Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Disulphide bonds"”.
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Understanding the biochemical properties and physiological function of the protein syncollin
… neutrophils. The protein contains intramolecular disulphide bonds and is present both free within the ZG lumen and tightly associated with the luminal leaflet of the ZG membrane; it is also secreted into the pancreatic juice. Syncollin is able to oligomerize, and assemble into doughnut-shaped …
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Discovery, bioactivity evaluation and rational design of natural-occurring peptides from Agalychnis annae and Rana tagoi okiensis
… its truncated form, QUB-1228.5, with the middle disulphide bonds and their respective non-oxidized analogues, QUB-2490 and QUB-1230.5, were studied to explore their bioactive functions. These peptides showed varying degrees of myotropic effects on rat bladder smooth muscle relaxation which might …
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Midkine - a host defence protein
… anti-parallel β-sheets in each domain and five disulphide bonds stabilize the structure. We found that MK has both antibacterial as well as antifungal properties and exert these activities by disrupting the membranes of the microorganisms, resulting in leakage of intracellular contents. The …
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Structural determinants of stability to proteolysis, processing and impact on allergenic potential of non-specific lipid transfer proteins
… proteins comprising four intramolecular disulphide bonds making the structure very resistant to proteolysis and harsh food processing conditions. These proteins are identified as strong allergens sensitizing through the gut and share epitopes with LTPs from closely related species. Peach …
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Structural studies on the iron storage protein, apoferritin
… band could be attributed to inter-molecular disulphide bonds forming dimers and trimers and an intra-molecular disulphide bond giving an apparently smaller subunit band (m.wt. 15,000). Other smaller molecular weight bands have been re-assessed by SDS-PAGE to have molecular weights of 11,000 …
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Investigating the mechanical properties of yeast cells
… effect on Baker’s yeast cells of breaking the disulphide bonds in the cell wall releasing invertase into the suspending solution. It was found that this did not affect the intrinsic material properties or failure criteria. In addition to this, Baker’s yeast cells were mechanically perturbed by …
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Studies on the natural fluorescence of wool and wool grease
… level of fluorescence was observed when the disulphide bonds of cystine were oxidised to cysteic acid residues, due to the removal of the quenching disulphide bond. Each of the morphological components examined (cuticle, cortex and cell membrane complex) were fluorescent. Chemical bleaching …
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Single-Payload Bioconjugation to Native Antibodies And A General Method for the Self-Immolative Release of Amide-Containing Molecules
… conjugates by re-bridging all four interchain disulphide bonds in IgG1 antibodies with a single construct. Unlike earlier generations, the TetraDVPs used in this work contain the payload before the bioconjugation occurs; this means the entire construct is added in a single step ‘all-at-once’, …
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Characterisation of marama bean protein
… were similar, suggesting an absence of disulphide bonds. The vicilin (7S) and acidic 11S subunits seemed to be absent in marama. This is most unusual in legume proteins. Only a major basic legumin (11S) (20 kDa), medium (63 kDa) and high (148 kDa) molecular weight protein bands were …
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Characterisation of quiescin-sulfhydryl oxidase and nematode astacin mutants using functional studies in caenorhabditis elegans.
… the generation of a flexible yet robust cuticle. Disulphide bonding is crucial in the formation of the cuticle, with cysteine cross-linking mutants having been shown to produce severely disrupted cuticles and associated lethal phenotypes. During the life cycle, C. elegans progresses through four …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… highly purified protease. The presence of four disulphide bonds per molecule of the enzyme was indicated with dithionitro-benzoate. No free sulphydryl groups were found. The purified protease contained approximately 10% carbohydrate. The amino acid composition of Caldolase was determined. The …
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An investigation into novel bacteriocin producers isolated for shelf life extension of dairy products
… natural actifensin and lacked highly conserved disulphide bonds. Actinomyces oris CCUG 34286 containing seven afnA copies did not exhibit characteristic actifensin activity. As defensin-like structures are conserved throughout nature, other actifensin-like operons were sought using gene …
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Optimierung von Schizosaccharomyces pombe für die heterologe Genexpression
Die vorliegende Arbeit beschäftigt sich mit der genetischen Optimierung der Spalthefe S. pombe für die biotechnologische Produktion von Fremdproteinen. Hierbei werden vor allem zwei Aspekte näher untersucht, zum einen die Stabilität des zu produzierenden Proteins und zum anderen die Bildung von …
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Interactions of viral and cellular Tumour Necrosis Factor Receptor molecules
… critical amino acids such as cysteines in disulphide bonds, display reduced TNFR-induced cell death as determined by flow cytometry. To better understand the biology of the vTNFR association with cellular TNFRs, and with WHO Smallpox committee approval, the human tropic poxviral TNFRs from …
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An extracellular protease from an extreme thermophile
… Daltons and 8.5, respectively. The presence of 6 disulphide bonds was indicated by the reaction with dithionitro-benzoate. No free sulphydryl groups were detected. When tested against a wide range of inhibitors, significant responses to EDTA, EGTA, and iodoacetic acid, but not o-phenanthroline or …
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Evaluating the impact of ultraviolet light on chemical and mechanical properties of human scalp hair through controlled and natural experiments
… region) and had the lowest absorption for the disulphide bonds. The TGA/DTG showed that the onset degradation temperature (ODT) of UVB-treated fibres was significantly reduced compared to both the control group and UVA exposed group. The MTT results showed increased yield stress and yield …