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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Dragline"”.
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Molecular dynamics analysis of supercontraction in spider dragline silk
Spider dragline silk is a material that has evolved over millions of years to develop finely tuned mechanical properties. It is a protein-based fiber, used as the main structural component in spider webs and as a lifeline for the spider, and it combines strength and extensibility to give it …
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
… mechanical and biological properties. Dragline silk has been the most prominent focus of research because of its exceptional high tensile strength and extensibility. In our research, we have focused on the characterization of small molecular weight proteins found within dragline and …
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Sequence-structure correlations in the MaSp1 protein of N. clavipes dragline silk
… MaSp1 protein from the N. clavipes spider dragline silk sequences are reported, obtained using an in silico approach based on replica exchange molecular dynamics (REMD) and explicit water molecular dynamics. In particular, the atomistic simulations discussed in this parametric study explore …
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Characterization of a family of cysteine rich proteins and development of a MaSp1 derived miniature fibroin
… it one of the toughest materials on the planet. Dragline silk, also known as the lifeline of the spider, represents one of the best studied fiber types and many labs are attempting to produce synthetic dragline silk fibers for commercial applications. In these studies, we develop a minifibroin …
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Western North Dakota lignite strip mining processes and resulting subsurface characteristics
… a waste bank were observed to depend upon the dragline cutting pattern, distance between dragline positions, the previous mining activity adjacent the dragline position, mass wasting, overburden stratigraphic sequence, breaking characteristics of the overburden sediments, dragline bucket motion …
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Structural heterogeneity in silk fibers and its effects on failure mechanics and supercontraction
Spider dragline silk is a protein material that has evolved over millions of years to become one of the strongest and toughest natural fibers known. Silk features a heterogeneous structure that comprises [beta]-sheet crystals embedded in an amorphous matrix. However, it is not fully understood how …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… the world. Of the seven different spider silks, dragline silk, the lifeline silk of the spider, represents one of the most renowned fiber types that has extraordinary properties. As a result, many labs across the globe are racing to manufacture synthetic dragline silk fibers. With the production …
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The conserved C-terminal domain of spider tubuliform spidroin 1 contributes to extensibility in synthetic fibers
… of studies have focused on the production of dragline silks from synthetic spider silk gene products. Here we report the first mechanical analysis of synthetic egg case silk fibers spun from the Latrodectus hesperus tubuliform silk proteins, TuSp1 and ECP-2. We provide evidence that …
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Preferential nanoreinforcement of thermoplastic polyurethane elastomers with dispersed nano-clay
… that are both strong and tough like spider dragline silk. Inspired by the morphology of spider dragline silk and motivated to develop strong, tough, elastomeric polyurethanes to be used in soldier applications I have prepared polyurethane/clay nanocomposites. Polymer/clay nanocomposites have …
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Correlations between physical and structural properties of spider silk - Correlaties tussen fysische en structurele eigenschappen van spinnenzijde
… voor twee types spinnenzijdes (eizak- en “dragline” spinnenzijde) in vergelijking met zijdes afkomstig van twee rupsen Bombyx mori (of gewone zijde) en Antheraea pernyi (Tussah of wilde zijde). Tijdens dit onderzoek werd vastgesteld dat eizakspinnenzijde een totaal ander mechanisch gedrag …
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Evidence that aciniform silk and minor ampullate silk are major constituents of wrapping silk from the black widow
… have been performed on the following silks: dragline, capture spiral, and eggcase silk. The primary goal of this study was to characterize a lesser known silk type, called wrapping (swathing) silk, from <em>Latrodectus hesperus</em>. My research focused on elucidating the fibroins that make …
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The Synthetic spider silk fibers spun from Pyriform Spidroin 2, a glue silk protein discovered in orb-weaving spider attachment discs
… a viscous liquid that rapidly solidifies, gluing dragline silk fibers to substrates for locomotion or web construction. Here we report the identification and artificial spinning of a novel attachment disc glue silk fibroin, Pyriform Spidroin 2 (PySp2), from the golden orb weaver Nephila c/avipes. …
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Characterication of aggregate gland silk factor 1
… and toughness values that are below natural dragline silk fibers. Secondary structural analyses of the AgSF1 recombinant proteins in solution using circular dichoism reveal the N-terminal region of AgSF1 is alpha helical in nature. Collectively, these studies advance our understanding of silk …
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Herstellung und Charakterisierung von Fasern aus rekombinanten Spinnenseidenproteinen und deren potentielle Applikationen
… Luftfeuchtigkeit, wobei zum Vergleich natürliche Dragline und nassgesponnene Fäden aus eADF3(AQ12), eADF3(AQ12NR3) und eADF3(AQ24) einbezogen wurden. Ferner wurden, um den Einfluss der Garnmorphologie auf die hygroskopischen Eigenschaften evaluieren zu können, die Polymere Polycaprolacton und …
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Funktion und Einfluss der nicht-repetitiven, terminalen Domänen auf Speicherung und Assemblierung von Spinnenseidenproteinen
… als Abseilfaden verwendet wird. Sie wird als dragline-Seide bezeichnet und ist hauptsächlich aus zwei Proteinklassen aufgebaut, die als MaSp1 und MaSp2(Major Ampullate Spidroin) bezeichnet werden. Die mehrere hundert Kilodalton großen Spidroine bestehen im Wesentlichen aus einer repetitiven, …
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Expression, purification and characterization of the structural properties of recombinant Pysp1 and Pysp2 spidroins
… produces gluelike threads functioning to cement dragline silk to substrates. We focused on the molecular properties of PySp 1, the major component of pyrifonn silk from Latrodectus hersperus, and its putative Oiiholog, PySp2, from Nephi/a clavipes. To date, there are no reports describing the …
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Expression, purification, and characterization of a novel cysteine-rich silk protein expressed in the tubuliform and aggregate glands of the black widow spider : a thesis
… tryptic digestion of black widow spider dragline silk, the most studied type of silk, identified a novel cysteine-rich nonfibroin- like peptide that we named cysteine-rich component or CRC- 1. Further analysis of a large pool of nucleic acid sequences deposited in our custom eDNA database …
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Generation of cDNA chips from the black widow spider, latrodectus hesperus, for gene discovery and expression profiling using microarray technology, and molecular characterization of a novel silk glue protein
… or indirectly involved in the manufacture of dragline silk. Microarray analyses also enable the discovery of several other interesting molecules, two of which could be accessory proteins involved in silk formation. Furthermore, in a separate study we also characterized a novel silk glue …
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Imaging, Mechanics, Construction, and Sonification of Three-Dimensional Spider Webs
… of the interplay between nonlinear behavior of dragline silk and the complex and redundant structure of the web in the mechanical and functional performance of the web. We investigate and quantify the structure and mechanics of a 3D spider web at varying stages of construction. This is …
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