Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Droplet Microfluidics"”.
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Dissecting Pluripotency and Mammalian Embryonic Development via Droplet Microfluidics
Pluripotency, the ability of a cell to differentiate towards any type of somatic cell is a transient feature of the developing embryo. In vitro, pluripotency can be captured in the form of embryonic stem cells (ESCs). In this study, I developed a microfluidic-based system to encapsulate ESCs into …
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Silicon-based microfabricated droplet-microfluidics electrospray emitter for neurochemical sensing
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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Double emulsion droplet microfluidics and high-throughput measurements of single viruses
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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BACTERIA ANALYSIS BY USING A SUPERVISED MACHINE LEARNING ALGORITHM BASED ON DROPLET MICROFLUIDICS
… cell detection and quantification. By using droplet microfluidics and a machine learning algorithm, the objective of this study was to propose a technology that analyzes images of bacterial cells by image processing and Support Vector Machines algorithm to classify droplets containing the …
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Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
… rare (1 in 105 on average). Ultrahigh-throughput droplet microfluidics has emerged as an effective technology to overcome this limitation, with the ability to screen 107 reactions per day. However, fewer than ten enzyme assays have been implemented in droplets to date and only once has droplet …
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Experimental studies of liquid marbles and superhydrophobic surfaces
The interaction of water droplets with hydrophobic or rough, superhydrophobic solid surfaces has been studied. Such surfaces may be found in the natural world and their potential applications range from waterproof and self-cleaning surfaces to droplet microfluidics. A measure of hydrophobicity is …
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Microvortices In Droplets: Generation & Applications
<p>The emerging field of droplet microfluidics deals with the manipulation of nL-fL droplets encapsulated within an immiscible carrier phase. The droplets are used as reaction containers for biochemical assays, enabling drastic reduction in assay volumes needed for modern life sciences research. To …
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Profiling, prototyping, and perturbing human immune responses
… perturbation of human immune cells using droplet microfluidics. In addition, we apply this framework to identify immune phenotypes associated with sepsis and severe COVID-19, and develop an experimental system that models sepsis-induced emergency myelopoiesis using human cells.
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Exploring the emergence of pesticide degradation in the α/β hydrolase superfamily
… otherwise inaccessible sequence space, I use droplet microfluidics to screen large libraries of enzyme variants in high throughput. Within only two rounds of evolution, P91’s phosphotriesterase activity could be increased by 400-fold. The improved enzyme displays a catalytic efficiency close …
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3D Cell Culture in Microgels: A Novel Screening Platform for Disease Modelling and Drug Testing
… to encapsulate CRC organoids in microgels via droplet microfluidics to enable new assays applicable for drug screening. To make the organoid-microgel system applicable to combinatorial drug screening, I developed a barcoding method to couple treatment information to the microgels. By using …
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Application of Argon Plasma Technology to Hydrophobic and Hydrophilic Microdroplet Generation in PDMS Microfluidic Devices
… Technology to Hydrophobic and Hydrophilic Microdroplet Generation in PDMS Microfluidic Devices Brennan Graham Microfluidics has gained popularity over the last decade due to the ability to replace many large, expensive laboratory processes with small handheld chips with a higher throughput due …
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Quantitative analysis of protein liquid-liquid phase separation
… was implemented by combining the technology of droplet microfluidics, fluorescent imaging, and quantitative computational analysis. This new approach, replacing manual step-wise experiments, enables to generate phase diagrams with minimal sample and time consumption as well as high resolution. …
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Experimental and numerical study of temperature-actuated droplets within microfluidics
Droplet microfluidics (DM), which involves the production of nano-/micro- droplets and particles using immiscible phases, reveals an impressive evolutionary trend that have been widely used to establish highly sensitive, robust, and flexible multitasking microsystems. Droplets generated by DM …
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Probing biomolecular reactions and self-assembly in microfluidic systems for quantitative measurements and therapeutic applications
Microfluidics involves the confinement of fluid to small length scales, which makes it possible to conduct experiments which have no corollary in the bulk regime. In this thesis, microfluidic technology is used to enable quantitative measurements of proteins and other biomolecules. In addition, …
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Electric field based fabrication methods for multi-scale structured surfaces
… critical to understand how moving liquid water droplets will behave when they encounter these modified surfaces. We investigate drop impingement on porous thin films produced by BDA and EPD in order to understand the effects of surface structure and chemical properties on droplet dynamics. Using …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
… three-dimensional agarose microgels generated by droplet microfluidics. The three-dimensional matrix of the microgels supported the naïve pluripotency network and upregulated Plakoglobin. A cell-adhesion protein located in both adherens junctions and desmosomes. It is important for tight cell-cell …
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Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
… of small-volume compartmentalisation afforded by droplet microfluidics are combined with DNA circuitry, to achieve isothermal and enzyme-free single-molecule DNA-sensing in a digital droplet assay. This approach is applied to the field of molecular computing, where the assay functions as a highly …
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Generating artificial metalloenzymes containing new-to-nature organometallic cofactors via controlled ligand exchange and microfluidic screening technologies
… from the aqueous phase when used in water-in-oil droplet microfluidics. The fourth chapter focuses on the formation of ArMs from organometallic complexes. A systematic study is presented, describing how the steric and electronic properties of a ruthenium piano-stool complex, carrying a key …
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Microfluidic-generated Double Emulsions for Cell Study, Drug Delivery and Particle Therapeutics Fabrication
<p>Droplet microfluidics is a powerful platform for both fundamental and applied biomedical research. The droplets are small in size with a diameter of 1-300 um. Thus, they could function as a miniaturized environment for quantitative and qualitative analysis. Each droplet composes of water …
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