Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Drosha"”.
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Characterization of Inducible SMC-specific Drosha Knockout Mice
… of this study was to investigate the function of Drosha, another of the two RNase III proteins in miRNA biogenesis, in intestinal SMCs using a transgenic animal model. We generated SMCspecific inducible Drosha null animals (smMHCCre-ERT2/+;Droshalox/lox) by cross-breeding a smMHC/Cre/eGFP …
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Understanding Recognition of Primary MicroRNAs by Human Drosha/DGCR8
… by suppressing messenger RNAs. The RNase Drosha, as part of the Microprocessor complex, performs the initial processing step in miRNA biogenesis. Drosha, in complex with its partner protein DGCR8, must distinguish its RNA substrates (called primary microRNAs) and identify the proper …
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Investigating MDM2 Regulation of Endothelial Cell Angiomir Biosynthesis in Response to High Glucose Treatment
… to high glucose concentrations enhances MDM2:DROSHA binding and represses DROSHA expression both in vitro and in diabetic mice (db/db) muscles. Treatment with MDM2 inhibitors repealed glucose-induced DROSHA downregulation. This suggests that high glucose concentrations downregulates DROSHA …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… primary (pri-)miRNA transcripts are processed by Drosha complex into precursor (pre-)miRNAs, and then by cytoplasmic Dicer complex into mature miRNAs. The processing of pri-miRNAs is the most highly regulated step in the miRNA biogenesis pathway. Therefore, understanding the molecular mechanisms …
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Hypoxia Mediated Downregulation of Mirna Biogenesis Leads to Increased Tumor Progression
… biogenesis by downregulation of two key enzymes, Drosha and Dicer in cancer cells. Previous data from our laboratory had shown the clinical relevance of downregulated Dicer and Drosha in ovarian and other cancer types, but a clear mechanistic understanding is needed for future clinical …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… short hairpin structures by the cleavages of DROSHA, a RNase III enzyme, in the nucleus and DICER, another RNase III enzyme, in the cytoplasm. endo-siRNAs are distinguished from miRNAs in that endo-siRNAs are processed from naturally occurring long dsRNAs and the biogenesis of endo-siRNAs is …
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Peripubertal Binge Ethanol, Age and Sex Modulate Microrna Expression in the Ventral and Dorsal Hippocampus of the Adolescent Rat
… (nt), single-stranded microRNAs involves Drosha and Dicer enzymatic processing of microRNA precursors. A mature microRNA imperfectly base pairs with messenger RNA (mRNA) target genes,which leads to mRNA cleavage or translational repression. Our lab has found that repeated binge EtOH …
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Expression analysis of Drosophila melanogaster microRNAs
… Biogenese zu untersuchen. dmDGCR8 (Pasha), ein Drosha- interagierendes Protein, konnte so als neuer Faktor identifiziert werden, der an der nukleären Prozessierung von pri-miRNAs beteiligt ist. Es konnte gezeigt werden, daß dmDGCR8 und Drosha Depletierung zur pri-miRNA Akkumulation in der Zelle …
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A survey of fly and nematode small RNAs by deep sequencing
… two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a pre-miRNA hairpin, then Dicer cleaves near the loop to generate a miRNA:miRNA* duplex. Large-scale sequencing of cDNAs derived from endogenously expressed small RNAs is used here to examine the …
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The Epstein-Barr Virus-Encoded BHRF1 miRNAs Regulate Viral Gene Expression in Cis
… expression. Processing, by the RNAse III protein Drosha, of the BHRF1 miRNAs out of EBNA-LP transcripts, as part of the miRNA biogenesis pathway, allows the BHRF1 miRNAs to regulate EBNA-LP expression in cis. We used a meta-analysis of RNA-Seq data and performed PacBio sequencing to show that …
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Sequence determinants of pri-miRNA processing
… by the Microprocessor, a complex containing Drosha, an RNase III enzyme, and an RNA-binding partner DGCR8. However, it is unclear how the Microprocessor distinguishes between these hairpins and authentic pri-miRNAs. In fact, C. elegans pri-miRNAs are not processed in human cells, illustrating …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
… effects of depleting microRNA biogenesis enzyme, DROSHA, and microRNA effector proteins, AGO1-4 and TNRC6A-C, on microRNA expression. Our findings help to define the boundaries of RNAi in a widely used cell line, HCT116, by refining a subset of abundant microRNAs that are DROSHA-dependent, …
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The Regulation of Microrna Biogenesis By Ribosome-Interacting Proteins
… (referred to as pri-miRNA) which are cleaved by Drosha and DGCR8 to generate 60-90 nucleotide precursor miRNA. The precursors are cleaved again by Dicer and loaded into the RNA-induced silencing complex (RISC) of which Argonaute 2 is the functional component. Many of the proteins involved in …
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Enhancement of Dicer as a personalized anticancer strategy
… like Dicer, TAR RNA-binding protein (TRBP) and Drosha. Herein, the enhancement of miRNA processing as a therapeutic strategy is explored. Developing small molecule or peptide based therapeutics that mimic TRBP, an endogenous activator of Dicer, to enhance miRNA processing in cancers with reduced …
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Mutational Landscape of Noncoding Ultraconserved Elements in Human Cancers: Functional Relevance and Clinical Utility
… including MIR142, whose mutations affect the Drosha-mediated processing of the pre-miR-142 by preventing SRSF3 binding to the CNNC motif in the 3p flanking sequence, resulting in a downregulation of the mature sequences of miR-142. To explore potential clinical utility, we develop a targeted …
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SR proteins in microRNA/mRNA biogenesis
… assay, I showed that SF2/ASF can promote the Drosha cleavage step of pri-miR-7 through directly association with the predicted binding site. So far, this is the first SR protein discovered, which is directly involved in miRNA biogenesis. Moreover, our preliminary data also suggested that …
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Elucidation of the Role of miR-184 in the Development and Maintenance of the Drosophila Melanogaster Nervous System
… pri-miRNAs are processed by the endonuclease Drosha into a hairpin secondary structure (pre-miRNA), exported from the nucleus and cleaved by the enzyme Dicer to form a duplex RNA molecule. This miRNA:miRNA* duplex is subsequently further processed to form a single-stranded, mature miRNA. …
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Diverse RNA processing pathways important for post-transcriptional gene regulation
… characteristic stem-loop precursors. Cleavage by Drosha defines the premiRNA hairpin, which is then cleaved by Dicer to generate a mature miRNA. This biogenesis pathway ensures high fidelity definition of miRNA 5' ends, which determine target specificity. Small RNAs from Caenorhabditis elegans and …
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
… nuclear endoribonuclease and homolog of human Drosha with well-characterized roles in the processing of ncRNAs. The enzyme recognizes and cleaves double-stranded RNA stems containing a terminal tetraloop with an AGNN consensus sequence. However, the scope and consequence of its function in mRNA …
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The Roles of miR-155 and miR-15/16 in Natural Killer Cell Function and Maturation
… that are subsequently 'cropped' by the Drosha/Dgcr8 complex into precursor miRNAs (pre-miRNA) that have a characteristic stem-loop structure. The pre-miRNA is exported to the cytoplasm where it is further processed by Dicer1, yielding a mature 18-24 nucleotide miRNA. The mature miRNA is …
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