Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Directed differentiation"”.
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Directed differentiation of endodermal cells from mouse embryonic stem cells
Pluripotent embryonic stem cells hold a great promise as an unlimited source of tissue for treatment of chronic diseases such as Type 1 diabetes and chronic liver disease. Various attempts have been made to produce cells that can serve as precursors for pancreas and liver. By using …
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
… were studied by ectopic expression. Then we took directed melanocyte differentiation as a model to enrich transgenic differentiating cells. For this, a bicistronic plasmid was constructed that enables the transgenic medaka embryonic stem cell to differentiate into melanocyte and confer drug …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… are epigenetic regulators necessary for proper differentiation of cells and for mammalian development. Notably, faulty regulation of PRC2 has been associated with a broad range of cancers, suggesting that it has a critical role in maintaining cell identity. Polycomb Repressive Complex 2 (PRC2) …
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The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
… enter a primed, epiblast-like state of early differentiation when microfluidic perfusion is used to deplete soluble cell-secreted signals. We show that this phenotypic change can be used to our advantage for directed differentiation, and further demonstrate that remodeling the endogenous …
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LONG NON-CODING RNA VENTHEART IS NECESSARY FOR VENTRICULAR CARDIOMYOCYTE SPECIFICATION AND FUNCTION
… (hPSC-CM) as an in vitro model for cardiac differentiation and development, VHRT was found to be highly co-regulated with crucial cardiac and ventricular-specific genes. CRISPR deletion of VHRT locus revealed that VHRT was essential for maintaining the cardiac gene expression profile, …
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A microfluidic platform for three-dimensional neuron culture
… factors that control, direct, and enhance differentiation, axonal projection and connectivity. While techniques are available to separate specific populations of neurons once they are fully-differentiated, current methods make it nearly impossible to monitor or control the development of a …
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An improved method for generating oligodendrocyte progenitor cells from murine induced pluripotent stem cells
… therapeutic applications. It can be generated by directed differentiation of a variety of pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells. The iPS cell is an ideal source for derivation of OPCs, as it offers the advantage of autologous transplants, and a …
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A Stem Cell-Based Strategy for Modeling Human Kidney Disease and Discovering Novel Therapeutics
… achieve this goal, we leveraged advances in the directed differentiation of stem cells and in vitro disease modeling techniques to develop translationally relevant human models of podocyte injury. We used these models to identify potential biomarkers of early onset podocyte dysfunction, …
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Scalable production of cellular aggregates for the differentiation of embryonic stem cells into cardiac muscle
… However aggregation, an important aspect for the directed differentiation of ES cells, only occurred using the "floating drop" method, and this method was used to encapsulate a predetermined number of cells in capsules of a specified size. The "floating drop" method has the advantage that culture …
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An omics study into the molecular impact of autosomal dominant APP and MAPT mutations on the cerebral cortex
… The first half of this thesis focuses on the differentiation of patient-derived iPSCs carrying MAPT E10+16 and APP duplication mutations and isogenic control iPSCs into neuronal cultures using the directed differentiation protocol. I first describe the CRISPR/Cas9 strategy to correct APP copy …
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Extended transient transfection by repeated delivery of in vitro-transcribed RNA
… become a powerful tool in the development of new directed-differentiation and cell-type-conversion protocols.
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Engineering Tools to Promote and Characterize Wnt-Mediated Stem Cell Differentiation
… luciferase secreted by these cell lines during directed differentiation into cardiomyocytes permit continuous non-destructive monitoring of the manufacturing process. These cell lines could potentially guide process optimization and enable production of cardiomyocytes with a more mature …
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Molecular Consequences of High Taz Expression In Gliomas
… the cell, with consequent silencing of neuronal differentiation factors and an activation of mesenchymal regulators. Directed differentiation of glioma stem cells towards the post-mitotic neuronal state have been shown to be efficient strategies to battle this disease. By establishing the role of …
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Characterization of the Effects of bFGF and GSK3β Inhibitors on Embryoid Bodies Derived from Human Pluripotent Stem Cells
… step in many human pluripotent stem cell (hPSC) differentiation protocols. Previous work suggests that EBs are sensitive to growth factor withdrawal if they are derived from hPSCs maintained in feeder independent media such as mTeSR1. To promote cell survival, EBs generated from mTeSR1-adapted …
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Elucidating the Role of Cellular Architecture in the Developing Pancreas
… these cells in vitro for replacement therapies. Directed differentiation protocols have improved upon transitioning from 2D to 3D cultures, indicating that the 3D microenvironment in which β cells are born is critical for the acquisition of their cell fate. However, little is known about the …
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Investigation of pancreatic beta cell differentiation and function, and global profiling analysis of diabetes serum for the identification of disease biomarkers
… reprogrammed iPS cells were not identified. Directed differentiation of an established iPS cell line was performed in 2D and 3D culture, with improved efficiency of definitive endoderm formation in 3D cultures (a step on the differentiation routetowards beta cells). In parallel, serum from …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
… embryonic stem cell (ESC) pluripotency and differentiation. This may be a source of heterogeneity resulting in mixtures of cell types in differentiated populations despite efforts to control the differentiation conditions and the use of a clonal starting population. In addition, this …
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Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells
… required for epigenetic silencing of Oct4 during differentiation. The goal of this thesis was to examine the role of CDK2AP1 in somatic cells (primary human dermal fibroblasts (HDFs)) and human embryonic stem cells (hESCs) and specifically assess its impact on proliferation, self-renewal and …
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Artificial neural network and precision genome engineering frameworks for genetic system engineering in mammalian cells
… using GATA6-mediated and Neurogenin-mediated directed differentiation. The sensor provides a fluorescent readout based on the activity of a specific miRNA with single-cell resolution, and has applications in organoid engineering and basic research. The machine learning frameworks are comprised …
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Genomic profiling and perturbation of Hepatitis B virus infection
… cure. First, we use micro-patterning tools and directed differentiation protocols to develop primary adult human hepatocyte and iPS-derived hepatocyte models of HBV infection, respectively, which enable us to test antiviral therapies with multiple mechanisms of action and identify a significant …
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