Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Droplet Digital PCR (ddPCR)"”.
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Bartonella in Small Mammals from Montana and Idaho
… overall prevalence of approximately 37% based on droplet digital PCR (ddPCR) screening. Following ddPCR screening, individuals that yielded positive results were selected for further analysis including genetic sequencing of the RNA polymerase b-subunit (<em>rpoB</em>) gene. While 116 individuals …
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The Effects of Known PCR Inhibitors on Droplet Digital PCR Performance
… that can inhibit the polymerase chain reaction (PCR). Inhibitors associated with forensic samples include substances that are endogenous with the sample (calcium, melanin), absorbed from the environment (humic acid, indigo dye)1, or introduced during laboratory processing (phenol1 , glove …
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Advancing detection, genomics, and secretome analysis of oomycete pathogens Phytophthora capsici and Phytopythium vexans to explore mechanisms of pathogenesis and biocontrol solutions
… the development of an amplitude-based multiplex droplet digital PCR (ddPCR) assay. Designed for simultaneous quantification of P. capsici, Trichoderma asperellum, and Trichoderma gamsii in soil, this assay was validated in field trials and offers high sensitivity and specificity. The assay …
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Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques
… temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against …
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Biomarcatori circolanti per ictus in pazienti con cancro: studio pilota
… e amplificazione basato su un sistema droplet digital PCR (ddPCR) ad alta sensibilità. I dati prodotti sono stati analizzati considerando le caratteristiche cliniche e patologiche dei pazienti per identificare nuovi efficaci biomarcatori epigenetici associati a differenti quadri …
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microRNAs in Uveal Melanoma: in vivo findings and computational analysis
… levels of the three miRNAs under investigation. Droplet digital PCR (ddPCR) was used for both liquid biopsy and tissue samples. A novel computational analysis for the three selected miRNAs was performed in order to corroborate the experimental results. Results: Significantly higher expression …
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The microbiology of chronic suppurative otitis media
… abundance of clinically relevant bacteria. Droplet digital PCR (ddPCR), amplicon sequencing and multiplex PCR were utilised in this study. The distribution of the bacteria within the tissue was examined using histology and Gram staining. Genera Staphylococcus and Pseudomonas, the main …
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KINETICS AND PROTEOLYTIC SIGNATURES OF HISTATIN 5 DEGRADATION IN SALIVA AND GINGIVAL CREVICULAR FLUID IN PERIODONTAL HEALTH AND GINGIVITIS
… degrading histatin 5 in clinical samples using droplet digital PCR (ddPCR); and (4) comparing proteomic profiles of histatin 5 degradation between clinical and in vitro settings. In vitro, only P. gingivalis degraded histatin 5, producing 40 distinct fragments within 6 hours. Clinically, …
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Molecular Pathology: Potential Biomarkers For The Detection Of Down Syndrome Pregnancies
… biomarkers for DS. We will also use multiplex droplet digital PCR (ddPCR) using cell-free fetal DNA (cffDNA) to target different genes on chromosomes 21, 18, and 13 to screen for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13), respectively. PRSS56 and SPINK7 proteins are secreted …
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Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment
… of HIV-1 DNA quantitation in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative breastmilk samples were spiked with 8E5 …
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Identificazione di nuovi biomarcatori epigenetici per la diagnosi precoce del carcinoma colorettale
… di amplificazione ad alta sensibilità della droplet digital PCR (ddPCR). Dopo le analisi ddPCR, il potenziale diagnostico di quattro miRNA selezionati, hsa-miR-21-5p, hsa-miR-497-5p, hsa-miR-503-5p e hsa-miR-375 è stato valutato attraverso analisi statistiche e ulteriori valutazioni …
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Identificazione e Validazione di Hotspot di Metilazione del DNA come Biomarcatori per il Melanoma Cutaneo
… of both RARB and ISL1 were evaluated by using droplet digital PCR (ddPCR) while the methylation levels of the DNA methylation hotspots computationally identified were assessed using a custom protocol developed by the Laboratory of Experimental Oncology of the University of Catania defined …
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Wastewater-based Surveillance for Hepatitis A and C in an Inflow- and Infiltration-Impacted Rural System
… measured within 24 hours of sample collection. Droplet digital PCR (ddPCR) was performed in triplicates to quantify viral targets HAV, HCV, SARS-CoV-2, and the human fecal indicator crAssphage. The likelihood of missed detection at the WWTP influent for HAV and HCV compared to the sub-sewershed …
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Investigating drivers of cyanobacterial blooms in Aotearoa – New Zealand lakes using sedimentary ancient DNA
… ancient DNA (sedaDNA) using metabarcoding and droplet digital PCR (ddPCR) in Chapter 2. Bloom-forming species were present in all lakes prior to human arrival; however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement …
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Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma
… V2, and selected mutations were validated using droplet digital PCR (ddPCR). For ctDNA, the frequencies of genes affected by non-synonymous somatic mutations included TP53 (67%), KRAS (11%), PIK3CA (2%) and SF3B1 (2%). A total of 41 gene variants were identified in either pre- or post-NACT …
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MONITORING OF LEISHMANIA TARENTOLAE AND LEISHMANIA INFANTUM IN SAND FLIES AND SYNANTHROPIC REPTILES OF NORTHERN ITALY: EMERGING EPIDEMIOLOGICAL SCENARIOS?
… DNA. To address these challenges, we used droplet digital PCR (ddPCR) to develop the assay. The method was validated using dog blood spiked with DNA from various isolates of both Leishmania species, as well as DNA extracted from wild-caught sand flies previously tested for Leishmania …
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Identificazione del pattern di espressione dei microRNA nei pazienti con cancro del colon-retto e sua modulazione favorevole dopo l'arricchimento del microbiota intestinale con i probiotici
… metodica di amplificazione ad alta sensibilità Droplet Digital PCR (ddPCR) è stato possibile valutare la disregolazione dei livelli di espressione dei quattro miRNA selezionati sia in campioni di biopsia liquida che in campioni di tessuto paraffinato (FFPE) di tumore e mucosa normale adiacente …
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Circulating tumour DNA: A non-invasive biomarker for melanoma
… To do this we developed a new and innovative ddPCR TERT mutation assay and investigated its sensitivity alongside current assays in detecting mutations in melanoma tissue containing a small fraction of tumour cells. The significance of ctDNA for patient monitoring relative to current methods …