Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Droplet digital PCR"”.
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The Effects of Known PCR Inhibitors on Droplet Digital PCR Performance
… that can inhibit the polymerase chain reaction (PCR). Inhibitors associated with forensic samples include substances that are endogenous with the sample (calcium, melanin), absorbed from the environment (humic acid, indigo dye)1, or introduced during laboratory processing (phenol1 , glove …
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Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment
… of HIV-1 DNA quantitation in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative breastmilk samples were spiked with 8E5 …
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Bartonella in Small Mammals from Montana and Idaho
… overall prevalence of approximately 37% based on droplet digital PCR (ddPCR) screening. Following ddPCR screening, individuals that yielded positive results were selected for further analysis including genetic sequencing of the RNA polymerase b-subunit (<em>rpoB</em>) gene. While 116 individuals …
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Antibiotic Resistance Genes in Marquette County Michigan's Wastewater
… resistance profiles. A Qiagen Microbial DNA qPCR plate screened for 87 different ARGs and detected 41, most encoding beta-lactam resistance. Ten ARGs were quantified using droplet digital PCR: bla<sub>CTX-M</sub>, bla<sub>TEM</sub>, bla<sub>CMY-2</sub>, bla<sub>SHV</sub>, KPC, NDM, OXA-48, …
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Evaluating Determinants of Mitochondrial Mutagenesis and their Intersections with Cell Fate
… effects on mtDNA frequency with newly developed droplet digital PCR assays. Here I found that these classic nuclear mutagens damage mtDNA, but are not capable of significant mtDNA mutation induction. Next, I evaluated the cellular effects resulting from expression of a mitochondrial-targeted form …
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Identificazione e Validazione di Hotspot di Metilazione del DNA come Biomarcatori per il Melanoma Cutaneo
… of both RARB and ISL1 were evaluated by using droplet digital PCR (ddPCR) while the methylation levels of the DNA methylation hotspots computationally identified were assessed using a custom protocol developed by the Laboratory of Experimental Oncology of the University of Catania defined …
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Advancing detection, genomics, and secretome analysis of oomycete pathogens Phytophthora capsici and Phytopythium vexans to explore mechanisms of pathogenesis and biocontrol solutions
… the development of an amplitude-based multiplex droplet digital PCR (ddPCR) assay. Designed for simultaneous quantification of P. capsici, Trichoderma asperellum, and Trichoderma gamsii in soil, this assay was validated in field trials and offers high sensitivity and specificity. The assay …
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Investigating the heritability of resistance fueling copy number variation in glyphosate resistant Amaranthus palmeri
… gene CN estimates were obtained by probe-based droplet digital PCR and the copy number estimates were used to perform controlled crosses to create the F1 generation. The results illustrate an overall increase in mean copy number in response to increasing mean EPSPS copies in the parents. I …
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Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques
… temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against …
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Biomarcatori circolanti per ictus in pazienti con cancro: studio pilota
… e amplificazione basato su un sistema droplet digital PCR (ddPCR) ad alta sensibilità. I dati prodotti sono stati analizzati considerando le caratteristiche cliniche e patologiche dei pazienti per identificare nuovi efficaci biomarcatori epigenetici associati a differenti quadri …
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microRNAs in Uveal Melanoma: in vivo findings and computational analysis
… levels of the three miRNAs under investigation. Droplet digital PCR (ddPCR) was used for both liquid biopsy and tissue samples. A novel computational analysis for the three selected miRNAs was performed in order to corroborate the experimental results. Results: Significantly higher expression …
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The microbiology of chronic suppurative otitis media
… abundance of clinically relevant bacteria. Droplet digital PCR (ddPCR), amplicon sequencing and multiplex PCR were utilised in this study. The distribution of the bacteria within the tissue was examined using histology and Gram staining. Genera Staphylococcus and Pseudomonas, the main …
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Molecular Analysis of Breast Cancer Transcriptomes, Genomes, and Circulating Tumor DNA
… rearrangements that were confirmed by PCR. Circulating tumor DNA levels across multiple plasma samples during the clinical course were analyzed by quantitative droplet digital PCR. Accurate post-surgical discrimination of EM patients (93%) from DM (100%) was achieved by ctDNA …
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Analyzing Extracellular Vesicles as Potential Biomarkers of Stroke Using Polymer Microfluidic Devices
… min). Gene expression analysis performed via droplet digital PCR revealed that for AIS, the genes we selected (PLBD1, MMP9, VCAN, FOS, CA4) pro-duce similar expression between the CD8+ T cells and EVs originating from these cells. The analysis of clinical samples, which used a 7-bed …
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KINETICS AND PROTEOLYTIC SIGNATURES OF HISTATIN 5 DEGRADATION IN SALIVA AND GINGIVAL CREVICULAR FLUID IN PERIODONTAL HEALTH AND GINGIVITIS
… degrading histatin 5 in clinical samples using droplet digital PCR (ddPCR); and (4) comparing proteomic profiles of histatin 5 degradation between clinical and in vitro settings. In vitro, only P. gingivalis degraded histatin 5, producing 40 distinct fragments within 6 hours. Clinically, …
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A Forward Genetic Screen Revealed the Role of POLDIP2 in Ensuring Maternal Inheritance of Mitochondrial DNA in Drosophila melanogaster
… Moreover, I performed genetic crosses and droplet digital PCR and indicated that the retained paternal mtDNA could be transmitted to F1 progeny. Biochemical assays showed that POLDIP2 localises to the mitochondrial matrix and interacts with ClpX, a chaperone-like subunit of the major …
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Molecular Pathology: Potential Biomarkers For The Detection Of Down Syndrome Pregnancies
… biomarkers for DS. We will also use multiplex droplet digital PCR (ddPCR) using cell-free fetal DNA (cffDNA) to target different genes on chromosomes 21, 18, and 13 to screen for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13), respectively. PRSS56 and SPINK7 proteins are secreted …
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Identificazione di nuovi biomarcatori epigenetici per la diagnosi precoce del carcinoma colorettale
… di amplificazione ad alta sensibilità della droplet digital PCR (ddPCR). Dopo le analisi ddPCR, il potenziale diagnostico di quattro miRNA selezionati, hsa-miR-21-5p, hsa-miR-497-5p, hsa-miR-503-5p e hsa-miR-375 è stato valutato attraverso analisi statistiche e ulteriori valutazioni …
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Assessment of the clinical validity of ctDNA Analysis for melanoma management
… plasma ctDNA extraction and quantification using droplet digital PCR (Chapter 2). Using this methodology, we evaluated the ctDNA detection rate in untreated BRAF mutant melanoma patients, as a potential alternative to tumour genotyping (Chapter 3), where the potential economic benefit of …
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Wastewater-based Surveillance for Hepatitis A and C in an Inflow- and Infiltration-Impacted Rural System
… measured within 24 hours of sample collection. Droplet digital PCR (ddPCR) was performed in triplicates to quantify viral targets HAV, HCV, SARS-CoV-2, and the human fecal indicator crAssphage. The likelihood of missed detection at the WWTP influent for HAV and HCV compared to the sub-sewershed …
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