Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Dissociation Kinetics"”.
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Kinetic Analysis of High Affinity Polyclonal Anti-Fluorescyl Immunoglobulin-G Antibodies
… measurement and assessment of association and dissociation kinetics in anti-fluorescyl antibodies were developed in these studies. An analytic procedure for the characterization of heterogeneous first order kinetics was derived. This procedure was based on Laplace transformation of the gamma …
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Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
… more than 1000-fold with respect to ligand dissociation kinetics and equilibrium binding affinity have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring binding improvements suggests a potentially …
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A Fractal Analysis of the Detection of Glucose Molecules on Biosensor Surfaces
A fractal analysis for the binding and dissociation of glucose molecules to different biosensor surfaces is presented. The sensors contain immobilized glucose oxidase enzymes that have an affinity for the glucose molecules in solution. Data for different modified biosensors is modeled and analyzed, …
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The Effect of Particle Surface Area to Volume Ratio on Ion Release from CoCr Spheres
… An improved comprehension of the corrosion kinetics and the rate of ion production from the high surface energy wear debris released by implant articulation can assist in illustrating the relative clinical significance of exposure to these metallic bodies over time. This thesis primarily …
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Self-assembled peptide as a long-acting drug formulation
… peptides, and allow a controlled release by dissociation of monomers from the fibril termini [5]. Here I propose self-assembled peptides as a prolonged-activity, self-mediated drug delivery system for subcutaneous injection of pharmaceutically active drugs. Oxyntomodulin is used as a model …
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Mechanistic and Engineering Studies of LOV Photoreceptors in Yeast
… vivo </em>fluorescence characterization, and kinetics in conjunction with <em>in vitro </em>classifications. Using flow cytometry-based transcriptional activity assays of 23 EL222 mutants in the budding yeast <em>Saccharomyces cerevisiae </em>produced a dynamic range of expression profiles. A …
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Ruthenium K-edge X-ray absorption spectroscopy studies of ruthenium complexes relevant to olefin metathesis
… for the unexplained differences in phosphine dissociation kinetics for complexes 1 and 2: the phosphine dissociation rate of 2 is slower than 1 because the metal centre is more electron-deficient in 2. Density functional theory calculations confirm the charge differences and offer some insight …
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Characterization of platelet glycoprotein Ib-IX-V: von Willebrand factor interaction under shear conditions
… In the first part of the work, we evaluated the kinetics of interaction between platelet GP Ib-IX-V complex and VWF under arterial flow conditions. The GP Ibalpha subunit of GP Ib complex binds to VWF through the Al domain of VWF. Impaired GP Ib-VWF interaction due to GP Ibalpha mutations can …
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The Choreography of Base Excision Repair
… APE1 to Pol β during BER is dependent on the dissociation kinetics of APE1 and the duration that Pol β remains bound near the APE1-5’ nick complex. Altogether, our work provides insight into the intermolecular interactions between APE1, Pol β, and DNA which facilitate the coordination of DNA …
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Functional Relevance of Protein Disorder: Why is Disorder Favourable?
… system: phosphorylation modulates only the dissociation kinetics increasing the lifetime of the bound complex, which may be important in signalling processes. Further, phi-value analysis applied to investigate the mechanism of interaction reveals that non-native interactions play a key role …
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Studies of CD36 interacting with fatty acids, oxidized low-density lipoprotein, and the cellular plasma membrane
… channels, generating real-time association and dissociation binding curves. With the exception of the oxidized FA hydroxyoctadecadienoic acid (HODE), all FA tested bound to CD36 with rapid association and dissociation kinetics similar to human serum albumin. In addition, FA increased oxLDL …
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Very accurate gas-phase thermochemistry through photoelectron photoion coincidence (PEPICO) spectroscopy
… Source, which is capable to determine ionic dissociation energies to sub-kJ/mol accuracy. The iPEPICO on the synchrotron beamline was also useful where measurements required energies in excess of 14 eV. The modeling framework of PEPICO is based on the RRKM statistical theory of dissociation …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
… assay of a PNS after different chase times, HRP dissociation from membrane was observed over a 30 minute chase period. Internalized HRP in the presence of yeast mannan (19 mg/ml), intravesicular pH elevators HEPES (40 mM) or monensin (10 μM), or substances which should deplete cellular ATP …
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An Investigation into the pharmacology of the ghrelin receptor
… Radioligand binding experiments showed that the dissociation kinetics of [His[125I]]- ghrelin from the GRLN-R were not altered by co-administration of the growth hormone secretagogues. Fitting data to a modified operational model of allosterism demonstrated that GHRP-6, L-692,585 and MK-677 were …
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Machine learning and uncertainty quantification framework for predictive ab initio Hypersonics
… of the physics governing the non-equilibrium dissociation of O2 molecules colliding with O atoms is proposed. A rovibrationally-resolved database for all of the elementary collisional processes is constructed by including all nine adiabatic electronic states of O3 in the QCT calculations. A …
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Structure and Dynamics of Associative Polymer Gels
… hopping was shown to be important only in kinetics-limited systems, where the sticker association/dissociation dynamics are slower than the intrinsic Rouse relaxation of the network strands. The simulations were also used to probe the effects of molecular parameters such as the sticker …
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Interactions between nucleoid associated proteins and DNA in the presence of mechanical and chemical forces
… how their physical properties (such as binding kinetics or DNA manipulation) aid in regulating cell function. It has been shown in experiment and simulation that NAPs can adopt multiple binding states (i.e., the protein can be partially associated with its DNA substrate), which leads to complex …