Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Downstream Purification"”.
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Optimization of Downstream Purification of Lucentis
A base case for the production and purification of the fragmented antibody, Lucentis, was explored and compared to several alternative cases that were hypothesized to optimize the purification process. The base case consisted of using a series of chromatography columns including anion exchange, …
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Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco
… protein yield- and activity, and impact downstream purification. In this study we aimed to compare expression levels using both Zea mays (maize) and Nicotiana benthamiana (tobacco) with relevant vector technologies. We expressed GRFT in maize using an endosperm specific maize expression …
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Development of a continuous-flow synthesis of neostigmine methylsulfate and studies toward a continuous-flow synthesis of lisinopril
… Yuqing Cui and Dr. Naomi Briggs developed the downstream purification sequence. Dr. Nopphon Weeranoppanant developed the in-line evaporator and, along with Dr. Dale Thomas, assisted with performing the synthesis within their developed frame. Liam P. Kelly developed the continuous synthesis of …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… process, including upstream cell culture, downstream purification and analytical characterisation. A CHO-DP12 cell line engineered for anti-IL-8 mAb expression and a cNIST mAb-producing CHO cell line were employed to investigate key cell culture process parameters. Upstream studies examined …
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The development of novel excipients for the stabilization of proteins against aggregation
… will likely be useful during production and purification for improving yield and lowering downstream purification costs.
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Evaluation of the financial impact of continuous chromatography in the production of biologics
… financial impact of changing one or more of the downstream chromatography steps involved in the purification of a complex biologic molecule from a batch process to a continuous one. This is particularly relevant, as biologics represent the fastest growing segment of the human therapeutics market, …
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The functionalisation and application of microporous micro-capillary films for the chromatographic purification of biomolecules
… be a solution to the growing bottleneck in the downstream purification of biopharmaceuticals. However, MMCFs have been mainly tested for binary separations of model proteins and broader capabilities of this technology remain largely unknown. The experimental work presented in this thesis focused …
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Performance and Fouling during Bioreactor Harvesting
… proteins are the most challenging impurities for downstream purification processes. In order to investigate the fouling during cell clarification, HCPs in the bioreactor, harvest, and backwash are identified and quantified using different methods. A dataset is established using the identified HCPs …
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Quality by design for the downstream processing of biopharmaceuticals
… an optimal or preferred Protein A resin for the purification of a monoclonal antibody at industrial scale. Resins identified for closer scrutiny are used to purify a biopharmaceutical monoclonal antibody and the product of the resins is compared to identify a Protein A resin to act as the basis …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… the gene sequence for a his6x tag to assist with downstream purification as well as a horse radish peroxidase (HRP) gene. Cloning scFvs into this vector allows their fusion to HRP, resulting in the production of potential reagents for use as secondary antibodies in western blots and ELISAs. The …
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
… coli, driven by upstream cultivation scales and downstream purification complexities. Annual OPEX varies from USD 0.36 million to USD 2.34 million, yielding unit manufacturing costs (COGM) of USD 102–1 182 per mg when normalized to output. Low-titre enzymes like DNase I (5 mg/L) and VCE (10 mg/L) …