Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"EBOV"”.
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Sphingomyelinases and Lysosomal Trafficking in Ebolavirus Entry
Ebolavirus (EBOV) is a negative sensed, single-strand filovirus which causes up to 90% mortality. Despite increasing outbreaks over the past decade, no approved vaccines or therapies exist. One drug treatment strategy is to target virus entry mechanisms. EBOV enters the cell using a …
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Bundibugyo Versus Zaire Ebolaviruses: Examination of the Polymerase Complex as a Therapeutic Target
… in geographic distribution: Zaire ebolavirus (EBOV), Sudan ebolavirus (SUDV), and Bundibugyo ebolavirus (BDBV). EBOV and BDBV are distinctly different in case-fatality rate, disease course, and clinical presentation in both humans and non-human primates, but the reasons for these differences …
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Description of interactions of Ebola virus with a putative reservoir species using next-generation sequencing
Ebola virus (EBOV) causes a severe, often fatal disease in humans and nonhuman primates. Recently, EBOV has caused two very large outbreaks, one of which is ongoing in the Democratic Republic of the Congo. Bats are the likely reservoir of EBOV, but little is known of their relationship with the …
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Antibody-based diagnostics and therapeutics for Zaire ebolavirus and Burkholderia pseudomallei
Zaire ebolavirus (EBOV) and Burkholderia pseudomallei are the causative agents of Ebola virus disease (EVD) and melioidosis, respectively. Early diagnosis is critical for halting the spread of the disease or providing treatment of the disease. Current diagnosis for EBOV includes polymerase chain …
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Development of Small Molecules as Broad-spectrum Filoviral Entry Inhibitors
Ebola (EBOV) and Marburg (MARV) filoviruses are priority infectious agents due to their high pathogenicity and lethality in infected patients. The current lack of pan-filoviral therapeutics exemplifies the need for effective treatments against diverse filoviruses. The filoviral glycoprotein (GP) …
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Metagenomic identification and characterisation of emerging and re-emerging viruses causing disease in febrile patients.
… to EID. Plasmodium, Leptospira and Ebola virus (EBOV) infections were detected in Sierra Leonean patient samples using PCR-based assays. Human immunodeficiency virus, GB virus C (human pegivirus) and hepatitis B virus were identified in 36 PCR-negative Sierra Leonean patient samples using …
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Aptamers that bind filovirus GP : a new frontier for detection and neutralization of Ebola and Marburg virus
… that differentially recognize GPs from MARV and EBOV. This work represents the first step in the development of aptamer based-low-cost point of care devices for filovirus disease diagnostics. Finally, this work describes the use of a hybrid selection approach that combines two different selection …
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Activation of the TLR4 Signalling Complex by the Ebola Virus Glycoprotein
… the first expression of native and stable Zaire EBOV GP in HEK293 under BSL2 conditions with native folding, glycosylation, and full activity. Additionally, to allow the exploration of ideal expression conditions for native GP, multi-step protein purification strategies were developed to ensure …
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Characterization of Ebola virus VP35-dsRNA binding for drug development against Ebola virus disease.
The Ebola virus (EBOV) VP35 protein plays an important role in the inhibition of the initial innate immune responses to EBOV infection leading to Ebola virus disease development. In fact, VP35 interaction with the RIG-I like receptors (RLR) cascade components inhibits the interferon (IFN) …
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Mapping the Major Epitope(s) of the Glycoprotein of Ebolavirus with QB Phage Display System
… phage virions containing various segments of the EBOV glycoprotein fused to its A1 the readthrough minor coat protein. The recombinant phages would then be utilized to analyze their antigenicity and to map the major epitope(s) of the glycoprotein determinants. This research study represents a …
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Proviral Roles of Ebola virus VP35 Ubiquitination
… regulated. We generated recombinant EBOVs encoding glycine (G) or arginine (R) mutations at VP35/K309 (rEBOV-VP35/K309G/-R) and show that both mutations prohibit VP35/K309 ubiquitination. The rEBOV-VP35/K309G mutant loses the ability to efficiently antagonize the IFN-I response, while …
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Elucidating the Immunogenicity and Protective Immune Response of a Trivalent Vaccine for Sudan Ebola, Marburg, and Lassa Fever Viruses
Sudan Ebola (SUDV), Marburg (MARV), and Lassa fever (LASV) viruses cause acute, severe haemorrhagic fevers resulting in high morbidity and mortality in overlapping regions of Africa. Despite millions of individuals in Africa being at risk of contracting an infection from one of these viruses, …