Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"E2 conjugating enzyme"”.
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Targeted Degradation of Intracellular Proteins using Ubiquitin Variant Induced Proximity
… as inhibitors and activators of Ub related enzymes, targeting protein-protein interaction surfaces that are often elusive to chemical inhibitors. Building upon this foundation, we hypothesized that UbVs could serve as fusion protein components to facilitate targeted protein degradation—a …
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Design and synthesis of molecular tools for the ubiquitin proteasome system
… a mutant form of ubiquitin, Ubb+1, and the E2-conjugating enzyme E2-25K, has been identified as playing a possible pathophysiological role in the early and late stage progression of AD. The E2-25K/Ubb+1 protein-protein interaction (PPI) is regulated by the α-helix 9 of E2-25K. Creating a …
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The Role Of Ubc9 In Drug Resistance And Its Expression Regulation In Cancer Cells
… as well as cancer development. As an essential E2 conjugating enzyme for sumoylation, Ubc9 plays a central role in sumoylation-mediated cellular pathways. In this study, we investigate the role of Ubc9 in drug resistance as well as its regulation in cancer cells. An early gene product, Gam1, …
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Effects of altered expression of the sumo conjugating enzyme, UBC9 on mitosis, meiosis and conjugation in Tetrahymena thermophila
… modification in eukaryotic species. Ubc9p is the E2-conjugating enzyme for SUMOylation and consequently it influences multiple cellular pathways. Nuclear proteins are common targets of SUMOylation and regulate nuclear events such as transcription, DNA repair and mitosis. The segregation of the …
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Discovery and Effects of Pharmacological Inhibition of The E3 Ligase Skp2 By Small Molecule Protein-Protein Interaction Disruptors
… associates with Cullin1 and Rbx1 to bridge the E2 conjugating enzyme with its protein substrate to execute its E3 ligase activity. Skp2 is an Fbox protein (due to it containing an Fbox domain) and it is the rate-limiting component of the SCF complex. Skp2 targets several cell-cycle regulatory …
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Understanding T cells in type 1 diabetes: a role for c-Maf and characterization of intracellular signaling following engagement of transgenic Ly49A.
… is facilitated by direct interaction with the E2 conjugating enzyme Ubc9 and increases following T cell stimulation. In addition, c-Maf physically interacts with p65/RelA. This interaction is dependent on the DNA binding domain of c-Maf and phosphorylation of p65 at serine 536. In transfected …
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Functional Interactions of E2~Ubiquitin Conjugates in and outside the Ubiquitination Pathway
… control, and DNA damage repair. Although the enzymes within the Ub transfer pathway are well established (E1, E2, E3), the molecular details required for protein ubiquitination are largely unknown. To address questions regarding the final stages of Ub transfer, we began with structural …
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Structural Insights into TRIM21 Ubiquitination Mechanisms
… During ubiquitination, RING E3 ligases direct E2 Ub-conjugating enzymes to substrates to catalyze their ubiquitination. The cytosolic antibody receptor TRIM21 possesses unique ubiquitination activity that drives broad-spectrum anti-pathogen targeting and underpins the protein depletion …