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Showing 1 to 2 of 2 for “"Degenerate Oligonucleotide Primed PCR"”.

  1. Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment

    … based on whole genome amplification using degenerate oligonucleotide primed PCR (DOP-PCR). The approach can produce DNA libraries from nanograms of a template genome or the metagenome of multiple microbial populations. The DOP-PCR primers contain random bases, and thermodynamics of hairpin …

    vt Repository record for Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment (opens in a new tab)

  2. Genome complexity reduction for genome-wide single nucleotide polymorphism analysis

    … SNP genotyping techniques all rely on an initial PCR amplification of each SNP locus. Individual or low-level multiplexed PCR reactions are sufficient for genotyping a few to a few hundred different SNPs, but genome-wide linkage and association studies in humans will require thousands to tens of …

    mit Repository record for Genome complexity reduction for genome-wide single nucleotide polymorphism analysis (opens in a new tab)