Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Dppa2"”.
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Xenopus DPPA2 is a Direct Inhibitor of Microtubule Polymerization Required for Nuclear Assembly
… the contribution of the DNA-binding protein Dppa2 to nuclear assembly. In Dppa2-depleted extracts, nuclei are small and deformed, assemble incomplete nuclear envelopes and fail DNA replication. We show that Dppa2 directly depolymerizes microtubules, and must be localized on DNA to tune local …
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The roles of DPPA2, DPPA4 and SMARCA5 in mouse zygotic genome activation, epigenetic reprogramming and development
… (mESC) identified the small DNA binding proteins DPPA2, DPPA4 and the chromatin remodeler SMARCA5 as potential inducers of major ZGA. Here I focus on validating these three factors and I assess their roles in vivo, using conditional knock-out mouse models and a targeted protein depletion system, …
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Investigating the role of DPPA2 and DPPA4 in the Epigenetic Control of Lineage Programs in Human Embryonic Stem Cells
… I discover a role for the transcription factors DPPA2 and DPPA4 in maintaining poised chromatin in human pluripotent stem cells (hPSCs). I found that DPPA2/4 bind to the majority of CpG islands, poised promoters and a large subset of poised enhancers. CRISPR-Cas9 mediated knockout of DPPA2/4 in …
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CRISPR activation screening at single-cell transcriptomic resolution: discovering new regulators of zygotic genome activation
… the top hits, I identify the DNA binding protein Dppa2, the chromatin remodeller Smarca5 and the transcription factor Patz1. I subsequently validate these top hits using both CRISPRa and cDNA overexpression in mESCs. By doing this, I uncover new factors that promote a ZGA-like response in mESCs …
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Studies on approaches for sequencing cytosine modifications and the identification of their protein readers
… screen identified the epigenetic priming factors Dppa2 and Dppa4 as combination-specific interactors of 5-hydroxymethylcytosine, though the mechanism of these proteins’ interaction with 5-hydroxymethylcytosine requires further work to be conclusively determined.
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Single-cell analyses of cellular reprogramming and embryonic stem cells
… analysis, we found Esrrb, Utf1, Lin28 and Dppa2 to be predictive markers of reprogramming. We found that single cells exhibit high variation in gene expression early in reprogramming and this heterogeneity decreases are the cell reaches pluripotency. Our results show that a stochastic phase …
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An immunoproteomic approach to identifying cancer-associated autoantibody biomarkers
… candidates for melanoma included CEACAM 1, DPPA2, FGFR2, ITGB1, MAGEA10, NANOG, PIM1, SPANXB1, THEG and XAGE1B. Using these biomarker candidates, distinct clusters of HCs and melanoma patients were identified in both unsupervised hierarchical clustering and PCA analysis. Combinatorial ROC …