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Showing 1 to 20 of 104 for “"Deaminase"”.
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Molecular and Kinetic Characteristics of wild type and mutant Porphobilinogen deaminase
… and function of the enzyme, porphobilinogen deaminase (PBGD), as well as experimentally demonstrating the use of kinetic, structural and thermodynamic approaches to shed light on the enzyme reaction. The key focus was to investigate the effect of three mutations of the active site lysine 98 …
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Molecular and Kinetic Characterisation of wild type and mutant Porphobilinogen Deaminase
… and function of the enzyme, porphobilinogen deaminase (PBGD), as well as experimentally demonstrating the use of kinetic, structural and thermodynamic approaches to shed light on the enzyme reaction. The key focus was to investigate the effect of three mutations of the active site lysine 98 …
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Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes
… through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific primers and …
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Characterizing the RNA Editing Specificityof ADAR Isoforms and Deaminase Domains In Vitro
<p>Adenosine deaminases acting on RNA (ADARs) convert adenosine-to-inosine in double stranded RNA. Selectivity of editing sites depends on the sequence of the RNA as well as the secondary structure. Identification of sites of ADAR editing by editome analysis is skewed due to the different abundance …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… immunodeficiency (SCID) due to adenosine deaminase (ADA) deficiency. Initial trials looked promising and the technique was extended to other forms of primary immunodeficiency. Unfortunately, some of the patients enrolled in these trials using retroviral vectors to carry replacement genes …
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Dual Targeting of Tumor Angiogenesis and Chemotherapy By Endostatin- Cytosine Deaminase-Uracil Phosphoribosyltransferase
… which linked endostatin (Endo) to cytosine deaminase-uracilvphosphoribosyl transferase (CD). Endo targets unique tumor endothelial cells toprovide tumor-specific antiangiogenesis activity and also carries CD to the localtumor area, where it serves nontoxic prodrug 5-fluorocytosine (5-FC) …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… is carried out by the homodimeric adenosine deaminase acting on tRNA (ADAT) and modifies only tRNAarg, whereas in eukarya the enzyme is the ADAT2/3 heterodimer, which performs the same reaction on all tRNA molecules with adenosine at position 34. Despite a similar evolutionary origin, the …
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AMP Deaminase 3 overexpression regulates cellular energetics and signaling for PGC-1α activation.
… explanation is increased enzyme activity of AMP Deaminase 3 (AMP [rightwards arrow] IMP + NH3), which is highly overexpressed in atrophic muscles and functions to prevent increases in [AMP]. We tested the hypothesis that AMPD3 overexpression would significantly attenuate DNP (mitochondria …
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Structural studies of the deaminase domain of the human HIV-1 restriction protein APOBEC3G
… role. A3G was identified as a single-strand DNA deaminase that potently inhibited the replication of the HIV-1ΔVif virus. It produces the nonfunctional provirus by deaminating the cytosines to uracils on the minus-strand viral cDNA. Consequently, A3G can genetically inactivate HIV and recent …
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The Role of Activation-Induced Cytidine Deaminase in Antibody Diversification and B Cell Malignancies
<p>Activation-induced cytidine deaminase (AID) is indispensable for somatic hypermutation (SHM) and class switch recombination (CSR) in B cells. SHM alters the affinity of an antibody for its corresponding antigen by introducing point mutations within the variable region of the immunoglobulin (Ig) …
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Die RNA-abhängige Adenosin-Deaminase ADAR1 : Defizienz führt zu hämatopoietischen Defekten und embryonalem Tod
… Bedeutung der RNA-abhängigen Adenosin-Deaminase ADAR1 durch Inaktivierung des ADAR1-Gens im Säugermodell Maus untersucht. Es konnte anhand von zwei ADAR1-defizienten Mutanten mit kongruentem Phänotyp gezeigt werden, dass ADAR1-Verlust zu embryonaler Letalität führt. Tiere mit einem …
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Investigation of the role of the DNA-modifying enzyme, activation-induced cytidine deaminase (AID), in cancer
… DNA-editing enzyme, Activation-Induced cytidine Deaminase (AID) is essential for antibody diversification and plays an important role in immunity. AID is specifically expressed in activated B-cells and mutates targeted DNA sites, diversifying the antibody repertoire. Due to its mutagenic nature, …
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The diagnostic value of adenosine deaminase activity in the ascitic fluid of patients with tuberculous peritonitis
… of patients, 3. Establish levels of adenosine deaminase activity which give the best discriminatory information in patients with ascites. 4. Determine what conditions may give rise to false positive or false negative results. 5. Finally, the study was designed to assess the relative diagnostic …
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The Effect of Activation Induced Cytidine Deaminase Phosphorylation and Herpes Virus Uracil Dna Glycosylase On Antibody Diversification
<p>Activation-induced cytidine deaminase (AID) is a mutagenic enzyme that is expressed in mammalian B-cells and initiates the antibody diversification processes of somatic hypermuntation (SHM) and isotype class switch recombination (CSR). AID is targeted to the immunoglobulin gene locus where it …
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Utility of chloride and adenosine deaminase measurement in cerebrosphinal fluid for the early presumptive diagnosis of tuberculous meningitis
Background: Chloride and adenosine deaminase measurements in cerebrospinal fluid are still sporadically requested as part of tuberculous meningitis work-up. In the literature, evidence is contradictory and opinion is divided on their utility in clinical practice. The accuracy of both for the early …
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