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Showing 1 to 20 of 63 for “"Double mutants"”.
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Inherited phenotypic instability of barley homeotic single and double mutants and its possible causes /
… the flower/inflorescence development of barley double mutants Hv-tweaky spike 2; Hooded/Kap1 (Hv-tw2;Hd) that are characterized by inherited phenotypic instability and by several new features, such as leaf-like structures, long naked gaps in the spike, and variations in the basic and ectopic …
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Drosophila Cyclin J And The Somatic Pirna Pathway Cooperate To Regulate Germline Stem Cells
… for oogenesis under normal conditions. Further double mutant analysis of CycJ and neighbor armi, as well as two other piRNA pathway members (piwi and aub), revealed that CycJ genetically interacts with the piRNA pathways by enhancing their ability to regulate oogenesis. Double mutants of CycJ …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
… To test this possibility, we created a number of double mutants lacking both VPS1 and its presumable functional partners. First, we checked the potential growth defects of double mutants and found that double mutants exhibited similar growth rates as single mutant cells. Whether the inactivation …
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The C. elegans class A synthetic multivulva genes inhibit ectopic RAS-mediated vulval development by tightly restricting expression of lin-3 EGF
… resolution, we found that class AB synMuv double mutants exhibit widespread ectopic lin-3 expression. Our results show that lin-3 EGF is normally expressed in the germline, and many class B synMuv genes have previously been implicated in inhibiting germline fates in somatic cells. We …
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Paveldimas miežio viengubų ir dvigubų homeozinių mutantų fenotipo nestabilumas ir galimos jo priežastys /
… the flower/inflorescence development of barley double mutants Hv-tweaky spike 2; Hooded/Kap1 (Hv-tw2;Hd) that are characterized by inherited phenotypic instability and by several new features, such as leaf-like structures, long naked gaps in the spike, and variations in the basic and ectopic …
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Bioenergetic coupling in P-glycoprotein: determining the relative position, topography and role of transmembrane helices six and twelve
… directed mutagenesis was used to generate nine double mutants, each containing one cysteine on TM6 and one on TM12. Double mutants were expressed using the baculovirus/insect cell expression system. Crude membranes containing P-gp were isolated, followed by purification by affinity …
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Bioenergetic coupling in P-glycoprotein: determining the relative position, topography and role of transmembrane helices six and twelve
… directed mutagenesis was used to generate nine double mutants, each containing one cysteine on TM6 and one on TM12. Double mutants were expressed using the baculovirus/insect cell expression system. Crude membranes containing P-gp were isolated, followed by purification by affinity …
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Can plant survive without steroids: Modulation of plant architecture and sex determination through brassinosteroid biosynthesis and signaling
… brassinosteroid biosynthesis and signaling mutants that emphasize the role of BRs in plant growth and development. Two of BR synthetic mutants include brassinosteroid deficient semi-dwarf (bds1) and bds2. The bds1 mutant is a point-nonsense EMS-induced mutation we localized to a gene that …
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Dissection of Regulatory Networks Mediating Resistance and Susceptibility of Arabidopsis thaliana to the Downy Mildew Pathogen Hyaloperonospora parasitica
… signal transduction (PAD4, NDR1, NPR1, RAR1). Double mutant combinations of these signal transduction components were analyzed to detect additive or functionally-redundant contributions to RPP7-dependent resistance. Most of the double mutants support an enhanced level of asexual sporulation …
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Fibronectin and Laminin, extracellular matrix proteins, have an origin in mesoderm tissue and promote anterior neural tube closure in zebrafish
… In contrast, approximately 50% of lamc1 ; fn1a double mutants had an open forebrain neural tube. In the midbrain, some of the embryos deficient in lamc1 ; fn1a had a double roof plate phenotype. In the hindbrain of wildtype embryos, ephrin receptor A4a (ephA4a) is expressed in rhombomeres 1, 3, …
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The self-assembly of proteins: Probing anisotropic protein-protein interactions using phase diagrams
… self-assembly of proteins, we have created three double mutants of human γD-crystallin (an eye lens protein) for which the phase diagrams for the single mutants are known. In effect, these double mutants establish a competition between the influences of each individual amino acid substitution, …
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Terminal uridyl transferases: TUT4/7-mediated RNA metabolism in cancer
… defects in cancer properties in the TUT4/7 double mutants. As the cell type specific differences in defects could be due to the presence or absence of LIN28A, I integrated the LIN28A cDNA in the LIN28A-negative cancer cell line. This allowed the comparison of TUT4/7-dependent gene expression …
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A study of the molecular mechanism of the attenuation response in the threonine operon of Escherichia coli by synthesis of mutant attenuators
… The variants include the wild type; substitution mutants 106C, 136G and 155C; the three corresponding double mutants, 106C/136G, 106C/155C, and 136G/155C; the triple mutant, 106C/136G/155C; and a single-base deletion at position 106 (106$\Delta$). The frameshift mutation removes the leader …
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Transcriptional Regulation of Neuronal Fate Specification by Proneural Genes in the Developing Neocortex
… and neuronal subtypes in Neurog1-/-;Neurog2-/- double mutants (Chapter 4). I found a reduction of both germinal zone and cortical plate size in Neurog1-/-;Neurog2-/- cortices, and I identified severe defects in the radial fibers of Neurog1-/-;Neurog2-/- progenitors. Moreover, I found that deep …
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A genetic analysis of complexin function in neurotransmitter release and synaptic plasticity
… at neuromuscular junctions in complexin null mutants reveals a dramatic increase in spontaneous synaptic vesicle fusion that is independent of nerve stimulation or extracellular calcium. High frequency stimulation at high calcium concentrations shows that the readily releasable pool in …
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Protein Phosphatase 2A Function in Response to Salt Stress in Arabidopsis thaliana: Defining Pathways and Genetic Interaction Networks
… phenotypes. A subset of the A and C subunit mutants exhibited root skewing or curling phenotypes when grown on vertically-oriented plates supplemented NaCl. I characterized the root response of a1rcn1 and c4 mutants and found that the root phenotype was sodium specific and influenced by NaCl …
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Hierarchy of Interactions in Protein Evolution
… scanning to analyze nearly 50,000 single and double mutations in several homologs of a model protein - the PDZ family of protein interaction domains. Across the domains queried experimentally, the distributions of couplings between pairs of positions from all possible double mutants are …
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The role of SYN1 in early Arabidopsis meiosis
… fragmentation in the syn1\(^{-/-}\), three double mutants were constructed. I have demonstrated that chromosome fragmentation in syn1 is AtSPO11-1-dependent. Moreover, I have also shown that SYN1 has a role in DSB repair by analysing Atdmc1\(^{-/-}\)/syn1\(^{-/-}\) meiocytes. To investigate …
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Regulation of Neuronal Polarity by Wnt Signaling
… its involvement in this process. cfz-2/fz;lin-17 double mutants were found to enhance the ALN cell fate defects observed in lin-17 mutants. Finally the transgenic lines expressing PLR-1 in the mechanosensory neurons were crossed with cam-1 mutants enhancing the ALM defects in one line, and with …
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Lst1p and Exp1p act in parallel pathways to export the plasma membrane H⁺-ATPase from the ER in S. cerevisiae
… of the lethality displayed by Ist1-] sec13-1 double mutants. Expression of EXP1 from a centromeric plasmid suppresses the sensitivity of IstlA strains to growth on acidic medium and restores plasma membrane localization of Pma1p. Unlike stlA strains, exp1 strains grow normally under acidic …
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