Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"Dicer"”.
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Enhancement of Dicer as a personalized anticancer strategy
… key enzymes in the miRNA biogenesis pathway like Dicer, TAR RNA-binding protein (TRBP) and Drosha. Herein, the enhancement of miRNA processing as a therapeutic strategy is explored. Developing small molecule or peptide based therapeutics that mimic TRBP, an endogenous activator of Dicer, to …
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Bone Characterization of Dicer Deficient Transgenic Mice Under Dmp1 Promoter
… functional, miRNAs are cleaved by the enzyme “Dicer”. This study aims to evaluate the femoral and calvarial bone phenotypes of transgenic mice that lack functional miRNAs in osteocytes. The calvarial bone of age and sex-matched Dicer-deficient mice under the Dmp1 promoter and their controls …
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DiCER: A Design Research Method Arising From a Large Organisation
… the last two research questions, a method called DiCER has been developed for using large groups of ordinary people (non-ethnographers) to make fieldwork observations. In this method, groups of people are given a small amount of training and provided with support materials that allow them to make …
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Context Dependent Regulation of Dicer Function During Oocyte-to-Embryo Transition
<p>Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, …
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Dicer loss induces an oncogenic epigenetic switch in mesenchymal stem cells
… specification of the somatic state, we deleted Dicer in a murine mesenchymal stem cell model. Upon exploring the consequences of Dicer deletion, we identify a specific let-7 regulated mid-embryonic program within the global de-repression of miRNA targets accompanying Dicer loss. We further …
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Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
RNA interference (RNAi) manages many aspects of eukaryotic gene expression through sequence-specific interactions with RNA. Short RNAs, 20-30 nucleotides in length, guide the various effector proteins of RNAi to silence fully or partially complementary targets. The sequencing of endogenously …
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Discovery and biochemical characterization of RNA interference in budding yeast
… is converted into small-RNA duplexes by the Dicer enzyme. These duplexes are then loaded into the effector protein Argonaute to guide the cleavage of target transcripts. RNAi and related RNA-silencing pathways are found in plants, animals, fungi, and protists, suggesting origins in an early …
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The functional microRNA landscape of mammalian development
… gene expression. Dependent largely on the enzyme Dicer for their generation from longer precursor transcripts, mammalian miRNAs direct posttranscriptional repression of mRNAs based on complementarity to sites in their 3' UTRs. To better understand how these regulators impact fundamental processes …
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The Role of MicroRNAs in Craniofacial Development
… processes. To test this hypothesis a conditional Dicer knockout mouse model was used. In this model, floxed Dicer alleles were knocked out in the first pharyngeal arch precursor tissues of the palate. Embryos lacking Dicer in their palatal precursors experienced craniofacial developmental defects …
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Hypoxia Mediated Downregulation of Mirna Biogenesis Leads to Increased Tumor Progression
… by downregulation of two key enzymes, Drosha and Dicer in cancer cells. Previous data from our laboratory had shown the clinical relevance of downregulated Dicer and Drosha in ovarian and other cancer types, but a clear mechanistic understanding is needed for future clinical intervention …
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Microrna-mediated regulation and the fragile X family of proteins
… Furthermore, P-FMRP does not associate with Dicer or Dicer containing complexes in co-immunoprecipitation experiments or in an in vitro capture assay using a P-FMRP peptide sequence bound to agarose beads. These data show that Dicer containing complexes bind FMRP at amino acids 496-503 and …
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Investigating The Roles of The P63 Isoforms In The Microrna Biogenesis Pathway
… <em>TAp63</em> transcriptionally regulates <em>Dicer</em> while <em>ΔNp63</em> transcriptionally regulates <em>DGCR8</em>. <em>TAp63<sup> -/-</sup></em> mice are highly tumor prone. These mice develop metastatic mammary adenocarcinomas, squamous cell carcinomas, and lung adenocarcinomas to …
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The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection
… described. Virus replication increased in Dicer and RNA helicase A (RHA) knockout cells, but not when the central regulator of interferon synthesis IRF3 was knocked out. High-throughput Solexa Illumina sequencing was used to detect small viral RNA (svRNA) in Sindbis virus (SINV) infected …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… DROSHA, a RNase III enzyme, in the nucleus and DICER, another RNase III enzyme, in the cytoplasm. endo-siRNAs are distinguished from miRNAs in that endo-siRNAs are processed from naturally occurring long dsRNAs and the biogenesis of endo-siRNAs is DROSHA-independent but DICER-dependent. To …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… oligonucleotides are either first cleaved by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and directs the protein complex to homologous RNA. Cleavage of target RNA occurs opposite bases 10-11 when counting from the 5’ end of the hybridized siRNA …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… histone modifications. In chapter 4, I construct Dicer constitutive and conditional KO ESCs in the background of the Dnmt1f l/f l ESCs using CRISPR-Cas9. I dissect the role of DNA methylation and of DICER dependent small RNAs on transcriptional changes of ESCs. Additionally, I find that DICER …
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The Mechanism of Double-Stranded RNA Response in Neurospora
… of double-stranded RNA (dsRNA) by the enzyme Dicer initiates the RNA interference (RNAi) pathway, resulting in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and animals. In addition, dsRNA can …
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Peripubertal Binge Ethanol, Age and Sex Modulate Microrna Expression in the Ventral and Dorsal Hippocampus of the Adolescent Rat
… single-stranded microRNAs involves Drosha and Dicer enzymatic processing of microRNA precursors. A mature microRNA imperfectly base pairs with messenger RNA (mRNA) target genes,which leads to mRNA cleavage or translational repression. Our lab has found that repeated binge EtOH exposure alters …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… of Ago by performing CLIP in cells that lack Dicer, an enzyme required for mature miRNA biogenesis. We surprisingly found that Ago bound a similar set of genes in the absence of Dicer, and this overlap in target genes was due partially to residual, unprocessed miRNAs in the Dicer KO cells. …
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