Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"Demethylase"”.
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Crystal Structure and Mechanism of Lysine Specific Demethylase-1
… LSD1, the first known lysine-specific demethylase, selectively removes monomethyl and dimethyl, but not trimethyl modifications of Lys4 or Lys9 of histone-3. Here, we present the crystal structure of LSD1 at 2.9-A resolution. LSD1 forms a highly asymmetric, closely packed domain …
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Tet1: A Unique Dna Demethylase For Maintenance of Dna Methylation Pattern
… shown great potential as the long-sought DNA demethylases that induce DNA demethylation through multiple potential mechanisms. Here, we show that overexpression of TET1 catalytic domain alone (TET1-CD) but not full length TET1 (TET1-FL) induces global DNA demethylation in HEK293T cells. …
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Histone Lysine Demethylase KDM4A as a Therapeutic Target for Small Cell Lung Cancer
Small cell lung cancer (SCLC) arises from neuroendocrine cells within the lung. Along with deletion or loss-of-function RB1 and TP53 mutations, which occur in almost all cases, subsets of SCLC are driven by specific lineage transcription factors including ASCL1, NEUROD1, POU2F3, or YAP1. The …
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Investigating the Role of the Lysine-Specific Demethylase 4C in Pancreatic Ductal Adenocarcinoma
… pancreatic ductal adenocarcinoma (PDAC). Lysine demethylase 4C (KDM4C) is one of the chromatin modifying proteins frequently overexpressed across multiple solid cancers and is linked to chromatin instability, increased cell proliferation, and enhanced stem cell-like behavior. We observed …
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Stochastic Activation of Enhancers in the Innate Immune Response by the Histone Demethylase JMJD2D
… responses. siRNA-depletion of the H3K9 demethylase JMJD2d attenuates gene activation and overexpression of JMJD2d potentiates the IFN response. We find that the underlying mechanism involves the activation of enhancers – knockdown of JMJD2d attenuates stimulus-induced enhancer …
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Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation
… in the oocyte. KDM1B is a histone lysine demethylase whose activity is directed to H3K4me1 and H3K4me2, particularly within the gene body of actively transcribed genes. By generating CRISPR-Cas9-mediated knockout TSCs ablated for Kdm1b, I show that Esrrb is consistently down-regulated but …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
… stem cells (GSCs). Mutations in Lsd1, a histone demethylase exhibit GSC-like tumor formation. Clonal analysis, cell-type specific knock down and rescue experiments demonstrate that Lsd1 functions within the escort cells that reside immediately adjacent to cap cells (niche). Loss of Lsd1 causes …
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ROLE OF THE HISTONE DEMETHYLASE LSD1 IN THE REGULATION OF DIFFERENTIATION OF ACUTE PROMYELOCYTIC LEUKEMIA CELLS
… interact with LSD1 in other systems. LSD1 is a demethylase involved in transcriptional regulation, mainly acting on dimethylated lysine 4 of Histone H3 (H3K4me2). We showed that LSD1 inhibition sensitizes an APL cell line to physiological RA concentration. Here we characterize for the first time …
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JMJC Domain-Containing Histone Demethylase 2 (Jhd2): Bridging the Gap between H3K4 Trimethylation and H3 Acetylation
… of lysine residues. The enzyme Jhd2 is a histone demethylase that functions to remove H3K4 methylation in S. cerevisiae. Jhd2 is a homologue of the human JARID1 family of histone demethylases, which has four members: JARID1A, B, C and D. JARID1B is of particular interest because it has been shown …
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JMJC Domain-Containing Histone Demethylase 2 (Jhd2): Bridging the Gap between H3K4 Trimethylation and H3 Acetylation
… of lysine residues. The enzyme Jhd2 is a histone demethylase that functions to remove H3K4 methylation in S. cerevisiae. Jhd2 is a homologue of the human JARID1 family of histone demethylases, which has four members: JARID1A, B, C and D. JARID1B is of particular interest because it has been shown …
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Combined Inhibition of Lysine-Specific Demethylase 1 and Kinase Signaling as a Preclinical Treatment Strategy in Glioblastoma
… resulting in a poor prognosis. Lysine-specific demethylase 1 (LSD1) is overexpressed in glioblastoma and contributes to tumor growth and treatment resistance. Inhibitors of LSD1 have shown preclinical promise but have had limited clinical development for glioblastoma. Given the frequent kinase …
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Comparative efficacy of azole compounds targeting sterol 14-demethylase against multiple isolates of Acanthamoeba castellanii and polyphaga
… that target the cytochrome P450 enzyme sterol 14-demethylase, this enzyme is essential in the ergosterol pathway and its inhibition results in the loss of membrane stability and cell death. In this study five azoles; Econazole, Miconazole, Sulconazole, Tioconazole and Voriconazole where tested for …
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Lysine Specific Demethylase-1 and the Brahma Chromatin Remodeling Complex Regulate Conserved Signaling Pathways During Drosophila Wing Development
… deficiencies. I identified histone lysine demethylase enzymes as potential co-regulators of the Brahma complex and found that these demethylases were important for normal wing pattern development. Specifically, I identified Lysine Specific Demethylase-1 (LSD1) as a potential co-regulator of …
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ALTERNATIVE SPLICING AND PHOSPHORYLATION PROVIDE A MECHANISTIC BASIS FOR FUNCTIONAL SPECIALIZATION OF LSD1/KDM1 HISTONE DEMETHYLASE IN THE CENTRAL NERVOUS SYSTEM
… of four mammal-specific variants of the histone demethylase LSD1 / KDM1 arising from combinatorial retention of two alternatively spliced exons, resulting in either ubiquitous isoforms or neuro-restricted ones, which are dynamically regulated during cortical development. The neuro-specific …
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Efforts to Elucidate the Binding Interaction between Lysine-Specific Demethylase 1 (LSD1/KDM1) and CoREST and Their Roles in Breast Cancer
… until the recent discovery of histone demethylases. Lysine specific demethylase 1 (LSD1) is a unique histone demethylase that removes methyl groups on lysine residues and mediates expression of many genes important in cancer progression. LSD1 activity and its substrate specificity are …
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Analyzing The Interactions Of Kdm5/lid And Sin3 In Drosophila Melanogaster
… both a histone deactylase RPD3 and a histone demethylase dKDM5/LID. Immunopurification of dKDM5/LID verifies a previously observed interaction with SIN3 and RPD3. Furthermore, deficiency of dKDM5/LID phenocopies deficiency of SIN3 in many cellular and developmental processes. Knockdown of both …
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Pharmacological Inhibition of LSD1/KDM1A in Diffuse Midline Glioma Models and Impact on Kinase Signaling
… strategy. Epigenetic modulator, lysine specific demethylase 1 (LSD1) is a histone demethylase. It’s function demethylates mono- and di-methyl marks on H3K4 and H3K9 residues. This demethylase activity can impact gene transcription and overall gene expression, where dysregulated LSD1 expression …
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Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… dioxygenase gene family, namely thebaine 6-O-demethylase (T6ODM) and codeine O-demethylase (CODM). When assayed with a wider range of BIAs, CODM, T6ODM, and the functionally unassigned paralogs DIOX2 and DIOX7, renamed protopine O-dealkylase (PODA) and papaverine 7-O-demethylase (P7ODM), …
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Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus
… resistance, including mutations in the sterol-demethylase- encoding gene cyp51A, overexpression of sterol-demethylase genes, and overexpression of the efflux pump-encoding gene abcC, among a large collection of highly triazole- resistant clinical A. fumigatus isolates. Upon revealing that these …
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