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Showing 1 to 20 of 29 for “"DnaK"”.
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Biochemical characterizations of Escherichia coli DnaK and DnaK mutant proteins purified from ndk deficient cells
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
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Inhibitory effects of Green synthesized Senna alexandrina silver nanoparticles (SAAgNPs) on Escherichia coli DnaK
… structural integrity of the heat shock protein DnaK. The research presents an improved synthesis process for silver nanoparticles (AgNPs) from the Senna alexandrina leaf extract. In addition to evaluating the synthesized AgNP's physicochemical characteristics, the research also examines their …
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In vitro Analysen der Interaktion von Chaperonen mit naszierenden Polypeptidketten
… Trigger Faktor (TF) und das Hsp70-Chaperon DnaK an der Faltung neusynthetisierter Proteine beteiligt. In der vorliegenden Arbeit wurde die kotranslationale Interaktion von TF und DnaK mit naszierenden Polypeptidketten untersucht. Dazu wurde ein zellfreies Transkription-Translationssystem auf …
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In cellulo and biophysical exploration of the role of GGMP residues of Hsp70
… the GGMP residues of Hsp70s using E. coli Hsp70 (DnaK) and chimeric protein, KPf as models. Chimeric protein KPf is made up of NBD of DnaK and SBD of PfHsp70-1. E. coli DnaK lacks the GGMP residues that are present in PfHsp70-1. DnaK-G (DnaK modified to include GGMP residues) was created to …
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Purification and Activity of the DnaK Heat Shock Protein of the Emerging Human Pathogen Rhodococcus equi. Optimisation of methods of purifying DnaK from Rhodococcus equi, and the use of the purified protein in assays to demonstrate its activity in isolation and with other heat shock proteins
… methods to purify the heat shock protein DnaK from R. equi, using a combination of ion-exchange and affinity chromatography. The effectiveness of the purification protocols were assessed using SDS-PAGE and Western-blotting with anti-DnaK antibodies, and the enzymic activity of the purified …
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Purification and Activity of the DnaK Heat Shock Protein of the Emerging Human Pathogen Rhodococcus equi. Optimisation of methods of purifying DnaK from Rhodococcus equi, and the use of the purified protein in assays to demonstrate its activity in isolation and with other heat shock proteins
… methods to purify the heat shock protein DnaK from R. equi, using a combination of ion-exchange and affinity chromatography. The effectiveness of the purification protocols were assessed using SDS-PAGE and Western-blotting with anti-DnaK antibodies, and the enzymic activity of the purified …
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Stress response of porphyromonas gingivalis
… proteins were identified as homologs of E. coli DnaK and GroEL respectively by Western immunoblotting. On two-dimensional Western blots, two forms of DnaK and four forms of GroEL were identified due to their slightly different isoelectric points. dnaK and groEL gene homologs were identified in …
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Comparative analysis of a chimeric Hsp70 of E. coli and Plasmodium falciparum origin relative to its wild type forms
… constitutively. The Hsp70 family also known as DnaK in Escherichia coli (E. coli) is the most well-known group of molecular chaperones. Structurally, Hsp70s consist of a nucleotide binding domain (NBD) and a substrate binding domain (SBD) conjugated by a linker sub-domain. ATP binding and …
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Interaktionen von HLA-DR-Molekülen mit Chaperonen der HSP70-Familie und deren Bedeutung für das Prozessieren und Präsentieren von Autoantigenen
… der a-Kette Chaperone aus E. coli, nämlich DnaK (HSP70) und in geringen Mengen DnaJ (HSP40), enthielt. Durch Depletion an ATP-Agarose konnte DnaK entfernt werden. Die so gewonnene DnaK-freie Antigenpräparation wurde zur Stimulation des humanen T-Zell-Klons PM-A C3 eingesetzt. Im Gegensatz zu …
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Development of high-throughput screening assays to identify small molecule inhibitors of Plasmodium falciparum Hsp70-1
… using the complementation assay. The E. coli dnaK756 mutant strain can grow between 30- 37 ℃ but its viability diminishes at temperatures >40 ℃. In addition, the strain produces non-functional Hsp70. As such, this strain is suitable for studying Hsp70 function using the complementation assay. …
