Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"Dynamin"”.
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Yeast Dynamin Functions with Clathrin at the Golgi
… protein and a yeast ortholog to the mammalian dynamin-like protein (Dlp1). Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. Classical dynamin family proteins work in concert with clathrin to facilitate …
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A Role for Dynamin-Dependent Endocytosis During Drosophila Gastrulation
… present work that demonstrates a requirement for Dynamin-dependent endocytosis during these contraction events. Using quantitative analysis, I have identified varied cell behaviors during experiments which knockdown the function of <em>dynamin</em>. In addition, I demonstrate the existence of an …
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Functional Connection Between Yeast Dynamin and Retromer at the Endosome
… and Alzheimer's disease. Vps1, a yeast dynamin-like protein, plays a role in intracellular trafficking. Vps1 has been shown to localize at the endocytic sites to promote pinching off of endocytic vesicles. The goal of this study was to further investigate the relationship between the …
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Identifizierung und funktionelle Charakterisierung von Dynamin B aus Dictyostelium discoideum
Dynamine sind große GTPasen, die in der Zelle an wichtigen Prozessen wie der Endocytose, dem Vesikeltransport, der Morphologieerhaltung und Verteilung von Mitochondrien u.v.m. beteiligt sind. Welche Rolle die Dynamine hierbei im Detail spielen, ist jedoch noch ungeklärt. Im Rahmen dieser Arbeit …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
… derived vesicles with the late Golgi body. The dynamin-like GTPase Vps1p is required for vacuolar sorting and the late Golgi-retention of some proteins. It is known that the endocytic trafficking defect caused by the loss of VPS1 is essentially identical to cells that had lost RCY1. I …
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Die GTPase Dynamin A : strukturelle und biochemische Hinweise auf einen molekularen Motor
Die GTPasen der Dynamin-Familie besitzen eine einheitliche Domänenstruktur, erfüllen aber sehr unterschiedliche biologische Funktionen, wie die Vesikelgenerierung in der Endocytose, den Erhalt der Mitochondrienmorphologie und die Abwehr von viralen Pathogenen. Während die biologische Funktion …
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Röntgenstrukturanalyse der GTPase-Domäne von Dynamin 1 und der Motordomäne von Myosin II
… konnten die Strukturen der GTPase-Domäne von Dynamin 1 aus Rattus norvegicus sowie der Motordomäne von Myosin II aus Dictyostelium discoideum in atomarem Detail aufgeklärt werden. Die vorgelegte Kristallstruktur der nukleotidfreien GTPase-Domäne von Dynamin 1 zeigt, daß die GTPase-Domäne in …
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Analysis of the Interaction and Functional Determinants of Arc and Its Regulation of Dynamin
… may be mediated by its reported interaction with dynamin 2, a large (~100 kDa) GTPase that participates in receptor-mediated endocytosis, actin polymerization, and microtubule stabilization. The effects of Arc on dynamin activities have not been characterized. Therefore, I have purified …
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Genetic Dissection of Synaptic Vesicle Endocytosis
… A key protein in this recycling process is dynamin. Dynamin is a GTPase that catalyzes the scission of a budding endosome off its parent membrane. The mammalian brain expresses three isoforms of dynamin. Using genetically modified mouse hippocampal neurons, I analyzed the functional …
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Dissecting Synaptic Vesicle Endocytosis
… We examined the role of the large GTPases dynamin 1 and dynamin 3 in synaptic vesicle endocytosis; which have been thought to be essential for the fission of budding vesicles from the plasma membrane. Dynamin 1, 3 and 1/3 double knockouts indicated that there is redundant presynaptic …
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Characterization of the entry mechanisms utilized by the alphavirus venezuelan equine encephalitis virus to infect mosquito cells
… known as Rab proteins and the large GTPase dynamin. These proteins have been shown to play a role in the entry of several viruses and are thought to be involved in alphavirus entry in mammalian cells. Here mosquito homologs of these proteins are identified, isolated and characterized in the …
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Dynamins and myosin-II regulate the distinct modes of synaptic vesicle exocytosis in mature cerebrocortical nerve terminals and this involves calcium dependent phosphorylations.
… The results show that the inhibition of dynamins can increase the FM2-10 dye release during certain stimulation conditions (4AP5C and ION5C; where 5C represents 5 mM [Ca2+]e) without changing the Glu released indicating that dynamin(s) are required for the closure of the fusion pore (and …
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Pharmacological insights into C-‐C motif chemokine receptor 5 mediated chemotaxis
… was involved in chemotaxis. Inhibition of dynamin, Grb2 and non-‐receptor tyrosine kinases were equally effective at blocking migration in THP-‐1 cells but less effective at blocking CXCL11 stimulated migration in activated PBLs. Conclusions: This study has shown that CCL3 stimulated …
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Phosphorylation sites on specific neuronal proteins can control the mode of synaptic vesicle exocytosis and thereby regulate synaptic transmission
… 5 mM Ca2+. In this model employed, myosin-II and dynamins can regulate the closure of the fusion pore of the readily releasable pool (RRP) of SVs during KR but are independent of each other’s actions. In biochemical assays, synaptosomes were maximally labelled with FM2-10 dye such that both the …
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Cellular determinants of reovirus entry and infection
… caveolin-1 revealed that ISVPs use dynamin-dependent caveolar endocytosis to enter host cells. These studies also showed that reovirus virions were able to take advantage of caveolar endocytosis to infect cells. Because many viruses have recently been found to enter cells through …
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Molecular basis of plant cell death suppression by the phytophthora infestans effector AVR3a
… and mass spectrometry analysis. A host GTPase (dynamin) protein involved in endocytosis was found. Dynamin was shown to be required for ICD suppression activity by AVR3a. Notably, dynamin accumulates around P. infestans (Pi) haustoria possibly pointing to its role in the plant-Pi interaction.
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Hexagonal packing of Drosophila wing epithelial cells by the Planar Cell Polarity pathway
… a process that depends on both the large GTPase Dynamin and the function of Rab11 recycling endosomes. We suggest that E-cadherin is actively internalized and recycled as wing epithelial cells pack into a regular hexagonal array. Hexagonal packing furthermore depends on the activity of the Planar …
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Regulation of Synaptic Vesicle Trafficking at Central Synapses
… impact of one of main players in endocytosis, dynamin in different forms of release. Acute inhibition of dynamin in central synapses impairs activity-dependent synaptic vesicle recycling while leaves spontaneous recycling intact, suggesting the operation of two parallel recycling pathways in …
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VPS1 on Dynamics of Endocytic Components and Endocytic Scission
The yeast dynamin-related protein Vps1 shares high homology with dynamin in its N-terminal GTPase and C-terminal GED domains. To test the significance of two conserved domains in early endocytosis, an endocytic maker, Abp1, was fused with GFP in three GTPase mutants (vps1K42E, vps1S43N, and …
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