Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"ECIS"”.
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Extracellular Contractile Injection Systems (eCIS) - Characterization of Conserved Phage Tail-like Structures in Streptomyces
Extracellular contractile injection systems (eCIS) are bacteriophage tail-like structures produced by bacteria that have been implicated in diverse interactions with eukaryotic cells. Despite their distribution across many bacteria and archaea they have been relatively understudied and are often …
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How can elephant-caregiver interactions in zoos influence perceptions of elephant welfare states and emotions?
… explore how elephant-caregiver interactions (ECIs) that take place in zoos influence zoo visitors' perceptions of elephants' welfare states and emotions. This thesis employed purposive sampling to obtain participants and a pragmatic framework through which the research findings are engaged and …
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Investigations into endothelial cell permeability and adherens junction disruption induced by Junín virus infection.
… by electric cell-substrate impedance sensing (ECIS) and transwell assays. We also show that EC adherens junctions are disrupted during infection which may provide insight into the role of the endothelium in the pathogenesis of AHF and possibly other viral hemorrhagic fevers (VHFs).
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Nanoparticles for Simultaneous Assessment of Ros and Radiosensitization of Brain Cancer Cells for Improved Radiotherapy Outcomes
… available Electric Cell Impedance Sensor (ECIS) method. Irradiated T98G cells initially attached and migrated significantly (p<0.0001) more than non-irradiated cells in the first 20 hours post-irradiation and showed significant cell death thereafter. By calculating the fluorescence …
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The Cx43 Carboxyl-Terminal Mimetic Peptide αCT1 Protects Endothelial Barrier Function in a ZO1 Binding-Competent Manner
… Electric Cell-Substrate Impedance Sensing(ECIS), a TRITC-dextran transwell permeability assay, and a FITC-dextran cardiovascular leakage protocol involving Langendorff-perfused mouse hearts, αCT1 was found to protect the endothelium from thrombin-induced breakdown in cell-cell contacts. …
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Development of microelectrode arrays for real-time monitoring of alkaline phosphatase release from cells in clinical analysis
… an electric cell-substrate impedance sensing (ECIS) system. The integration of these techniques can enhance the detection of biological targets based on electrochemical changes in electrode interfaces. Our integrative approach took advantage of microelectrode arrays technology, facilitating the …
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Synergistically active curcumin resveratrol solid lipid nanoparticles for treatment of melanoma
… of curcumin, resveratrol and their combinations. ECIS measurements indicate that combination of curcumin and resveratrol has potential to stop cell migration of B16F10 melanoma cells. Both Cur-Res SLNs and solution of curcumin and resveratrol at the ratio of 3:1 demonstrated a strong synergism on …
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Bioimpedance spectroscopy of breast cancer cells: A microsystems approach
… on electric cell-substrate impedance sensing (ECIS) to monitor cell adhesion based events and the other was a microfluidic device with integrated microelectrodes to examine the biophysical properties of single cells. Second, we examined a panel of triple negative breast cancer (TNBC) cell lines …
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In vitro evaluation of Ultraviolet Radiation (UVR) exposure of the Retinal Pigmented Epithelium (RPE) and its relevance to Age-Related Macular Degeneration (AMD)
… upon specialist electrode culture plates (ECIS®, Applied Biophysics) which allow for electrostatic interrogation of key cell parameters, such as well coverage and tight-junction integrity, in a non-invasive fashion. Following from this, UVR exposures were repeated and a rapid cell viability …
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Repurposing the human immunodeficiency virus (HIV) integrase inhibitor raltegravir for the treatment of felid alphaherpesvirus 1 (FHV-1) ocular infection
… Then, electric cell-substrate impedance sensing (ECIS) was evaluated as a novel methodology to study the replication kinetics of herpesviruses. It was also used to characterize a fluorescently labeled FHV-1, created using CRISPR/Cas9 genome. Next, it was found that raltegravir inhibits both viral …
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Effects of Concurrent Radiosensitization and Chemoradiotherapy for Brain Tumors
… not show a significant increase in cell death, ECIS results show that there may be a decrease in migration and local invasion. This decrease in migration and local invasion may aid in reducing reoccurrence, a major contributor in death resulting from glioblastoma. The hypothesis is confirmed …
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Characterisation of a Novel GABA Signalling System in the Human Cerebral Vasculature
… calcium signalling by GABA receptor agonists. ECIS studies demonstrated that GABA receptor agonists do not play a role in modulation of pericyte contractility. Finally, early studies appear to indicate that ERK1/2 phosphorylation state in pericytes is not regulated by GABAB receptors, but this …
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Investigating viral subversion of intercellular communication
… cells were during adenoviral infection and by ECIS measurements of electrical resistance we demonstrate a transient increase in mechanical coupling during infection. Our future aims are to uncover changes in Coxsackievirus and adenovirus receptor (CAR) protein localization to determine if …
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La policía local en su intervención en materia de terrorismo yihadista.
… Queda constatada la importancia de los ECIs, como herramienta eficaz para la investigación a nivel internacional, pese a estar aún en una fase de iniciación, principalmente debido a la falta de experiencia en su utilización, por parte tanto de las fuerzas policiales como por la …
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Modeling and simulation of non-linear bioelectronic systems applied to cell culture assays
… In this thesis, we improve the accuracy of the ECIS (electrical cell-substrate impedance spectroscopy) technique of bioimpedance (BI) measurement of a monolayer cell-culture (CC), which measures the electrical response of a CC when an alternating current is applied to it at several frequencies. …
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Chemotaxis, shape and adhesion dynamics of amoeboid cells studied by impedance fluctuations in open and confined spaces
Die vorliegende Arbeit befasst sich mit elektrischen Impedanzmessungen von ameoboiden Zellen auf Mikroelektroden. Der Modellorganismus Dictyostelium discoideum zeigt unter der Bedingung des Nahrungsentzugs einen Übergang zum kollektiven Verhalten, bei dem sich chemotaktische Zellen zu einem …
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Where Electronics meets Biotechnology: Impedance-based devices for personalized medicine applications
L'abstract è presente nell'allegato / the abstract is in the attachment