Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Drosophila Larvae"”.
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High-Throughput Operant Conditioning in Drosophila Larvae
… mechanisms are still not fully understood. The Drosophila larva is an excellent model system to study neural circuits, since it is genetically tractable, with a variety of tools available. Although it is quite small, it is capable of a diverse range of behaviours and can achieve complex learning …
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Fatty Acid Auxotrophy in Drosophila Larvae Lacking SREBP
… a major characteristic of larval development in Drosophila melanogaster. Such growth presumably requires the concomitant production of membrane lipids and is also accompanied by a significant accumulation of neutral lipid stores. Growing larvae must accumulate fatty acids to permit the synthesis …
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Damage-Induced Inflammation and Nociceptive Hypersensitivity In Drosophila Larvae
… inflammation and pain hypersensitivity using Drosophila larvae. To study wound-induced inflammation, we generated transgenic larvae with fluorescent epidermal cells and blood cells (hemocytes). Using live imaging, we monitored the circulatory dynamics of hemocytes and the methods by which they …
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An investigation of nutrition-dependent mRNA translation in Drosophila larvae
… used polysome gradient analysis in developing Drosophila larvae to show that starvation led to a rapid decrease in translation, seen as early as 30 minutes after removal of larvae from food. A maximal decrease in translation was observed after 6-18 hours of starvation. Using qRT-PCR I have …
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Cellular and Genetic Bases of Cold Nociception and Nociceptive Sensitization In Drosophila Larvae
… using the genetically tractable <em>Drosophila</em> model. Using a novel "cold probe" tool and assay we found larvae produce a mutually exclusive set of reactive behaviors to a defined noxious cold stimulus (3-12 ºC), including a full-body contraction and the bending of anterior and …
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Cracking the Odor Code: Molecular and Cellular Deconstruction of the Olfactory Circuit of Drosophila Larvae
<p>The Drosophila larva offers a powerful model system to investigate the general principles by which the olfactory system processes behaviorally relevant sensory stimuli. The numerically reduced larval olfactory system relieves the formidable molecular and cellular complexity found in other …
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Microfuidic Devices and Open Access Tool for Localized Microinjection and Heart Monitoring of Drosophila Melanogaster
… current research gaps associated with the use of Drosophila larvae as an in-vivo model for cardiac toxicity and cardiac gene screening. In objective 1, we have developed a hybrid multi-tasking microfluidic platform that enables desired orientation, reversible immobilization, and localized …
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Sterol requirements in Drosophila melanogaster
… fluidity and microdomain formation. Drosophila is an excellent model system in which to study functional requirements for membrane sterol because, although it does not synthesize sterol, it nevertheless requires sterols to complete development. Moreover, Drosophila normally …
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Visualizing Roles of Spastic Paraplegia Proteins in Organizing Axonal ER in Live Drosophila
… I have therefore aimed to develop these tools in Drosophila larvae and adults, and use them to visualize mutant phenotypes. Firstly, I developed a system to visualize fluorescently marked ER in individual axons in adult fly legs, and tested how this can be used to investigate the effects of loss …
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Investigating the Role of Insulin-Like Signaling in Paclitaxel-Induced Nociceptive Hypersensitivity using Drosophila melanogaster
… sensory neuron damage in human patients and in Drosophila larvae. We used a modified feeding protocol to establish a genetically tractable Drosophila model of PTX-induced hypersensitivity. Larvae fed PTX exhibited hypersensitivity to thermal and mechanical stimuli. Thermal hypersensitivity was …
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How Neuronal Stem Cells Acquire and Maintain their Identity
… in mammals is still very fragmented. Within Drosophila, four different types of progenitors with partially identified regulator gene networks generate the brain. Of interest are Type II NSCs, whose progeny build the central complex, the functional analogue of the vertebrate hippocampus. A …
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Transcriptional Regulation of Profilin Is Required For Drosophila Larval Wound Closure
… change their behavior to migrate across it. In Drosophila larvae, wound closure requires two signaling pathways (the Jun N-terminal kinase (JNK) pathway and the Pvr receptor tyrosine kinase signaling pathway) and regulation of the actin cytoskeleton. In this and other systems, it remains unclear …
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Genetics and Evolution of Drosophila Humoral Immunity against Parasitoid Infections
… boulardi is a major natural parasite of Drosophila melanogaster. However, the regulation of Drosophila humoral immunity against parasitoids is not clearly illustrated. In this thesis, I investigated the humoral immune mechanisms underlying Drosophila resistance to parasitoid infections, …
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Starved State Dependent Modulation of Olfactory Receptor Neuron Function in Drosophila melanogaster larvae
… we exposed starved or non-starved third instar Drosophila larvae to specific odorants to analyze the effect of individual ORN activity on chemotaxis. We used two different behavioral paradigms to analyze the chemotaxis response of larvae to odorants. When tested with odorants that elicit strong …
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Development of leg motor neurons in drosophila melanogaster
Drosophila larval and adult stage forms are very different. Drosophila larvae move using undulatory body muscle contractions, while adult flies walk and fly using legs and wings. Leg motor neurons control multi-jointed leg movement by coordinately regulating leg muscle contractions. With the aim of …
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Behavioural principles underlying navigational decision-making in Drosophila melanogaster larvae
… level and across entire brain circuits. The Drosophila melanogaster larva is an apt model system for probing the mechanisms of decision-making given its rich behavioural repertoire, small nervous system, genetic tractability, and available neuronal wiring diagrams. Taking inspiration from the …
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Octopaminergic Regulation of Olfactory Discrimination in the Drosophila Larval Mushroom Body Calyx
… sVUM1 neuron 1st-order upstream neurons in the Drosophila first-instar (L1) connectome, I found three classes of neuron synapsed onto the sVUM1 neurons in their dendritic region within the SEZ: one class of neurons originated in the protocerebrum; a second class were local SEZ interneurons with …
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The Octopaminergic Modulatory Circuitry of the Drosophila Larval Mushroom Body Calyx
… aimed to use the simple and genetically amenable Drosophila larval Mushroom Body (MB) calyx, a higher order processing area involved in learned odour discrimination, as a model to map octopamine (OA) neuromodulatory circuitry. I first identified olfactory projection neurons (PNs), a GABAergic …