Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 37 for “"Density Gradient Centrifugation"”.
-
Development of Gold Nanocluster-Based Biosensors
… are isolated via a simple method based on density gradient centrifugation. Three distinct bands including monomers, small aggregates (2-4 nanospheres), and large aggregates (>5 nanospheres) can be separated via density gradient centrifugation. The isolated gold nanoclusters greatly enhance …
-
Complementary DNA to sea urchin histone messenger RNA
… phenol extraction followed by repeated sucrose density gradient centrifugation. When incubated in an ascites cell free protein synthesizing system this preparation of RNA was shown to support the synthesis of sea urchin histones confirming that this fraction contained histone mRNA.
-
The relationship of peanut stunt virus to cucumber mosaic virus and aspermy viruses of tomato and chrysanthemum
… (CV-L) varies on selected hosts. In sucrose density-gradient centrifugation each virus has a sedimentation rate of approximately 100s. Purified preparations of PSV and CV-L are homogenous and stable while CMV and TAV-B aggregate and lose infectivity. In acrylamide gel electrophoresis, all the …
-
Maize dwarf mosaic virus: purification, some physical properties, and yield of virus from several corn hybrids
… clarification and differential and sucrose density-gradient centrifugation. Corn tissue was homogenized in 0.1 M sodium citrate plus 0.5% mercaptoethanol. The virus was resuspended after high speed centrifugation in 0.005 M sodium citrate, pH 7.0. Purified virus has an ultraviolet absorption …
-
Glycosyl Transferases in Chondroitin Sulfate Biosynthesis: Subcellular Location and Elongation Reaction
… to subcellular fractionation by differential centrifugation and sucrose density gradient centrifugation. The glucuronosyl transferase, the N-acetylgalactosaminyl transferase, and also the sulfotransferases co-fractionated with the Golgi marker enzyme, galactosyl transferase. The specific …
-
Nanoparticle Superlattice Processing: Monodispersed Building-Blocks & Single Crystal Films
… crystallites and oriented crystal films, using density gradient centrifugation and scratch-directed assembly, respectively. Density gradient centrifugation allows for the separation of NCT assemblies, comprising various-sized crystallites, into discrete monodisperse distributions. This technique …
-
Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
… of ribosomes with [³H]-UTP was shown by sucrose density gradient centrifugation to have a sedimentation coefficient of 4S; the ratio of non-terminal to terminal incorporation of UTP was 6.08. The product synthesized after incubation of intact cells in the presence of [³H]-uridine and actinomycin …
-
Study of macroglobulinaemia in Trypanosoma equiperdum infections of the rabbit
… After separation of the 75 and 195 fractions by density gradient centrifugation, both fractions were found by complement fixation to contain anti trypanosome antibodies. These antibodies were also detected by immunofluorescence in the serum and 75 fractions, and by agglutination in the 195 …
-
Fatty acid synthase is a major polypeptide constituent of cytosolic lipoprotein and is associated with components of the milk lipid secretory pathway
… cytosol by gel exclusion chromatography or by density gradient centrifugation. The major polypeptide constituent of this lipoprotein aggregate was the monomer of fatty acid synthase (FAS). The major milk lipid globule proteins, butyrophilin (8u) and xanthine oxidase (XO), as well as the small …
-
Properties and Applications of Organic/Biodegradable Sorbents (Peat) For Remediation of Hazardous Waste Substances
… at Argonne National Lab, where a unique density-gradient centrifugation technique had previously been used to separate coal samples into their maceral components. After much experimentation and significant modification of these techniques, a successful separation of peat components was …
-
Photosystem 2 of anacystis nidulans
… pressure apparatus, follo.-Jed by differential centrifugation. 'Ihe ability to produce oxygen-evolving thylakoid membranes was determined by two factors, (a) the use of freshly harvested whole cells grown to late logarithmic phase and (b) the presence of glycerol and calcium in the cell-breakage …
-
Structural effects of neutral lipids on the divalent cation-induced interactions of phosphatidylserine - containing bilayers
… of X-ray diffraction, thin-layer chromatography, density gradient centrifugation and freeze-fracture electron microscopy, used in conjunction with an osmotic stress technique, showed that (i) -30 mole % PC can be accomodated in the Ca(DOPS)2 phase; and (ii) higher PC levels modify Ca2+-induced …
-
Lysine and methionine transport by bovine jejunal and ileal brush border membrane vesicles
… followed by differential and sucrose density gradient centrifugation. Membrane marker enzymes were used to determine the effectiveness of the fractionation procedure. Alkaline phosphatase and sodium-potassium adenosine triphosphatase served as the marker enzymes for the brush border …
-
Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
… as well as by RhoA and Rho-kinase. After sucrose density gradient centrifugation of HEK-293 cell lysates, we isolated two separate PIP2 pools. PLD1 and Arf1 selectively control the non-caveolar PIP2 pool in the high density fraction, whereas PLD2 affected PIP2 in both pools. In summary, these data …
-
Cell State Identication by Mass, Density, and volume
… tools, we developed four methods to measure cell density, the ratio of mass to volume. We first measure the average density of cell populations using the SMR and a Coulter counter. We observe that cell density increases prior to bud formation at the G1/S transition of budding yeast, which is …
-
Characterization and localization of a cyclic AMP dependant protein kinase from Dictyostelium discoideum
… able to dissociate the subunits when analyzed by density gradient centrifugation. Histone VII-S activated the subunits in the absence of cAMP but did not produce their dissociation. In contrast to the gel filtration data, sedimentation values indicated a dimeric holoenzyme structure. Reassociation …
-
APPROACHES TO IDENTIFY SURFACE PROTEINS OF ANAPLASMA PHAGOCYTOPHILUM DENSE-CORED ORGANISMS AS ADHESINS TO HUMAN P-SELECTIN GLYCOPROTEIN LIGAND-1
… biotinylation. In the process we developed new density-gradient centrifugation approaches which successfully purified an RC-enriched population as well as a mixed population of RC and DC organisms. Results from this work demonstrate that A. phagocytophilum DC organisms are responsible for …
-
Single-molecule studies of alpha-synuclein and abeta in Parkinson’s disease derived biofluids
… protein mixture by size using a sucrose density gradient centrifugation in chapter 5. We showed that the small aggregates below 200 nm are the toxic species in two biological assays by quantifying the extent of membrane disruption and consequent Ca2+ -influx into single-liposomes and the …
-
Antigen Trafficking within <em>Chlamydia trachomatis</em>-Infected Polarized Human Endometrial Epithelial Cells.
… membrane vesicles within host cells involved density gradient centrifugation for the separation of epithelial secretory pathway components followed by SDS-PAGE and Western blot to determine whether the chlamydial antigen-containing vesicles could fuse with and deliver the antigens to host cell …
-
Generation time statistics of Escherichia coli using synchronous culture techniques
… of E. coli are produced by the Mitchison-Vincent density gradient centrifugation technique. Very precise data are yielded by cell number counts at frequent intervals with a Coulter electronic particle counter. Culture growth is followed for three and sometimes four generations. Doubling times …
Page 1 of 2