Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Difficult-to-express"”.
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Investigating "difficult-to-express" monoclonal antibody frameworks in transient and site-specific integration-based Chinese hamster ovary cell expression systems
… set of characteristics that defines its ability to be manufactured at large-scale using current practices in the biopharmaceutical industry. Failure to identify low developability mAb candidates prior to process development can necessitate significant time and resource investments to mitigate …
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Dirty Laundry
… for me. Working in a visual medium allows me to communicate ideas and feelings that I would find difficult to express in words. I use a wide variety of traditional and non-traditional materials as symbolic elements in my work. While the pieces are not always pretty, my goal is that they …
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Engineering T Cell Receptors for Potential Therapeutic Applications
… goal of developing new engineering strategies to allow for the isolation of high affinity mutants of many different TCRs was extended to methods for affinity maturation in Chapter 4. Previous affinity-based selections have involved incubating yeast cells with fluorescently labeled recombinant …
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Comparison of high level design methodologies for algorithmic IPs : Bluespec and C-based synthesis
… Design Language (HDL) that allows designers to express intended microarchitecture through high-level constructs. C-based design tools directly generate hardware from algorithms expressed in C/C++. This research compares these two design methodologies in developing hardware for Reed-Solomon …
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Body Language: Pain in Victorian Literature
“Body Language: Pain in Victorian Literature” argues that Victorian authors use the readable sign system of the body and pain to emphasize their characters’ physical features to the reader. As characters physically manifest emotions or experience violence, their appearances change, and these …
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Overexpression of Human Aryl Hydrocarbon Receptor in <i> E.coli</i> Using Two Different Solubility Enhancing Tags
… such as TCDD are environment pollutants whose toxic effects are mediated via aryl hydrocarbon receptor (AhR) signaling pathway. AhR is a ligand sensitive transcription factor. The unbound AhR resides in cytoplasm as a complex containing p23, Hsp90 and XAP2. Upon ligand binding, AhR undergoes …
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Soundfield simulation : the prediction and validation of acoustical behavior with compute models
… the past, acoustical consultants could only try to convince the client/architect that with calculations and geometrical plots they could create an acoustically superb space. Now, by modeling the significant acoustical parameters of a design, we can preview a proposed acoustical solution and it is …
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HEMLOCK: HEterogeneous ModeL Of Computation Kernel for SystemC
… in making an SLDL heterogeneous is the facility to express different Models Of Computation (MOC). Currently, all SystemC models employ a Discrete-Event simulation kernel making it difficult to express most MOCs without specific designer guidelines. This often makes it unnatural to express …
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Empathetic Consultation Skills in Undergraduate Medical Education: A Qualitative Approach.
… inquiry and administration of treatment, to the interaction and communication that occurs in consultations. The requisite for care in medical practice has been widely documented, and the role of empathy is acknowledged and recognised in this process; however, it is often difficult to …
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A Narrative of Charitable Acknowledgment: Reframing Interpersonal Communication in Intimate Relationships
… within intimate relationships is more difficult to come by--a condition stemming in part from an emotivist ethical paradigm. MacIntyre (1984) describes "emotivism" as the current state of society where individual preference serves as the ethical decision making compass. Emerging from …
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Fire on the Tongue: A collection of Poems
… promised as the last refuge of those who wished to breathe deeply, speak intimately, and inspire intensely. Others had the power to move me, but I seemed incapable of moving others; my lines lacked clarity, my phrases caused confusion. In a mood of irritable desperation I wrote these lines:</p> …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… itself as a stable, high yielding mammalian expression system for the production of recombinant proteins – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other platforms, CHO cells continue to dominate as the most utilised system due to their scalability, …
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Enhancement of plant expression vectors using replication and silencing suppressor elements
… is gaining traction as a cost-effective platform to produce recombinant proteins. Further improvements can be made, however, to increase overall yield especially for difficult to express proteins. In this study virus-derived silencing suppressors and replication elements were used with the aim of …
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A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli
Expression of recombinant enzymes in bacteria – principally E. coli - is an important method to generate high yields of industrially valuable proteins in short manufacturing timescales. While an extensive understanding of recombinant protein expression at industrial scale in E. coli has been …
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Development of recombinant immunotoxins targeting overexpressed cell surface biomarkers trophoblast cell-surface antigen 2 and mesothelin for cervical cancer treatment
… incidence rates. The disease is strongly linked to persistent infection by high-risk strains of the human papillomavirus (HPV). Despite the availability of HPV vaccines and early detection methods such as Pap smears and HPV testing, many women remain undiagnosed or only receive treatment in …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… antibodies such as Trastuzumab have been used to treat breast cancer via the human epidermal growth factor 2 (HER2) receptor. Immunotoxins (ITs) composed of an antibody fragment fused to apoptosis-inducing protein toxins targeting cellsurface antigens have been used for therapy of refractory …
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The Anxiety of Expression : Word, Image and Sound in 9/11 Fiction
<p>Responses to the terrorist attacks of September 11, 2001 came in many forms - political, social, cultural, and military. The events of that day shaped the first decade of the 21st century, and continue to have enormous resonance worldwide. This research project examines a particular aspect of …
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Recognition of hepatitis B epitopes by T cell receptors and T cell receptor-like antibodies
… cells through a heterodimeric surface receptor known as the αβ T cell receptor (TCR). The TCR recognizes a peptide epitope presented by a product of the major histocompatibility complex (MHC). All nucleated cells express class I MHC products, typically in association with one of thousands of …