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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Direct PCR"”.
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Evaluation of direct PCR for forensic DNA profiling and the development of a direct PCR multiplex
The use of direct PCR with different types of sample was explored in this study. Genomic DNA preparations at various concentrations and buccal cell counts were deposited on commonly encountered substrates, recovered and amplified using direct PCR before subjecting them to capillary electrophoresis. …
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A Comprehensive Evaluation of a new Direct Amplification System (PowerPlex® 18D) in Forensic DNA Profiling
… DNA profiles in a fast and reliable manner. Direct amplification, also referred to as direct PCR, is one alternative method that has been proposed to address this issue. Direct amplification allows for the generating of DNA profiles without using the DNA isolation process. While direct PCR …
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The molecular systematics of Southern African Testudinidae
… mtDNA cytochrome b sequence were obtained, using direct PCR-based sequencing, from 32 individual tortoise blood samples, comprising 13 different species from 6 genera. PAUP 3. 1.1, and MEGA were used to infer a phylogeny using Chrysemys scripta elegans (an Emydid) an outgroup. Both phenetic and …
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Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit
… first time success rate was observed using the direct PCR approach. Allele frequencies were significantly different between all four population groups at three markers (D8S1179, D2S1338 and D2S441) after a Bonferroni correction (ρ < 0.001) and sixteen novel alleles were observed. Two genetic …
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Historical Genetic Analysis Of Horned Lark (Eremophila Alpestris) Specimens From The Prairie Pothole Region
… chelating resin with dithiothreitol, and a Direct PCR method without DNA purification. Three fragments of mitochondrial DNA loci were amplified including control region, cytochrome c oxidase I and NADH dehydrogenase subunit 2. Correct sequencing results indicated that this method is viable …
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Binding and Signaling Differences between Prostaglandin E1 and E2 Mediated by Prostaglandin E Subtype Receptors
… The results were used as a guide for site-directed mutagenesis of the hEP3 protein. The S211L and R214L mutants expressed in HEK293 cells lost binding to [3H]PGE2. This study found that the non-conserved S211 and R214 of the hEP3 are involved in PGE2 recognition. The mutant S211L was able …
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Investigation into implementing a massively parallel sequencing workflow for forensic human identification in South Africa
… kit. This entailed systematic adjustments to a direct PCR approach and the development of a lysate purification method. This optimised approach was subsequently used to conduct a population study comprising 463 consenting South African volunteers, wherein the first sequence-based allele …
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Investigation into implementing a massively parallel sequencing workflow for forensic human identification in South Africa
… kit. This entailed systematic adjustments to a direct PCR approach and the development of a lysate purification method. This optimised approach was subsequently used to conduct a population study comprising 463 consenting South African volunteers, wherein the first sequence-based allele …
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DNA profiling of single sperm cells and single skin flakes in forensics using micromanipulation and whole genome amplification.
… profile could ideally be overcome. However, PCR is not a sensitive enough method to generate DNA profiles by STRs on single cells. Moreover, high quality intact DNA is required, but is not always available in skin flakes due to degradation. Additionally, single skin flakes are difficult to …
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In vitro Expression of Human Zona Pellucida Protein 3
… transcription-polymerase chain reaction (RT-PCR). The hZP3 cDNA was subcloned into PSK and pREP4 expression vectors. The cDNA of hZP3 was further characterized by restriction mapping, PCR, auto-sequencing and Southern blot analysis by using an internal oligonucleotide probe, and found to be …
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A Novel Forensic DNA Profiling Method Based On Molecular Beacons Without Dna Purification
<p>Analysis of polymorphisms in nucleic acid sequences provides the basis for identification of individuals and their genetic deficiencies. Currently, the accepted method of analysis for profiling is Short Tandem Repeat (STR) profiling. This is a lengthy process, typically taking up to 3 days. The …
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Systematics and Ecology of Truffles (Tuber)
… of Tuber melanosporum. The method is based on direct PCR and species-specific primers and is very useful for rapid diagnostics. I have adapted this approach for other truffle and mushroom species. </p><p>Three major findings emerge from my dissertation research: 1) Tuber is more diverse than …
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Strategies for Enhanced Genetic Analysis of Trace DNA from Touch DNA Evidence and Household Dust
… a micro-manipulation and combined cell lysis/direct PCR amplification technique has been developed that is sensitive enough to obtain full or probative STR profiles from single or clumped bio-particles collected from touch DNA and dust evidence. Sources of touch DNA evidence such as worn …