Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Dehalogenase"”.
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Substrate interactions during the anaerobic biodegradation of 1,1,1-trichloroethane
… concentration of a reduced metalloenzyme (the dehalogenase). The concentration of the reduced dehalogenase is controlled by the external concentrations of the electron donor and acceptor. Although Monod kinetics adequately described the relationship between TCA concentration and its …
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Isolation and Ecology of Bacterial Populations Involved in Reductive Dechlorination of Chlorinated Solvents
… 16S rRNA gene based analyses, and reductive dehalogenase gene targeted PCR approaches revealed that complete reductive dechlorination is carried out by multiple dechlorinators. Two new dechlorinating species were isolated from contaminated and non-contaminated site materials. The first new …
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Molekulargenetische und biochemische Untersuchungen zum Reaktionsmechanismus Cofaktor-freier 2,4-Dioxygenasen
… der bekannten Sekundärstruktur der Haloalkan Dehalogenase aus Xanthobacter autotrophicus lassen vermuten, dass die Enzyme Qdo und Hod strukturell mit der Enzymfamilie der Alpha/Beta-Hydrolasen verwandt sind. Ortsspezifische Mutagenese und biochemische Untersuchungen zeigten, das H244 sehr …
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Synthesis of cis- and trans-3-fluoroacrylate, and inhibition of BCL6 targeting a non-reactive cysteine
… be tested with cis- and trans-3-haloacrylate dehalogenase that has been shown to process the corresponding chloro- and bromo- isomers in an isomer-specific manner. These compounds might be processed like the other halo-acrylates (where the halo group if chloro or bromo) or might function as …
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Extending the utility of enzymes for site-specific targeting of fluorescent probes
… can efficiently recruit a modified haloalkane dehalogenase. These methods were used to label cells with diverse fluorophores, including quantum dots, and allowed tracking of single membrane proteins to study their lateral diffusion.
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Degradation of atrazine utilizing triazine hydrolase (TrzN) from Arthrobacter aurescens TC1
… aurescens TC1 is a Zn(II) dependent hydrolytic dehalogenase from the amidohydrolase super family. TrzN has 22 unique substrates. Most notably, TrzN can hydrolytically dechlorinate atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) to its less toxic derivative hydroxyatrazine. Atrazine …
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Uncovering the Role of 5'-Nucleotidases in Pyrimidine Metabolism in Trypanosoma brucei
… to the histidine-aspartate (HD) and haloacid dehalogenase (HAD) protein families. I found that HD 5'-nucleotidase (5'-HDNT) preferentially converts pyrimidine mononucleotides to nucleosides and was essential for parasite growth, while neither of the HAD enzymes was essential. Parasite …
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Bioindicators of Reductive Dechlorination in a Dehalococcoides ethenogenes-Containing Mixed Culture: Transcriptional Trends With Respect to Substrate Type, Substrate Concentration, and Culture Operation
… and an ATP synthase, and also multiple reductive dehalogenase (RDase), hydrogenase (H2ase) and other oxidoreductase enzymes were selected for study as potential bioindicators of reductive dechlorination in DET. Quantitative reverse-transcriptase PCR (qRT-PCR) data on selected gene transcripts in …
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SGK3 activity and regulation in PI3K-Akt pathway inhibitor resistance in breast cancer
… This compound utilizes the bacterial dehalogenase activity of HaloTag7 to specifically induce degradation of any endogenously Halo-tagged protein. I demonstrated that HaloPROTAC-E was able to induce degradation of the entire VPS34 complex to impact on downstream biology. This compound …
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Phosphate nutrition in the Ricinus communis L. seedling
… which are typical for a member of haloacid dehalogenase and DDDD superfamilies of enzymes catalyzing a diverse number of hydrolytic and phosphotransferase reactions. The functional analysis after expression of RcPS1 in E.coli showed significant acid phosphatase activity. The high transcript …
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Respuestas fúngicas a señales ambientales: Funciones de las proteínas CarO, OpsA, CutA y AcyA en Fusarium fujikuroi
… crassa, encoding a protein of the haloacid dehalogenase family with an unknown function in the osmotic stress response. Here we report on the functional analysis of cutA, the cut-1 ortholog of F. fujikuroi. cutA mRNA levels increased transiently after exposure to 0.68 M NaCl and were reduced …