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Showing 1 to 20 of 102 for “"Dehydrogenases."”.
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Coenzyme engineering of NAD(P)+ dependent dehydrogenases
… the coenzyme selectivity of the NADP-dependent dehydrogenases and evolve mutated enzymes that accept biomimetic coenzymes. This is a major focus of this dissertation. Establishment of efficient screening methods to identify beneficial mutants from an enzymatic library is the most challenging …
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The Dynamics of Dehydrogenases - A Phase Space Odyssey
… a transition path sampling study of two lactate dehydrogenases, those of Plasmodium falciparum and Cryptosporidium parvum, which evolved through separate gene duplication events from a common malate dehydrogenase ancestor. It was found that though both lactate dehydrogenases share the same …
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Influence of Cellular Localization on Activity of Hydroxysteroid Dehydrogenases
Hydroxysteroid dehydrogenases (HSDs) catalyze the interconversion of inactive steroids and active hormones. HSDs use nicotinamide cofactors in the cytosol and endoplasmic reticulum (ER) lumen to either reduce or oxidize their steroid substrates. Our lab has extensively studied the 17beta-HSDs types …
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Targeting Aldehyde Dehydrogenases in NF2-null Meningioma and Schwannoma
Meningioma and schwannoma are tumours of the brain and nervous system that are frequently driven by loss or mutation of the gene NF2, which encodes the tumour suppressor protein Merlin. Dysregulated Hippo pathway signalling, caused by NF2 loss, drives overexpression of aldehyde dehydrogenase 1A1 …
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Lipoic acid assembly on 2-oxoacid dehydrogenases in E.coli
… cofactor essential for the activity of 2-oxoacid dehydrogenases and the glycine cleavage system. In the absence of lipoic acid modification the dehydrogenases are inactive and aerobic metabolism is blocked. In Escherichia coli two pathways for the attachment of lipoic acid exist, a de novo …
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Purification and Characterization of Two Dehydrogenases Acting on 5-Ketocamphor
Made available in DSpace on 2014-12-05T19:37:47Z (GMT). No. of bitstreams: 1 6104364.pdf: 2014053 bytes, checksum: f942be8c0e340c8e479ddd6bc00cf7fc (MD5) Previous issue date: 1961
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Steroid metabolism in the human gut by microbial hydroxysteroid dehydrogenases
… of steroid hydroxyl groups by hydroxysteroid dehydrogenases (HSDHs). Pairs of HSDHs can reversibly epimerize α-hydroxyl groups through an oxo-intermediate to β-hydroxyl groups. These small modifications of steroid structure often greatly influence their physicochemical properties, leading to …
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Directed evolution of amino acid dehydrogenases for biocatalysis of chiral amines
… this strategy to the engineering of amino acid dehydrogenases for biocatalysis of chiral amines, focusing on two crucial features for successful directed evolution experiments. A first key aspect is the development of technologies allowing the screening of large libraries of enzyme variants to …
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Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum
Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. …
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Cloning, Heterologous Expression, and Characterization of Human and Escherichia Coli Choline Dehydrogenases
Membrane bound choline dehydrogenase (CHDH) catalyzes the first committed step of choline oxidation to betaine-glycine in mammals and in some bacteria such as E. coli and P. aeruginosa . In mammals, it is an important source of one-carbon units for cytosolic folate- and AdoMet-dependent reactions, …
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A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli
… catalytically active protein. Commercial alcohol dehydrogenases are enzymes often produced in recombinant systems that are primarily employed in industrial chemical catalysis; the formation of inclusion bodies currently limits product yield of these enzymes. The primary aim of the work described …
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The nature of sorbital (a primary) and sorbose (a secondary) dehydrogenases of Gluconobacter species
… using the NAD(P)-independent membrane-bound dehydrogenases in which the products are released back to the medium. Reports of further limited oxidations of these primary oxidation products by <i>Gluconobacter</i> in single step or sequential oxidations by secondary dehydrogenases are also …
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Characterization of alternative NADH dehydrogenases in the respiratory chain of Toxoplasma gondii as a novel drug targets
Das singuläre Mitochondrium der schnell replizierenden Tachyzoiten von Toxoplasma gondii generiert ein Membranpotential, über dessen Beitrag zum Energiestoffwechsel des Bradyzoiten- oder Ruhestadiums des Parasiten bisher wenig bekannt ist.Die steady state mRNA-Konzentration von 11 nukleär-kodierten …
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Development of enzyme-catalysed resolution, desymmetrisation and cascade processes. Use of lipasas, alcohol dehydrogenases and transaminases in stereoslective synthesis
… Dynamic Kinetic Resolution Employing Alcohol Dehydrogenases” (Adv. Synth. Catal. 2019, DOI: 10.1002/adsc.201900317). En el Capítulo 5 se ha puesto a punto un método de desimetrización de 1,4-dicetonas utilizando la alcohol deshidrogenasa proveniente de Ralstonia species. Tras optimizar …
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Biochemical Characterization of <i>Toxoplasma gondii</i> Type II NADH Dehydrogenases - Physiological Impact on Mitochondrial Functions and Energy Metabolism
The single subunit mitochondrial type II NADH dehydrogenases (NDH2s) in apicomplexan parasites have been considered as potential drug targets due to the absence in mammalian cells. Toxoplasma gondii encodes two isoforms of NDH2s, TgNDH2-I and TgNDH2-II, whose functions are not known. Therefore, …
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Structural insights into microbial one-carbon metabolic enzymes: Ni–Fe–S-dependent carbon monoxide dehydrogenases and acetyl-CoA synthases
Carbon monoxide dehydrogenase (CODH) and acetyl-CoA synthase (ACS) enzymes play crucial rules in the global carbon cycle by catalyzing reversible carbon dioxide reduction and reversible acetyl-CoA synthesis, respectively. In some cases, CODHs are monofunctional, whereas in other cases CODHs form …
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Investigating the expression and function of aldehyde dehydrogenases in prostate cancer. Probing the expression and function of ALDHs using chemical probes, drugs and siRNA
… progression and drug resistance. Aldehyde dehydrogenases (ALDHs) have been used in some tumour types as CSC markers. Their high expression and high functional activity found in CSCs is also associated with drug resistance. Emerging evidence suggests deregulation of certain ALDH isoforms …
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