Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"Deoxyribozymes"”.
-
Kinase deoxyribozymes
… with the substrates. Both ribozymes and deoxyribozymes have been identified to catalyze the phosphorylation of oligonucleotides. However, previous efforts to identify kinase deoxyribozymes to catalyze the phosphorylation of amino acid side chains were unsuccessful because the …
-
Deoxyribozymes to hydrolyze DNA phosphodiester bonds
… single-stranded DNA oligonucleotides are called deoxyribozymes. Up to now, there are no known deoxyribozymes in nature, and the only method to identify them is in vitro selection. Since the identification of the first deoxyribozyme in 1994, many deoxyribozymes have been found to catalyze various …
-
Nucleobase-modified deoxyribozymes for amide and peptide hydrolysis
… catalysis. In fact, artificial DNA enzymes (deoxyribozymes) have been identified in laboratories by in vitro selection. The identification of new enzymes favors the use of nucleic acids over proteins for several reasons. First, nucleic acids can be amplified by natural enzymes, whereas …
-
Deoxyribozymes That Prepare Branched and Linear RNA by Ligation
… in making the native 3'-5' RNA linkage. Two deoxyribozymes, 9DB1 and 7DE5, from the second strategy have very few sequence requirements at the ligation junction. These deoxyribozymes will be useful as biochemical tools to study catalytic nucleic acids.
-
Development and execution of selection methods for lysine-acylating deoxyribozymes
This Thesis was approved for publication on 2022-04-29 at 11:20.
-
Deoxyribozymes for peptide substrates: exploring the landscape of nucleophiles and electrophiles
… catalyze various bioorganic reactions are called deoxyribozymes. Deoxyribozymes do not occur naturally and are identified using a combinatorial chemistry technique called in vitro selection. Since the discovery of the first artificial deoxyribozyme that catalyzes cleavage of phosphodiester bonds …
-
In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… capable of catalytic function. DNA enzymes, or deoxyribozymes, are not known to exist in nature, but they have been identified in lab by in vitro selection for a variety of chemical reactions. De novo identification of nucleic acid enzymes is possible because RNA and DNA can be amplified using …
-
Search for new types of deoxyribozymes and development of human topoisomerases inhibitors on the basis of oligonucleotides
… on the search and confirmation of new types of deoxyribozymes that possess DNA self-cleaving activity. Certain guanine-rich oligonucleotides with potential to form G-quadruplexes were constructed and their self-cleaving activities are examined during our investigations. Our studies demonstrated …
-
Synthetic coenzymes for in vitro selection of DNA enzymes.
… active single-stranded DNA molecules (deoxyribozymes) have been isolated from combinatorial libraries (pools) of randomized oligonucleotides. These enzyme-like DNAs customarily utilize divalent metal ions as cofactors in their catalysis. The metal ion cofactors are important because …
-
DNA catalysts for amide bond cleavage and for lysine side chain modification
… making the chances of discovering new deoxyribozymes likely. If deoxyribozymes can be identified that perform useful chemical reactions for which a catalyst has not yet been discovered, the impacts could be extensive. Protease enzymes, which catalyze cleavage of proteins, are essential …
-
Deoxyribozyme-Mediated Synthesis of Branched Nucleic Acids and Application to Modulation of Ribozyme Catalysis
Deoxyribozymes are single-stranded DNA molecules that catalyze a variety of chemical reactions. Catalytic DNAs have not been found in nature but can be identified by in vitro selection from random sequences. In this thesis, deoxyribozymes that ligate two RNA substrates or attach DNA to RNA are …
-
DNA enzymes for tyrosine PEGylation and azido-adenylylation of peptide and protein substrates
… be able to function as enzymes. DNA enzymes, or deoxyribozymes, have not been found in nature, but in vitro selection has led to the identification of deoxyribozymes for a variety of reactions. De novo enzyme identification favors the use of nucleic acids over proteins for several reasons. First, …
-
Deoxyribozyme-Mediated Synthesis of Branched and Lariat RNAs and Their Application in Studying Group II Intron's Splicing Mechanisms
… can be used for in vitro selection to identify deoxyribozymes with new activities.
-
Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
… catalytic competence of DNA. Indeed, the first deoxyribozymes were reported in 1994. However, DNA enzymes are not found in nature and can only be identified from a pool of random sequences via an artificial selection process, referred to as the in vitro selection. DNA catalysts capable of …
-
DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of Dna-Based Biosensors
… for diagnostic applications. Catalytic DNA (deoxyribozymes) can catalyze many different chemical reactions such as RNA ligation, RNA cleavage, DNA phosphorylation, DNA ligation, DNA adenylation, nucleopeptide formation, Diels-Alder reaction, and porphyrin metalation. RNA ligation is catalyzed …
-
DNA enzymes for peptide-nucleic acid conjugation and for lysine methylation
… an essential role in many biological processes. Deoxyribozymes have been previously identified to join tyrosine-containing peptides to RNA by reaction of the tyrosine (Tyr) hydroxyl group with the a-phosphate of a 5′-triphosphorylated RNA oligonucleotide (5′-pppRNA). The selection approach for …
-
DNA catalysts as phosphatases and as phosphoserine lyases
… identification of artificial DNA catalysts (deoxyribozymes) for chemical reactions is a valuable endeavor with long-term implications. Protein post-translational modifications (PTMs) are highly important in biological processes involving cellular regulation. Additionally, PTMs serve as …
-
DNA-catalyzed covalent modification of amino acid side chains in tethered and free peptide substrates
… (deoxyribozyme) discovered in 1994. Since then, deoxyribozymes have been shown to catalyze a wide variety of chemical reactions, including phosphodiester bond cleavage, nucleic acid ligation, thymine dimer photoreversion, and the Diels-Alder reaction. However, the majority of …
-
In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
… Similar approaches can be applied to develop deoxyribozymes that can probe other metal ions and at various concentration ranges.
-
Increasing the thermostability of a light-producing deoxyribozyme
… establish a framework for designing thermostable deoxyribozymes with potential applications in biosensing and diagnostics under thermally challenging conditions. Key words: deoxyribozyme, Supernova, thermostability, DNA triplex, chemiluminescence
Page 1 of 2