Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"Deep Tissue Imaging"”.
-
Photoacoustic imaging: development of near infrared platforms for non-invasive deep-tissue imaging
"Fluorescence imaging has been employed as the gold-standard in molecular imaging. However, despite its ubiquitous usage, this technique is only viable for studying molecular interactions within a cellular system. Fluorescence imaging is limited by optical diffusion, at depths greater than 1 mm, …
-
Harmonic phase based low coherence interferometry : a method for studying the dynamics and structures of cells
… investigation as a means for optical deep tissue imaging.
-
Extending imaging depth of multiphoton microscopy
… fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread …
-
Nanoshells in vivo imaging using two-photon excitation microscopy
… for applications in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type 2 nanoshells can be excited with a NW laser in the micrometer range. Using NIR microscopy …
-
Computational microscopy for sample analysis
… explores the novel application of computational imaging methods to fluorescence microscopy and oblique illumination microscopy. In fluorescence spectroscopy, we have developed a novel nonlinear matrix unmixing algorithm to separate fluorescence spectra distorted by absorption effect. By extending …
-
Development of chemical tools for studying biology with light: Photoactivatable donors and photoacoustic probes
… activatable contrast agents for photoacoustic imaging, an emerging deep-tissue imaging modality. To address a lack of modular activatable probes for this imaging technique, we combined the analyte specificity of DNA aptamers with commercially available fluorophores to show the feasibility of a …
-
Development of high-speed two-photon microscopy for biological and medical applications
… powerful microscopic technologies for in-vivo 3D tissue imaging up to a few hundred micrometers. It has been finding important applications in neuronal imaging, tumor physiology study, and optical biopsy. A practical limitation of TPM is its slow imaging speed (0.3 1 frames/s). We designed …
-
Genetically encodable calcium sensors for Magnetic Resonance Imaging
… at the organismal level. Magnetic resonance imaging (MRI) provides a unique tool for bridging this gap, as it allows for imaging tissue throughout whole organisms. Although functional MRI (fMRI) is already a workhorse technique in human neuroscience research, current fMRI methods give us …
-
Large Two-photon Absorption of Highly Conjugated Porphyrin Arrays and Their in vivo Applications
… (TPM) has become a standard biological imaging tool due to its simplicity and versatility. The fundamental contrast mechanism is derived from fluorescence of intrinsic or extrinsic markers via simultaneous two-photon absorption which provides inherent optical sectioning capabilities. The …
-
Novel Bio-Imaging Techniques Based on Molecular Switching
Fluorescence microscopy has been a fundamental imaging tool for life science research. Fluorescence basically involves only two molecular states: the ground molecular state and the first singlet excited state (the fluorescent state). Astonishingly, it greatly diversified the applicability of …
-
Design and Development of Multiscale Microscopic Imaging Systems for Neuroscience Applications
Multiscale imaging systems enable the measurement of micro-scale activity from individual neurons and mesoscale activity from diverse brain regions for understanding functional properties and correlations of widely separated neurons. However, these techniques are often expensive, with complex …
-
Rare-Earth Nanoparticles for Non-invasive In Vivo Imaging of Immune Cells in Cancer Immunotherapy
… materials very attractive for high resolution imaging of deep tissue structures. They have narrow and tunable emission bands in regions of near-zero tissue autofluorescence (1300-1700nm), large anti- Stokes shift enabling clear separation of excitation and emission wavelengths, high …
-
Upconverting nanoparticles: pushing theory and technology towards biomedical applications
… significant challenge in developing non-invasive imaging and interrogation techniques with high spatial resolution and penetration depth for precise diagnosis and treatment. These techniques rely on non-ionising radiation and non-toxic contrast agents possessing excellent photo and chemical …
-
Localized Excitation Fluorescence Imaging (LEFI)
A major limitation in tissue engineering is the lack of nondestructive methods to assess the development of tissue scaffolds undergoing preconditioning in bioreactors. Due to significant optical scattering in most scaffolding materials, current microscope-based imaging methods cannot "see" through …
-
Multifunctional Hybrid Theranostic Nanoplatforms for Cancer Imaging and Therapy
… confinement, and complex designs for combining imaging and therapeutic agents. To address these issues, this dissertation explores the design, synthesis, characterization, and applications of multifunctional hybrid theranostic nanoplatforms, integrating imaging and therapeutic capabilities into …
-
Optical polarization control in free space and through random media using wavefront shaping
… material characterization, and biomedical imaging. The usefulness of light in these areas depends on the dexterity with which the state of the optical field, defined by the spatio-temporal distribution of the field's phase, intensity, polarization and coherence, can be controlled and …
-
On the origin of photons : understanding excitons and multiexcitons in colloidal semiconductor nanocrystals
… the use of infrared-emitting nanocrystals for deep-tissue imaging in biological research. Earlier work on the subject involved the use of emitters with significantly lower quantum yield, and we demonstrated a variety of applications which make use of the high quantum yield and chemical …
-
Deep structure ultrasound imaging using fundamental and third harmonic coded excitation techniques
… contrast-to-noise ratio (CNR) for an ultrasonic imaging system. The thickness mode of piezoelectric transducers produces a signal that is resonant at a fundamental frequency and odd harmonics. This behavior is exploited using coded excitation techniques to increase the usable bandwidth of the …
-
Synthesis and characterization of infrared quantum dots
… dots (QDs) in the infrared for biological imaging and optoelectronic devices. I concentrated primarily on controlling the size and size distribution of indium arsenide and cadmium arsenide QDs. In the nanocrystal community, classical nucleation and growth is often invoked to explain size …
-
The Contribution of Environmental Cues to Early Squamous Tumour Formation in the Upper Gastrointestinal Tract
… have revealed that normal epithelium in various tissue types harbour substantial populations of mutant clones similar to those found in cancer. This implies that the accumulation of somatic mutations alone may not be the sole cause of cancer and that other factors are required. Tumours are …