Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"Dehalococcoides"”.
-
Spatial Relationship between the Trichloroethylene Degrading Bacteria Dehalococcoides Sp., Sulfate Reducers and Archaea during Reductive Dechlorination
… Archaea), sulfate reducing bacteria (SRB) and Dehalococcoides sp. (DHC) are of particular interest in this study. Three different types of bench scale reactors were constructed to model different aquifer types such as sandy, silty and fractured bedrock aquifers (hereafter type 1, 2 or 3 …
-
Bioindicators of Reductive Dechlorination in a Dehalococcoides ethenogenes-Containing Mixed Culture: Transcriptional Trends With Respect to Substrate Type, Substrate Concentration, and Culture Operation
… important gene transcripts were examined in a Dehalococcoides ethenogenes strain 195 (DET)-containing mixed culture (D2) capable of reductively dechlorinating tetrachloroethene (PCE) successively to trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), and non-toxic ethene …
-
Polybromattujen difenyylieettereiden (PBDE) anaerobinen brominpoisto sedimentissä
… elektronisieppausdetektorilla (GC-µECD). Dehalococcoides- ja yleisbakteerimäärät 16S rRNA -geenikopioina määritettiin qPCR:llä. Hypoteesit olivat, että brominpoisto on nopeampaa ennestään saastuneissa sedimenteissä, lämpimässä ja niissä mikrokosmoksissa, joissa on enemmän …
-
Redutive Dehalogenation of Chlorinated Alkanes by Novel Bacteria at the PetroProcessor of Louisiana Inc. Superfund Site
… Their closest previously cultured relatives are “Dehalococcoides” strains with 16S rRNA gene sequence similarities of only 90%. A quantitative real-time PCR (qPCR) approach targeting 16S rRNA genes indicated that both strains couple reductive dechlorination to cell growth. Growth was not observed …
-
Microbial Growth On Anthropogenic Compounds By Reductive Dehalogenation Or Oxidation
… because of their toxicity and persistent nature. Dehalococcoides mccartyi strain 195 can reductively dehalogenate some of these compounds and, in the case of tetrachloroethene (PCE), detoxify it by producing ethene. Its genome contains a putative prophage, which could inhibit its ability to grow …
-
Isolation and Ecology of Bacterial Populations Involved in Reductive Dechlorination of Chlorinated Solvents
The findings of this study demonstrate that Dehalococcoides species are intimately involved in complete reductive detoxification of chlorinated ethenes and are widely distributed in anoxic sediments and aquifers, including non-contaminated (pristine) environments. Careful examination of enrichment …
-
The effects of Fe(III) reduction on biodegradation of fuel oxygenates and chlorinated ethenes
… Quantitative PCR analysis revealed that Dehalococcoides and Geobacteraceae organisms were enriched concurrently in the dechlorinating Fe(III)-reducing sediments/cultures; Dehalococcoides abundance in the presence of Fe(III) was not significantly different from that in the cultures without …
-
Characterization of prokaryotic diversity in a chlorinated solvent-contaminated bedrock aquifer using denaturing gradient gel electrophoresis, ribosomal DNA sequencing, and real time PCR
… Geobacteraceae family, Dehalorespirers (Dehalococcoides sp. and Desulfuromonas sp.), and anaerobic methane oxidizers. Molecular fingerprints of the samples were generated by the use of denaturing gradient gel electrophoresis (DGGE), and were analyzed to detect spatial and seasonal …
-
Assessment of Microbial Biodegradation of Mixed Soil Contaminants at the Santa Susan Field Laboratory Using TRFLP, qPCR, and Culturing
… and PAHs, as well as the target gene for<em> Dehalococcoides </em>(an anaerobic dechlorinating bacteria)<em>. </em>For each culturing a model chemical was selected to represent each COI and added to Bushnell-Haas agar plates containing no added carbon source other than the model compounds. The …
-
Bioremediation of chlorinated ethanes and ethenes in vertical flow engineered wetland systems
… confirmed the presence of Dehalobacter sp. and Dehalococcoides sp. as well as the functional genes bvcA and tcrA reductase known to mediate the biodegradation of chlorinated ethanes and ethenes to non-toxic end products.
-
Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… and the medium for a slow-growing bacterium, Dehalococcoides ethenogenes 195, has been optimized. In addition, 13C-based metabolic flux analysis has been developed to quantitatively profile flux distributions in central metabolisms in a green sulfur bacterium, Chlorobaculum tepidum, and …
-
Microcosms and field bioremediation studies of Perchloroethene (PCE) contaminated soil and groundwater
… (commercially available culture containing Dehalococcoides). This culture is known to complete the degradation of PCE to a safe end product, ethene. Concentrations of PCE and its degradation intermediates were monitored in the gas phase of the microcosm vials. Biostimulation of the natural …
-
Distribution of Dehalococcoidia in marine sediments and strategies for their enrichment
… to cultivated Dehalococcoidia species such as Dehalococcoides mccartyi. Interestingly, among the tested organohalide compounds, 1,2,3-trichlorobenzene was transformed to 1,3-dichlorobenzene in sediment cultures. Inhibition studies using antibiotics against Gram-positives and microbial community …