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Showing 1 to 20 of 20 for “"Deiminase"”.
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Transglutaminase and peptidylarginine deiminase in the pathogenesis of autoimmune diseases
… the citrullinating enzyme, peptidylarginine deiminase (PAD). Previously, we have suggested that a complex between an enzyme and its modified substrate constitutes the neoantigen in autoimmune diseases. However, this hypothesis is challenged by findings in cases of primary Sjögren’s syndrome …
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Kinetic, Mechanistic, and Inhibition Studies On the Deiminase Family of Enzymes
<p>Protein arginine deiminase (PAD) and agmatine deiminase (AgD) both belong to the guanidino-group modifying enzymes superfamily. Although these two enzymes catalyze a similar reaction, the conversion of a guanidinium to a ureido and ammonia, their substrates differ greatly, i.e., …
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Kinetic Characterization of the Protein Arginine Deiminase Family: Mechanistic and Inhibition Studies
<p>The family of Protein Arginine Deiminases (PADs) include five mammalian isozymes (PAD1, 2, 3, 4, and 6) that catalyze the post-traslational modification of peptidyl-arginine to peptidyl-citrulline in numerous protein substrates. This modification results in numerous physiological and …
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Gene Knockout Of PRFA And The Arginine Deiminase Pathway In Listeria Monocytogenes
… involved in the disease process. The arginine deiminase pathway has the ability to regulate acidic environments by converting arginine to ammonia, ornithine, and ATP. With the help of a membrane-bound antiporter, the ornithine is transported out of the cell while the ammonia is converted to …
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Unlocking thePadlock: Elucidation of Protein Arginine Deiminase 4 Function, Activity and Activation Dynamics
The Protein Arginine Deiminase (PAD) family contains five known mammalian isozoymes (PADs 1, 2, 3, 4, and 6) that catalyze the post-translational modification of peptidyl-arginine to form peptidyl-citrulline in a variety of protein substrates. Over the past decade the importance of this post …
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Characterising the Role of the Calcium-dependent Citrullinating Enzyme Peptidyl Arginine Deiminase 2 in Ovarian Cancer
… of calcium-dependent peptidyl arginine deiminase 2 (PADI2) plays a key role in the tumorigenesis of several cancers; however, the role of PADI2 in the pathogenesis of EOC is yet to be investigated. Using RNA-seq and microarray data from primary serous ovarian cancers (The Cancer Genome …
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An investigation of Porphyromonas gingivalis peptidylarginine deiminase: a putative virulence factor in an animal model of inflammation.
… chronic periodontitis expresses peptidylarginine deiminase (PAD), an enzyme that converts peptide-bound arginine to citrulline. A relationship between human PADs and chronic inflammatory diseases has been proposed. Citrullinated a-enolase is a candidate auto-antigen in rheumatoid arthritis. …
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Vitamin D Inhibits Expression of Protein Arginine Deiminase 2 and 4 in Experimental Autoimmune Encephalomoyelitis Model Of Multiple Sclerosis
Multiple sclerosis (MS) is a disabling disease that afflicts an estimated two million people worldwide. The disease is characterized by degradation of the myelin sheath that insulates neurons of the central nervous system manifesting as a heterogeneous collection of symptoms. Two enzymes, protein …
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Arginase from Bacillus caldovelox
… media and screened for arginase, arginine deiminase and arginine decarboxylase activity by a reversed-phase liquid chromatography method developed to detect these arginine-catabolizing enzymes in cell-free extracts. Thermus, Bacillus and Sulfolobus strains produced arginase, but arginine …
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Discovery and characterization of ribosomally synthesized and post-translationally modified peptide natural products
… rare. Surprisingly, the peptidyl arginine deiminase responsible for the modification of arginine to citrulline in bacteria is not known. Comparative genomics was employed to find a putative PAD enzyme, which was subsequently heterologously expressed and tested for arginine deiminase …
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Inhibition of extracellular vesicle release enhances cancer chemotherapy and inhibition of outer membrane vesicle release sensitises bacteria to antibiotic and bacteriophage therapy
… effect on the cell viability. Peptidyl Arginine Deiminase (PAD) inhibitor, Chloramidine (Cl-amidine, Cl-am) and Protein Kinase C inhibitor, Bisindolylmaleimide-I (Bis-I) exhibited extensive EV inhibition in PC3 and MCF-7 cells. This effect was further tested using an Annexin-V-based apoptosis …
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The role of PADs in the biogenesis of prostate cancer microvesicles which play a dysfunctional role on DC biology and the therapeutic potential of skeletal muscle microvesicles on prostate cancer cells
… first time a novel function of peptidylarginine deiminase (PAD) isozymes (elevated in cancer cells) in the biogenesis of MVs. It was reported here that during the stimulation of cancer cells to microvesiculate, PAD expression and deimination of cytoskeletal-actins is increased. Inhibiting the …
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Synthesis of functionalised sulfonamides
… dimethylamino hydrolase (DDAH) and arginine deiminase (ADI). Several compounds displayed \muM inhibition against DDAH and ADI; and in addition, these currently represent the first known inhibitors of ADI. During the course of our investigation, it was also revealed that a small assortment of …
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Development of Novel Small-Molecule Therapeutics for Multiple Sclerosis
Protein arginine deiminase (PAD) activity is upregulated in MS and the overactive enzymes convert peptidyl arginine on myelin structural proteins to neutral citrulline variants. Hypercitrullination disrupts key ionic interactions between myelin proteins and lipid bilayers that are necessary to …
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Characterisation of HP1γ in mammalian cells
… residues are citrullinated by peptidyl arginine deiminase 4 (PADI4) in vitro and in vivo. Mutations in HP1γ (R38/9A), designed to mimic the loss of charge accompanied with citrullination, affect HP1γ’s binding to H3K9me3 peptides and reduce its residence time on chromatin in differentiated mESCs, …
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Autoimmune Consequences of Histone Deimination during Neutrophil Activation
… converted to citrullines by peptidylarginine deiminase IV (PAD4) creating structures that capture or "trap" bacterial pathogens. Neutrophil extracellular traps (NETs), as these structures are known, generate an extracellular complex of deiminated histones and bacterial cell adjuvants. The …
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Identificación taxonómica y clonal de bacterias acéticas, y estudio del efecto de la nisina frente a biofilms de bacterias enológicas
… that this LAB utilizes the ADI (arginine deiminase) pathway of arginine degradation as mechanism of response to the presence of ethanol. It generated ornithine and ammonium, but it did not generate any biogenic amine (agmatine, spermidine, phenylethylamine, histamine, or putrescine) either …
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Characterization of the Factors Associated with SCCmec Mobility in Staphylococcus Aureus
… IV and a genomic island containing an arginine deiminase pathway, known as ACME, inserted adjacent to one another in the SCCmec chromosomal attachment site. Each element was site-specifically excised from the chromosome by CcrAB, resulting in two independent, extra-chromosomal, circularized …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… experiments identified the arginine deiminase responsible for the installation of this functional groups. Solution NMR analysis generated a 3D solution NMR ensemble structure for this peptide. In Chapter 6 we report the discovery of two novel thiopeptide RiPPs. Genome-mining …
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Proteomics: A close encounter with rheumatology
… waarbij er een verhoging van peptidyl arginine deiminase (PAD) en gecirullineerd gliaal fibrillair zuur eiwit (GFAP) aangetoond werd. Het is gekend dat de getroffen gewrichten van RA patiënten hypoxisch zijn. Wegens de hyperplasie van het synoviaal weefsel wordt de afstand tussen de cellen en …