Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"Downstream Processing"”.
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Quality by design for the downstream processing of biopharmaceuticals
… is the most commonly used technology for downstream purification of biopharmaceuticals, tangential flow filtration (TFF) is probably the second most common unit operation in the manufacture of these products. To determine how QbD can be applied to TFF a case study is developed in applying …
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Addressing the Downstream Processing Challenges Within Manufacturing of Oncolytic Rhabdoviruses
… way to full regulatory approval. At present, the downstream processing of OVs relies on a combination of chromatography and membrane-based processes to remove process-related (e.g. host-cell proteins and nucleic acids) and product-related impurities (e.g. aggregated virus particles). This thesis …
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Downstream processing of recombinant and endogenous proteins from livestock milk
… milk with high resolution and high yield while processing about 10-20 grams in a single operation. The second process(RCFE) was able to isolate the active sub-populations of rhPC from major milk contaminants( - and -pig casein) as wells as from the inactive sub-populations of rhPC. RCFE was able …
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Downstream processing of Black soldier fly larvae protein for human consumption
… aesthetic appeal and marketability. Developing a downstream process to partially remove undesirable colour and odours without compromising nutrition is essential to enhance consumer acceptance of BSFL protein products, especially in Western markets projected to represent 14% of global demand by …
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Direct exposure of linguistic principles in model architectures for downstream processing
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
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Downstream Processing with Affinity Chromatography. A Study of a Continuous Process for Biospecific Adsorption
A polymer matrix of hydroxy ethyl methacrylate - ethylene glycol dimethacrylate was synthesised and coupled with the ligand p-aminobenzamidine. Although the adsorbent displayed sufficient affinity towards trypsin, the overall adsorption performance was not satisfactory. Subsequent studies were …
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Characterization and application of vortex flow adsorption for simplification of biochemical product downstream processing
… a biochemical product is simplification of downstream processing. Biochemical product recovery often starts from fermentation broth or cell culture. In conventional downstream processing, the initial steps are clarification, concentration, and purification. Simplification of downstream …
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Yeast and plant cells as biofactories for recombinant proteins : evaluation of novel expression and downstream processing strategies
… this thesis were achieved by the expression and downstream processing of recombinant therapeutic proteins from P.pastoris fermentation broth and N.tabacum BY-2 suspension cells. These results demonstrate the use of yeast and plant cells as biofactories for the production of therapeutic and …
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Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
Recombinant human P450s are important in preclinical studies to discover possible interactions between drugs when taking medicines. Drugs may be either inhibitors or substrates for P450s. Some P450s also play a major role in cancer and other diseases. Recombinant P450s offer better activity, …
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Recovery of lactic acid from fermentation broth by electrodialysis
… the productivity of the fermentation and reduce downstream processing costs.
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Pilot-scale process development for the purification of the recombinant antibody 2G12 from transgenic tobacco
… platforms, but this requires the development of downstream processing schemes tailored for both the platform and the product. The aim of this thesis, within the EU-funded project Pharma-Planta, was to develop and optimize downstream processing strategies for the purification of the recombinant …
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A detailed investigation of microbial cell disruption by hydrodynamic cavitation for selective product release
… desirable to reduce the cost involved in their downstream processing. A study of the process variables that affect microbial cell disruption by hydrodynamic cavitation is presented in order to ascertain the conditions required for a selective release. Two model systems were considered (yeast and …
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CFD analysis of reactive crystallisation in stirred tanks : a focus on nickel hydroxide
… basis for their design, frequently resulting in downstream processing difficulties. Previous work by Dustan (2001) considered precipitaion and subsequent dewatering as an integral system, and quantified, to a first order, the relationship between the various sub-processes. The current study is …
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Expression and characterisation of biopharmaceuticals in heterologous expression systems
… conditions for stable antibody production and downstream processing have to be further investigated. For GMP implementation and expression in large-scale reactors under defined conditions protocols and procedures must be established first. Based on the experience in the 100-L downstream …
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Life cycle assessment of dewatering routes for algae-derived biodiesel processes
… is considered a sustainable fuel, but proper downstream processing is necessary to minimize the environmental footprint of this process. Algae is grown in dilute liquid cultures, and achieving the low water contents required for extraction represents one of the greatest challenges for the …
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Dry magnetic assisted impaction mixing of sub-micron boron and barium chromate for a time delay composition
… current Navy wet mixing processes, eliminates downstream processing associated with drying, and shows improved performance in majority of combustion propagation bum tests.
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A generic approach to environmental assessment of microbial bioprocesses through life cycle assessment (LCA)
… relevant constants and physical data. Typically downstream processing units were taken into account and included downstream chemical inputs (reacting or inert). The model was built using a stoichiometric approach, first principles and rules of thumb.
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Magnetically enhanced centrifugation for continuous biopharmaceutical processing
… effective unit operation for biopharmaceutical downstream processing has been demonstrated. Unlike traditional batch-mode HGMS, MEC has a great advantage in that it can be operated continuously as magnetic particles captured on wire surface are constantly removed.
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The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum
… of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial importance such as strain handling and the fatty acid profile were also considered. Eleven fungi in the order Phycomycetes were identified after initial screening. From these fungi, a …
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Gene regulation in baculovirus-infected insect cells : application in the development of a budded virus free expression system
… preparations. However, the cost of downstream processing to remove these particles is considerable. Consequently, this thesis aimed to develop an upstream solution that inhibits BV production, thus eliminating the need for downstream processing. A novel, selectively repressible …
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