Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"Drug selection"”.
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Molecular control of embryonic stem cell identity
… in producing pluripotent cells.In these studies, selection for the activation of the markers Oct4 or Nanog led to completely reprogrammed cells, but selection for fbx15, a downstream target of Oct4, resulted in partially reprogrammed intermediates. An unresolved issue in the field was whether …
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
… cell to differentiate into melanocyte and confer drug resistance and green fluorescence as well. Highly enriched differentiating melanocytes were obtained using this system by drug selection.
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Genotypes of SNPs of Key Genes Regulate Susceptibility and Drug Sensitivity to Neovascular AMD
… though their efficacy varies across different drugs and individuals, highlighting the importance of precise drug selection in clinical outcomes. Research suggests that factors such as baseline vision, age, disease duration, lesion size, central retina thickness, neovascularization type, and …
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Using Capillary Electrophoresis for High-Throughput Molecular Screening
… chemical libraries have necessitated rapid selection strategies to aid in molecular discovery. Encoded chemical libraries are composed of thousands of unique (potential) drug molecules, each encoded with a corresponding unique DNA sequence, allowing for the simultaneous screening of ligands …
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An Expandable Stage-Based Machine Learning and Statistical Pipeline for Detecting Adverse Drug Reaction Risks on Complex Multidimensional Datasets
Adverse Drug Reactions (ADRs) are a persistent challenge to patient safety, often escaping detection in aggregate analyses. This research introduces expandable, modular pipeline that identifies statistically significant drug–ADR associations through a multivariable, propensity-adjusted framework …
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Making augmented human intelligence in medicine practical: A case study of treating major depressive disorder
… In this dissertation, we assert that treatment selection and management can be individualized when clinicians assessment of disease symptoms are augmented with a few analytically identified patient-specific measures (e.g., genomics, metabolomics) that are prognostic or predictive of treatment …
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Expression Analysis and Stem Cell Engineering
… ES cells were genetically engineered to permit drug selection of subset of ES derived neural cells. We hypothesized that engineering ES cells with puromycin acetyltransferase gene: PAC) under the control of the Olig2 promoter would allow for selection of Olig2 expressing neural cells with …
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Rational drug combinations design against intratumoral heterogeneity and clonal evolution
… select pre-existing resistant subclones toward drug resistance. Mathematical/computational modeling from population genetics, evolutionary dynamics, and engineering are being utilized to a greater extent in recent times to study tumor progression, intratumoral heterogeneity, drug resistance, and …
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Chemotherapy-Eluting Intraperitoneal Implants for Advanced Stage Ovarian Cancer Treatment
… microparticulate cisplatin, a widely used drug in OC treatment. The material was designed to match mechanical properties of human abdominal organs for safe long-term IP placement. Sheets fabricated from the composite material were evaluated in vitro for sustained cisplatin release and for …
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The potential of antimicrobial resistance diagnostics to inform prudent antimicrobial use in feedlot cattle: dynamic models as tools for optimizing interventions in bovine respiratory disease
… animals. The misuse and overuse of antimicrobial drugs in agricultural settings as elsewhere accelerates the selection of resistant pathogens; emerging antimicrobial resistance (AMR) threatens the therapeutic efficacy of available antimicrobials, leading to treatment failures, production losses …
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Topical pharmacokinetics for a rational and effective topical drug development process
Topical drugs are not developed by the same process as oral drugs. The process is more uncertain and contains gaps. This leads to a poor discharge of risks before going to the clinical phases. The topical drug development process is reviewed in the introduction of the thesis. In particular, past …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… and <italic>ADE1</italic> and a standardized selection of at least 16 unique restriction sites in their polylinkers. Additionally, I have expanded the range of drug selection options that can be used for PCR-mediated homologous replacement using pRS plasmid templates by replacing the …
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Engineering mammalian cell line to improve sialylation
… integrated into CHO cells using a plasmid with a drug selection marker to produce stable cell lines. It is found that the Pol III promoter is not strong enough to generate sialidase siRNAs. The modified CMV promoter is more appropriate for knocking down sialidase activity as clones with over 50% …
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Crystallographic, Molecular Dynamics, And Enzymatic Studies Of Multi-Drug Resistant Hiv-1 Protease And Implications For Structure Based Drug Design (project 1); Crystallographic Studies Of Human Myelin Protein Zero (project 2)
<p>Under drug selection pressure, emerging mutations render HIV-1 protease drug resistance, leading to the therapy failure in anti-HIV treatment.Tthe multidrug-resistant 769 (MDR) HIV-1 protease (resistant mutations at residues 10, 36, 46, 54, 62, 63, 71, 82, 84, 90) is selected for the present …
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New approaches to antimalarial target deconvolution and measuring fitness effects of Plasmodium falciparum mutations implicated in drug resistance.
… control efforts face the persistent challenge of drug resistance and, to date, the malaria parasite has been able to develop resistance to almost every drug with which it has been challenged. One strategy to tackle the problem of drug resistance is to systematically identify new antimalarials to …