Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Deep Mutational Scanning (DMS)"”.
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Integrative approaches to decipher influenza evolution, antibody responses, and AI-driven specificity prediction
… through three interconnected research areas: (1) deep mutational scanning (DMS) to map sequence–function relationships in influenza viral proteins; (2) large-scale analysis of antibody responses to SARS-CoV-2 and influenza; and (3) development of AI models to predict antibody specificity. Chapter …
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Replaying Life's Tape With Intraclonal Germinal Center Evolution
… binding and expression of clone 2.1 through deep mutational scanning (DMS) and cryo-electron microscopy (CryoEM). DMS elucidated that chIgY B cells must avoid ~13 deleterious mutations in order to make one affinityincreasing mutations. CryoEM revealed that central paratope of clone 2.1 is …
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Synthetic Transcription Factor Allostery Mapping and Analysis
… engineered LacI-based anti-repressors. Through deep mutational scanning, we have generated comprehensive datasets correlating single-mutant genotypes with functional phenotypes. Building upon the experimental dataset and alongside ongoing machine learning efforts that utilize it, I introduce a …
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Probing PAX6-DNA interactions using high-throughput yeast one-hybrid assays and deep mutational scanning
… I used a combination of yeast one-hybrid and deep mutational scanning in competitive growth assays to probe the functional consequences of almost all the possible single amino acid variants in the paired domain of PAX6. The results showcase the capabilities of the assay to reproducibly …