Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Deubiquitination"”.
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Locus-specific proteomics identifies novel repressors of Epstein-Barr virus reactivation
… lytic cycle. This suggested an active process of deubiquitination, and subsequent analysis of whole-cell proteomic changes in lytic B-cells identified massive upregulation of the human deubiquitinase USP17. USP17 upregulation was driven by EBV lytic proteins and overexpression of USP17 was …
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The Role of K63-Linked Ubiquitination Cycles In Akt Kinase Activation
… regulating Akt signaling activation through deubiquitination of Akt.</p> <p>In summary, this study delineated the important mechanism of cycles of ubiquitination and deubiquitination of Akt in regulating membrane translocation and activation of Akt, and TRAF6 and CYLD as central switches for …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show that RNF20 stability is dependent upon USP7 removing ubiquitin chains that would mark RNF20 for proteasomal degradation. This interaction was seen within multiple cell lines, with the catalytic activity of USP7 being …
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Quantifying ubiquitin dynamics
… mechanisms. Defects in ubiquitination or deubiquitination have been linked to cancer onset and progression, muscle dystrophies, and disorders in inflammation and immunity; these findings further highlight the critical processes regulated by Ub. Due to its high demand, cellular Ub levels …
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LNCFOXP3 CONTROLS CELL IDENTITY AND FUNCTION OF HUMAN REGULATORY T CELLS
… to FOXP3 protein stability by promoting its deubiquitination in concert with USP7. Our findings disclosed another layer of FOXP3 regulation and support the role of lncRNAs in the maintenance of cell identity.
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An investigation into the role of deubiquitinating enzymes in plant disease resistance
… rosettes and suggests the crucial involvement of deubiquitination in the floral transition. The cDNA sequence of the tobacco AtUBP12 ortholog NtUBP12 was determined and utilised for VIGS based NbUBP12 gene silencing studies during disease resistance signalling in N. benthamiana. Loss of function …
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Biochemical and Genetic Studies on CC2D1A, a new NF-κB Activator and a Regulator of Synaptic Functions
… regulator of IKK complex, NEMO. CYLD, a deubiquitination enzyme specific for Lysine-63 linked polyubiquitin chains, negatively regulates the activity of CC2D1A. These results suggest that CC2D1A activates NF-κB through the canonical IKK pathway. In an attempt to identify the physiological …
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Cyclin B1 Mediates The Effect of Uchl1 In Promoting Cell Cycle Progression In Uterine Papillary Serous Carcinoma
… prior to mitosis. UCHL1 silencing increased the deubiquitination of cyclin B1, suggesting that UCHL1 counteracts the ubiquitination of cyclin B1 by the anaphase-promoting complex. Accordingly, UCHL1 silencing slowed the progression of cells into mitosis. Taken together, our findings indicate that …
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Molecular Recognition At Dna Damage Sites
… that RAP80 is a substrate of BRCC36 and this deubiquitination process is essential to regulate the BRCA1-RAP80 chromatin localization. Additionally, to understand the specific chromatin environment that recruits the RAP80 complex to DNA damage sites, we devised a novel methodology to purify …
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Tumor Suppressor function of the deubiquitinating enzyme BAP1 and its substrate gamma-tubulin In regulation of cell cycle and genome stability
… entry. In addition, we found that the g-tubulin deubiquitination enzyme, BRCA1 Associated Protein 1 (BAP1), was downregulated in metastatic adenocarcinoma breast cell lines compared to non-cancerous human breast epithelial cells. Reduced expression of BAP1 in breast cancer cell lines was …
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Ubiquitin Mediated Regulation of NF-kappa B Signaling
… tumor suppressor gene (CYLD) encodes a 110 kDa deubiquitination enzyme that negatively regulates NF-κB signaling. Loss-of-function mutations in CYLD lead to the disease Familial Cylindromatosis, which is characterized by the formation of benign skin tumors that originate from the head and neck …
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The role of USP17 in lysosome trafficking and lysosomal function
Ubiquitination and deubiquitination are the important types of post-translational modifications that are involved in the regulation of most complex cellular processes. USP17, a member of the ubiquitin specific protease (USP) family of deubiquitinases, has been shown to be required for cell cycle …
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Quantitative Deubiquitinase (DUB) Interactomics to Dissect the Biology of Cancer Associated DUBs
… regulating numerous cellular events. Conversely, deubiquitination is the removal of ubiquitin/polyubiquitin chains from ubiquitinated proteins. Deubiquitination is carried out by the class of enzymes called deubiquitinases (DUBs). These DUBs have vital roles in the regulation of protein …
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A Role for PI31-Mediated Proteasome Regulation in Proteostasis and Neuronal Health
… have focused on substrate recognition, binding, deubiquitination, unfolding, and translocation. However, evidence shows that the 26S complex itself can be regulated by the abundance of available subunits, rates of assembly and disassembly, posttranslational modification, localization, and a …
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The roles of papain-like protease-related proteins in viral replication and host immunity
… at least through the PLP2 domain-mediated deubiquitination and deISGylation effects. The infection of nsp2TF/nsp2N knockout mutants significantly upregulated antiviral innate immune responses in vitro. Furthermore, manipulating the expression of nsp2TF/nsp2N could enhance innate and …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
… chains from their distal end, an exo-specific deubiquitination. However, free UCH37 cleaves polyubiquitin poorly and is believed to be autoinhibited by its C-terminal UCHL5-like domain, or ULD, which may also be responsible for its oligomerization in solution. This work examines the structural, …
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An investigation of the role of CreB deubiquitinating enzyme in the regulation of carbon metabolism in Aspergillus nidulans
… by CreD together with ubiquitin ligase HulA, and deubiquitination, mediated by the deubiquitinating enzyme CreB, are involved in the regulatory pathway in A. nidulans. However, the molecular mechanisms are still unknown. Previously, partial loss-of-function alleles of creA and creB indicated …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
… lysosome, degrading and recycling its contents. Deubiquitination is the removal of ubiquitin, catalyzed by deubiquitinating enzymes (DUBs). The human ESCRT machinery recruits two DUBs: AMSH (associated molecule with a Src homology 3 (SH3) domain of signal transducing adaptor molecule (STAM) or …
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Defining The Functions of Usp22 and Usp44 In Regulation of H2Bub1 Levels
… not well understood.</p> <p>USP22, part of the deubiquitination module (DUBm) in the SAGA complex, is a well-defined regulator of H2Bub1 levels. ATXN7, another crucial subunit of the SAGA DUBm, is involved in a neurodegenerative disease, spinocerebellar ataxia type 7 (SCA7), due to a …
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