Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Dithiothreitol (DTT)"”.
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Development and application of electrochemical dithiothreitol (DTT) assay for analysis of particulate matter
… an electrochemical version of the well-studied dithiothreitol (DTT) absorbance spectroscopy assay is presented as a platform for the analysis of PM in air samples. Flow injection amperometry was used as the primary electrochemical method. Amperometry was performed using a CH Instruments …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
… retained in the presence of reducing agent, 2 mM dithiothreitol (DTT). Moreover, DNA-hydrolyzing activity of 3D8 scFv was maintained after lyophilization and 30 cycles of freeze-thaw. When the 3D8 scFv was stored in pH 3-12 for a month at 37°C, DNA-hydrolyzing activity was retained at pH 4-8. In …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… in the pH 4-6 region. Addition of 50 mM dithiothreitol (DTT) to the samples prior to isoelectric focusing (IEF) and an increase of IPG buffer concentration greatly improved spot resolution. Silver staining achieved higher spot counts than Coomassie staining. The influence of the number of …
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Impact of heated passive headspace concentration on DNA recovery from semen in fire-scene evidence
… HPHC at 90 °C for 6 h or 16 h. The influences of dithiothreitol (DTT) pretreatment and gasoline contamination were also assessed. DNA yield, degradation index (DI) and short tandem repeat (STR) profiles served as outcome measures.When not contaminated with gasoline, about 60 ng of DNA (DI≈1.6) …
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The Isolation and Characterization of Growth Regulatory Factors Produced by a Herpes Simplex Virus Type 2 Transformed Mouse Tumor Cell Line, H238
… trypsin, neuroaminidase, and the reducing agent dithiothreitol (DTT) and, resembled a platelet-derived growth factor (PDGF)-like factor; and the third displaced [ 125I]-labeled basic fibroblast growth factor (bFGF) in a dose dependent fashion when tested with a radioimmune assay (RIA). The fourth …
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Regioselective Synthesis of Glycosaminoglycan Analogs
… to amines using a novel reducing reagent, dithiothreitol (DTT). Alternatively, another process utilized thioacetic acid to reduce azides to a mixture of amine and acetamido groups. Through pursuing these GAG analogs, we applied novel azide reductions by DTT and thioacetic acid that are new …
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Investigating the mechanical properties of yeast cells
… properties of treating Baker’s yeast with dithiothreitol (DTT) was investigated. DTT has the effect on Baker’s yeast cells of breaking the disulphide bonds in the cell wall releasing invertase into the suspending solution. It was found that this did not affect the intrinsic material …
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Rethinking the dithiothreitol based PM oxidative potential: measuring DTT consumption versus ROS generation
… a specific ROS, hydroxyl radical (•OH) in dithiothreitol (DTT) assay. DTT assay has been a widely-used probe to measure the oxidative potential as it measures the consumption rate of DTT which presumably corresponds to the generation rate of a specific ROS - superoxide radicals (O2•-). …
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Role of extracellular environment in mechanical properties of human cardiac fibroblasts and myofibroblasts
… of 20 kDa 8-arm PEG-VS hydrogel crosslinked with dithiothreitol (DTT) reagent, is directly proportional to the concentration of PEG precursor used to form the material. Rheological measurements indicated an elastic shear modulus of 500 ± 13 Pa and 2721 ± 39 Pa for PEG gels of 4% (m/v) and 10% …
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The effect of reduction, trypsin digestion and de-glycosylation of salivary mucins in the inhibition of human immunodeficiency virus type 1
… rich fractions were either reduced with 10mM dithiothreitol (DTT) or proteolytically digested with 0.25% trypsin. The resultant fractions were dialysed and freeze dried. Slot blots were used to determine the identity of the void volume (Vo) fractions and the included volume (Vi) fractions …
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Elucidating the Role of Toxin-Induced Microbial Stress Responses in Biological Wastewater Treatment Process Upset
… NEM and CDNB, and the thiol reducing agent, dithiothreitol (DTT). The polyether ionophore antibiotic, nigericin, was used to artificially stimulate K+ efflux from S. capsulata and activated sludge cultures. Thus, glutathione-gated K+ efflux (GGKE) activity may cause K+ release from the …