the Extracellular Matrix
The extracellular matrix (ECM) is a complex meshwork of proteins that provides not only structural support but also biochemical cues to cells. The assembly of the ECM is a dynamic process requiring precise coordination: 1) ECM proteins are produced, post-translationally modified, and then secreted …
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Engineering Biosurfaces for Cell Studies
Biospecificity and the non-specific adsorption of proteins are two major issues for the use of biomaterials in implants and tissue engineering. This thesis describes efforts to reduce non-specific adsorption of proteins onto biomaterials and to enhance the biological activity of a biomaterial …
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The role of the tumour microenvironment components in cancer cell behaviour and drug response
… (CSCs) as well as the extracellular matrix (ECM), with the goal to develop an in vitro model that mimics solid tumours in terms of cellular characteristics and drug response. Mesenchymal stem cells were investigated as potential sources of cancer-associated fibroblasts (CAFs) in solid …
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VE-cadherin mechanotransduction signaling and its regulation by extracellular matrix
… Chapter 3 investigates how extracellular matrix (ECM) proteins and rigidity regulate the force-induced signaling pathway. In pathological situations like pulmonary fibrosis, the change of ECM can affect the endothelium permeability in response to mechanical perturbations. In this chapter, I showed …
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Analyzing Interactions Between Cells And Extracellular Matrix By Atomic Force Microscopy
… of cells with the extracellular matrix (ECM) have important roles in various physiological and pathological processes, including tissue morphogenesis during embryonic development, wound healing and tumor invasion. Although most of the proteins involved in cell-ECM interactions have been …
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Defining the role of heparan sulfate proteoglycans in APL-1 function in Caenorhabditis elegans
… the amyloid precursor protein (APP). APP family proteins are present in mammals and the family is considered necessary and essential for viability, although their exact function is still unclear. In <em>Caenorhabditis elegans</em> only one ortholog of <em>APP</em> is present, <em>apl-1</em>. …
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Engineering semiconductor quantum dots for quantitative imaging of cell motility and invasion
… binding to different extracellular matrix (ECM) proteins. QDs coated with poly-maleic anhydride (PMA), which had been modified to present alkane, alkene, alkyne, PEG and carboxylic acid, carboxylic acid, and solely PEG, were incubated on poly-l-lysine, collagen, fibronectin, and gelatin …
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Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues
… microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking protein scaffold with tunable biochemical, mechanical and topographical properties. This scaffold, formed by microfibres, displays …
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Fluid Flow Forces Regulate Fibrous Extracellular Matrix Protein Expression and Deposition Via A Rhoa/Rho Kinase Dependent Pathway During Atrioventricular Valve Development
… play a significant role in regulating fibrous proteins expression and deposition, which are critical components for embryonic cardiac valve morphogenesis. </p> <p>In chapter 2, our study demonstrated drastic increase of fibrous extracelluar matrix (ECM) proteins mRNA expression during cardiac …
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Laminar Flow-Based Microchemical Systems for Power Generation, Electrochemical Synthesis, and Biological Cell Studies
… surface gradients of extracellular matrix (ECM) proteins, laminin and collagen 1. These microfluidic platforms are capable of eliciting cell responses such as migration, proliferation, and differentiation, and offer the possibility of in vitro studies of a variety cell lines in response to …
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Proepicardial Cell Differentiation During Cardiac Development
… the deposition of fibrous extracellular matrix (ECM) molecules including periostin, tenascin, and collagen. The expression and localization of these ECM proteins is critical to the proper development and function of the heart. These phenomenons were inhibited by the addition of a TGFb family …
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Rational design to control multipotent stromal cell migration for applications in bone tissue engineering and injury repair
… the effects of tEGF and extracellular matrix (ECM) on MSC migration response and signaling. We take a systems level computational view to show a combined biomaterials and small molecule approach to control MSC migration. Cell migration is a delicately integrated biophysical process involving …
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The influence of ECM on mesenchymal stromal/stem cell activity and their interactions with monocytes/macrophages
… interactions and changing extracellular matrix (ECM) environment. This thesis aimed to extend the current understanding of the biology underpinning this process, focusing on the interaction between mesenchymal stromal/stem cells (MSCs) and monocytes/macrophages within dynamically changing ECM …
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