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Investigation of the role of the GGMP motif of Plasmodium falciparum Hsp70-1 on the chaperone function of the protein and its interaction with a co-chaperone, PfHop
… substituted were generated. E. coli Hsp70 (DnaK) lacks a GGMP motif. Thus, the GGMP motif of PfHsp70-1 was introduced into E. coli DnaK in order to generate a third GGMP variant. Recombinant forms of PfHsp70-1, DnaK, and their GGMP variants were heterologously expressed in E. coli XL1 Blue …
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Pandemic <em>Vibrio parahaemolyticus</em>: Defining Strains Using Molecular Typing and a Growth Advantage at Lower Temperatures
… three proteins (DnaA, DnaJ-related protein and DnaK-related protein) with functions related to cold stress. DnaA was expressed in the non-pandemic strain and not the pandemic strain, while the reverse was true for DnaJ-related and DnaK-related proteins. Western blot analysis and LC-MS/MS …
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Evaluation of the effects of gold nanoparticles (AuNPs) on protein folding in Escherichia coli
… with minimal effects on cellular integrity in DnaK- cells. DnaK + cells exhibited containment of AuNPs in the cytosol. AuNPs also inhibited E.coli growth marginally and had no observable effect on the solubility of E.coli proteins at the concentrations tested (25-75 μg/mL) in DnaK+ cells. MDH …
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Elucidation of the role of the linker motifs of Plasmodium falciparum Hsp70-1 and Hsp70-z
… which closely resembles Escherichia coli Hsp70, DnaK. On the other hand, PfHsp70-z is a non-canonical Hsp70 which belongs to the Hsp110 subfamily. Hsp70s exhibit a highly conserved structural architecture characterized by an N-terminal nucleotide-binding domain (NBD) and a C-terminal …
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Effects of co-expression of chaperone combinations on production of soluble plasmodial protein PfAdoMetDC in E. coli
… combinations: combination 1 (pBB535) encoding DnaK with DnaJ; combination 2 (pBB535-Pf70J) encoding PfHsp70 with DnaJ; combination 3 (pBB535-KPfJ) encoding KPf with DnaJ; combination 4 (pBB542) encoding DnaK with DnaJ and GroEL; combination 5 (pBB542-Pf70JE) encoding PfHsp70 with DnaJ and …
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Charakterisierung modularer Peptidyl-Prolyl-cis-trans-Isomerasen
… an Trigger-Faktor binden können. Anders als DnaK bindet Trigger-Faktor seine Substrate dynamisch und nukleotidunabhängig. Dies dürfte für eine hohe katalytische Aktivität von Vorteil sein, nicht jedoch für eine möglichst effiziente Chaperonwirkung. Die synthetische Letalität, die auftritt, …
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Effect of Starter Cultures on Lactobacillus acidophilus Survival and Gene Expression in Yogurt
… gene expression of selected stress genes (dnaK and groEL) was determined using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) method. This preliminary study demonstrated the important of dnaK in L. acidophilus NCFM to maintain its viability under various stress …
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Protection of Artemia from vibriosis by heat shock and heat shock proteins
… heat shock proteins, particularly the 70kDa DnaK, promotes resistance to infection by a pathogenic bacterium. This study demonstrates for the first time that Hsp70 homologs can induce a protective effect towards a pathogen when it is supplied via the feed to the host, perhaps via immune …
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Analyse des Hitzeschocks bei Neisseria meningitidis mit DNA-Microarrays
… dieser Gene, darunter groEL/groES und dnaJ/dnaK, war bereits bei anderen Organismen beschrieben worden, was die Qualität und Reproduzierbarkeit der Ergebnisse unterstreicht. Es konnte gezeigt werden, dass die Intensität des Hitzeschocks und damit die Deregulation der Hitzeschock-Gene mit …
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Further characterisation of the envelope stress responses of Salmonella Typhimurium
… of the virulence factors MntH and GroEL, GroSL, DnaK and ClpB (the heat shock chaperones). In addition, we show critical requirement of ZraSR for maximal carbon-starvation induced cross-resistance to heat and polymyxin B. The ZraR regulon described here includes functional groups required to …
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