Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"E. coli DNA"”.
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Synthesis and Biological Evaluation of 2'-Deoxyribonucleotide Analogs
… for 2'-deoxyadenosine-5'-triphosphate in DNA nick translation by E. coli DNA polymerase I.
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Structural Analysis of Dna Complementary to Bovine Parathyroid Hormone Messenger-Rna and Restriction Endonuclease Mapping of the Parathyroid Hormone Gene
… (PTH), full length single and double stranded DNA complementary (cDNA) to bovine PTH mRNA was synthesized by reverse transcriptase and E. coli DNA polymerase I. Single stranded cDNA migrated as a single discrete band of 750 nucleotides by denaturing gel electrophoresis. Hybridization of the …
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Einfluß der Chromatinumgebung auf die Genregulation durch den Transkriptionsfaktor Sin4 aus der Hefe Saccharomyces cerevisiae
… ein zentrales Thema der Molekularbiologie. Die DNA eukaryonter Zellen liegt durch Bindungen an Histon-Proteine im Zellkern als Chromatin vor. Die Chromatinstruktur dient nicht nur der Komprimierung der DNA auf engstem Raume, sondern hat auch starke Auswirkungen auf die Funktion der DNA. So …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
<p>DNA polymerases maintain the genome integrity from one generation to the next by faithfully synthesizing new DNA and by participating in DNA repair processes. The Klenow fragment of E. coli DNA polymerase I has served as a model polymerase for decades because it is straightforward to purify, …
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An investigation of the development and evaluation of online approaches for improved kinaesthetic learning in science
… the Lac Operon gene regulation system in E. coli, DNA transcription and translation, and Salmonella virulence factors. A mixed-methods study was conducted including three pilot studies, three randomised control trials and two sub-studies. Study participants included over 100 students from a …
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Stochastic modeling of inducible logical gates in Escherichia coli
… and/or lac operator sites by a non-binding E. coli DNA sequence. The individual components of the promoter were then characterized by studying the output GFP signal when varying inducer concentrations were added to the system. In addition, we have characterized systems with mutant tet operator …
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Effects of Green Tea Extract (GTE) for Normal and Cancer Cell Death by Radiation
… 항산화 및 항암 효과는 여러 논문에서 보고되고 있으며 또한 감마선에 의해 유도된 E-Coli DNA 절단에 대한 보호효과가 있음이 보고되었다. 일본에서 보고된 암 예방 및 암 재발 감소효과와 앞서 실행된 상당수의 실험연구를 근거로 녹차추출물의 암 예방 물질로서의 가능성을 알아보기 위한 임상연구가 진행중이다. 본 연구에서는 방사선을 조사한 정상세포와 종양세포에서 녹차추출물의 효과를 알아보고자 하였다. 본 실험에서는 마우스의 정상 소장 상피세포주인 IEC-6과 인간 대장암세포주인 HCT-116을 사용하였다. 방사선조사는 6MV …
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Fingerprinting and characterisation of Escherichia coli isolates using DNA arrays
Two commercially available DNA whole genome Escherichia coli K12 arrays were compared to identify a subset of markers for typing. The arrays were identical in probe composition but different in substrate (membrane and glass slide arrays) and probe preparation (radio- and fluorescent-labelled). …
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Attenuation of Corynebacterium pseudotuberculosis by targeted mutagenesis of the phospholipase D gene.
… all three PLD-associated activities on E. coli. DNA sequence analysis revealed that both inserts encode a 307 amino acid protein which, in secreted form, has a predicted a molecular weight of 31.1 kDa and a pI of 8.84. The primary structure of the corynebacterial PLDs were compared to those …
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Synthetic approaches to the understanding of DNA nucleobase methylation
DNA methylation is a major source of genetic variation and cancer. Methylation occurs when nucleophilic DNA bases react with methylating agent methyl methanesulfonate (MMS), dimethyl sulfate (DMS), N-methyl-N-nitrosourea (MNU), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), etc. …
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Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
… platform with distinct advantages to both cDNA arrays and commercial arrays produced by in situ synthesis of multiple short oligonucleotides per gene. However, gene expression analysis using microarrays of long oligonucleotides is limited in that it requires substantial amounts of RNA. The …
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Study of mechanism of genetic death in Escherichia coli in response to A) dTTP starvation and B) combination of temperature sensitive defects in replication initiation and double strand break repair
… death of the cell, or by blocking the process of DNA replication and segregation (without affecting protein/RNA/lipid metabolism) – which we classify as genetic death of the cell. Irreparable DNA lesions, therefore, inflict genetic death on the cells, and we are interested in identifying such DNA …