Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Decellularization"”.
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Development of Cartilage-Derived Matrix Scaffolds via Crosslinking, Decellularization, and Ice-Templating
… cell-mediated contraction. Furthermore, the decellularization of CDM was investigated in order to remove possible sources of immunogenicity. This work found that both physical and chemical crosslinking techniques were capable of preventing cell-mediated contraction in CDM scaffolds; however, …
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STUDIES IN THE DEVELOPMENT OF A XENOGRAFT-DERIVED BONE SCAFFOLD
… bone of porcine (pig) femurs using a novel decellularization and chemical oxidation protocol. In the following chapters we discuss the development and characterization of this bone scaffold. The presented results show the decellularization protocol is able to efficiency remove porcine …
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Characterization and Implementation of a Decellularized Porcine Vessel as a Biologic Scaffold for a Blood Vessel Mimic
… the artery <em>in-vitro</em>. </p> <p> Decellularization is the process of removing all cellular components from a tissue, leaving an acellular structure of extracellular matrix. Understanding the clinical problem and the potential of the BVM, the aim of this thesis is to develop the …
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The Development of a Xenograft-based Scaffold for Tendon and Ligament Reconstruction
… thesis is based on the development of a novel decellularization and oxidation protocol. The data presented characterize both allogenic and xenogenic scaffolds using histological, mechanical and structural analyses. Scaffolds prepared using this protocol demonstrate decreased immunogenic …
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The Role of Biomechanics in Liver Tissue Engineering
… tissue engineering is to use the process of decellularization to create acellular liver scaffolds that retain the native tissue's extracellular matrix and architecture. These scaffolds can then be repopulated with a variety of hepatic and non-hepatic cell-types depending on the application, …
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DEVELOPMENT OF NOVEL BIOTECHNOLOGICAL APPROACHES FOR ACCELERATING GENETIC GAIN IN THE BOVINE
… This was followed by the optimization of a decellularization protocol to create a testicular biological scaffold that mimics the native tissue microenvironment, preserving its structural, biomechanical, and biochemical properties. This scaffold was designed to support in vitro meiosis. The …
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Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney
… as well as the subsequent in vitro cultivation. Decellularization is the process of removing all cells of an organ while preserving the extracellular matrix (ECM) in its native architecture and composition. The influence of different detergents and temperatures was analyzed, and the results …
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Applicazioni innovative dell’ingegneria biomedica
… recent strategies to obtain tissues in vitro, decellularization has developed. This method aims at producing acellular biological matrices to be used as scaffolds for new organs suitable for transplantation. The first part of PhD has dealt with the optimization of a decellularization protocol …
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The Decelluarized Human Umbilical Vein (HUV) as an Allogeneic Scaffold for Vocal Fold Tissue Engineering
… were conducted to determine the optimal decellularization protocol. Once this was determined, 15 cords were obtained to further examine the potential of the HUV as an acellular scaffold. Three cords from different donors (each sliced into 3 sections or scaffolds) were examined as follows: …
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The Decelluarized Human Umbilical Vein (HUV) as an Allogeneic Scaffold for Vocal Fold Tissue Engineering
… were conducted to determine the optimal decellularization protocol. Once this was determined, 15 cords were obtained to further examine the potential of the HUV as an acellular scaffold. Three cords from different donors (each sliced into 3 sections or scaffolds) were examined as follows: …
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Decellularized cartilage as a chondroinductive material for cartilage tissue engineering
… This work was successful in developing a decellularization method for hyaline cartilage fragments that removed 99% of cells, while retaining 87% of GAGs and also in determining a method to produce a homogenous nanopowder of DCC. Additionally, this research was the first to examine the …
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Investigative study of long-term packaging effects and donor characteristics on acellular dermis scaffolds
… manner for the medical community. While the decellularization techniques have been extensively researched, little is known about the effects of storage or the variability of donor characteristics on the decellularized ECM scaffold. In these studies, acellular dermal grafts stored in ethanol …
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Exploration of Decellularized Vegetal Scaffolds as New Biomaterials for Tissue Engineering
… sought. Recently, scaffolds resulting from the decellularization of vegetal tissue have been investigated to provide a biocompatible, easy to handle and sustainable alternative to current TE constructs. However, the exploration of these biomaterials is still in the nascent stage and the goal of …
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A Nanoindentation Device and the Scale-Dependent Mechanical Properties of Native and Decellularized Liver Tissue
… One technique in regenerative medicine is decellularization, the removal of the native cells from an organ leaving behind an intact structure of extracellular material to act as a scaffold for new cells. Characterizing the biomechanical properties of these scaffolds is important due to the …
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THE INFLUENCE OF THE MICROENVIRONMENT OF HEALTHY AND TUMORAL CELLS
… probes have been produced using a combination of decellularization procedures and Laser Microdissection (LMD) and tested against tumoral rat cell line. As reference, we also performed force spectroscopy experiments using AFM spherical probes functionalized with the two main ECM proteins, laminin …
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Development and Characterization of Tunable, Bioinspired Electrospun Constructs for Skeletal Muscle Engineering
… and sizes. After confirming the appropriate decellularization of skeletal muscle, the natural polymer was electrospun with varying degrees of fiber alignment and varying degrees of crosslinking. The impact of scaffold architecture and crosslinking density on fiber swelling, degradation …
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Development and Utilization of a Bioengineered 3D Human Lung Model to Study Pathogenesis of Lung Disease
… and assessment of the model. We have developed a decellularization procedure for native human lungs to produce whole human AC lung scaffolds that contain the desired anatomical structures and morphological structures that provide a 3D configuration and biological cues that facilitate production of …
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Development and characterization of advanced nitric oxide-delivering polymeric systems against cardiovascular complications
… No tripartite regional variations were observed. Decellularization did not reduce calcium in leaflets (p>0.05), but significantly removed wall and sinus calcium (p<0.05). However, 85% of phosphorus was removed from all valve tissues. Thus, the distribution of normal tissue calcium content is not …
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Optimizing a scaffoldless approach for cartilage tissue engineering
… application. Finally, the effects of various decellularization treatments were examined, and it was determined that it was possible to remove cells while maintaining construct functional properties. The results presented in this thesis are exciting, as they have allowed for a better …
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Effects of Liver Extracellular Matrix Gel Stiffness on Primary Hepatocyte Function
The extracellular matrix is a complex environment of mechanical and chemical cues vital to individual cell growth, tissue formation, and ultimately complete organ function. The field of regenerative medicine aims to recreate the unique biological properties found in specific tissue types and to …
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