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Showing 1 to 11 of 11 for “"Dipicolinic acid"”.
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Statistical Considerations in Designing for Biomarker Detection
… particular interest is 2,6-pyridinedicarboxylic acid (dipicolinic acid, or DPA), a molecule that is present at high concentrations in spores of Clostridium and Bacillus, the latter of which includes the threat organism Bacillus anthracis, or anthrax. Dipicolinic acid may be useful as a first-step …
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A Role of Intracellular Polysaccharide in the Sporulation of "Bacillus Cereus"
… the ability to accumulate poly-B-hydroxybutyric acid and intracellular polysaccharide followed by comparison to wild type levels. Endotrophic sporulation capabilities with exogenous glucose were determined by the heat resistance of spores in both mutant and wild type. Dipicolinic acid levels in …
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Analysis of Peptidoglycan Structural Changes and Cortex Lytic Enzymes during Germination of<i> Bacillus anthracis</i> Spores
… spores of three species release the spore solute dipicolinic acid and resume metabolism at similar rates, the <i>B. anthracis </i> spores released 75% their cortex material within 10 minutes while the other species released only 20% in the same time frame. This suggests that the <i>B. …
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Spore-forming bacteria as pharmaceutical factories
… better drug products? Key spore component 2,6-dipicolinic acid (DPA) is necessary for protein stability, but investigation of its role is confounded with that of water content. Tests replacing DPA with chemical analogues were not successful, but led to the novel approach of doping spores with …
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Generation of Biomarkers from Anthrax Spores by Catalysis and Analytical Pyrolysis
… for this include platinum, nickel, and superacids. Experiments evaluating several of these materials are described. A biomarker mass spectral library was created, based on information available in the scientific literature, to facilitate analysis and identification of the biomarkers produced …
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The Roles of the Germination-Specific Lytic Enzymes CwlJ1, CwlJ2, and SleB in Bacillus anthracis Spores
… it participates in germination in response to Ca-Dipicolinic acid; a role it shares with the more dominant CwlJ1. Purification of the CwlJ1 and SleB proteins permitted in vitro assays of enzymatic activity as measured by changes in substrate optical density, solubility, and product formation. …
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Survival strategies of Mycobacterium avium subsp. paratuberculosis in a variety of microenvironments.
… represent a group of closely related acid-fast bacilli that encompass a wide-range of host tropisms and diseases. Mycobacteria can be divided into two complexes: the Mycobacterium tuberculosis complex and the Mycobacterium avium complex (MAC). The MAC is comprised of M. avium subsp. …
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Determination of actinide elements in environmental samples by ICP-MS
… coated with permanently bonded iminodiacetic acid. The latter was used for the successful determination of uranium and thorium in certified reference material waters. However, the column was found to have a high affinity for iron, making it unsuitable for the determination of the actinides in …
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Factors Affecting the Heat Resistance of Clostridium perfringens Spores
… observations drawn from our wild-type strains. Dipicolinic acid is a major component of bacterial spores and is necessary for spore heat resistance. The Cluster I clostridia, including C. perfringens, lack the known DPA synthase operon, spoVF. We developed an in vitro assay for detecting DPA …
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Drinking Water Quality Monitoring
… the cellular components excited include calcium dipicolinic acid (DPA) for spores. By using these three different wavelengths, the prototype can differentiate between viable and non-viable organisms and also has the potential to detect spores. The aim of this study was to improve the detection …