Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"Deamidation."”.
-
Deamidation of soy protein by protein-glutaminase: Process evaluation and effect of deamidation on protein functional properties and flavor-protein interactions
… the conformation of the proteins. While chemical deamidation of soy protein isolate was previously found to decrease flavor-protein binding, the use of an enzymatic method for deamidation is generally more desirable since it is substrate specific, can be conducted under mild reaction conditions, …
-
Studies of Protein Stability by Hydrogen Exchange-Mass Spectrometry
… the potential of using HX-MS in studying deamidation and aggregation of proteins which are considered as major degradation pathways of therapeutic proteins. In chapter 2 and 3, we assessed the ability of using HX-MS to predict protein deamidation and understand structural dynamics of mAbs …
-
The effect of degradation on the efficacy and utility of the biopharmaceutical enzyme Erwinia Chrysanthemi L-Asparaginase
… efficacy. Two factors potentially impacting ErA, deamidation and aggregation, were studied in detail. With respect to deamidation, an improvement on published methods for its detection in ErA was developed using a combination of capillary isoelectric focussing (cIEF) and strong chemical …
-
The isolation and characterisation of Transglutaminase 2 inhibitors from natural sources
… incorporation of polyamine into proteins and the deamidation of protein bound glutamine to glutamate. In coeliac disease the deamidating activity of TG2 in the intestinal lamina propria converts glutamine to glutamate in undigested gliadin peptides. This conversion elicits an immune response which …
-
Role of 4E-BP2 in neuronal translation
… undergoes brain-specific post-translational deamidation on asparagines N99 and N102, which are converted to aspartic acid. Asparagine deamidation is not catalysed by enzymes but can occur spontaneously and is induced by alkaline pH. Deamidated 4E-BP2 was shown to regulate the kinetics of …
-
High Resolution Mass Spectrometric Approaches To Study Protein Structure and Environment in Lyophilized Solids
… as chemical degradation (e.g. oxidation, deamidation, hydrolysis) and/or physical degradation (aggregation, phase separation). Since several degradation pathways are accelerated by diffusion in solution, proteins are lyophilized to improve stability. The lyophilized formulation may still …
-
Exploring SOD1 electron transfer, heterodimerization, and hetero-amyloid formation.
… it contains an intrinsic molecular clock, deamidation, that accumulates over time. Misfolding and aggregation of SOD1 have been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and, more recently, Parkinson's disease. To investigate the molecular factors …
-
Methods and applications in computational protein design
… susceptibility to degradation via aggregation, deamidation, and oxidation. We demonstrate this technique on a subset of degradation-prone amino acids in phosphotriesterase, an enzyme that hydrolyzes toxic organophosphates including pesticides and chemical warfare agents. Second, we introduce …
-
Folding, stability and aggregation of the long-lived eye lens protein human gamma D crystallin
… solubility. Covalent damage, including glutamine deamidation, of the lens crystallins increases with age and as a result of exposure to environmental insults. Such covalent damage may cause partial-unfolding into aggregation-prone confomations that cause cataract. The in vitro stability of …
-
Structural and Functional Characterization of B. Anthracis Udp-Glcnac 2-Epimerase and Salmonella Secreted Effector I
… spectrometry as a possible substrate undergoing deamidation at Gln61. The biological relevance of this result during infection is being investigated.</p>
-
Characterization of Enzymes in the L-Asparagine Metabolism Pathway in Legumes
… in tissues occurs through two major pathways: deamidation and transamination. The transamination pathway employs an unidentified α-ketosuccinamate (α-KSM ) reducing dehydrogenase (α-KSDH), as well as an ω-amidase which has not been fully characterized. Additionally, α-ketosuccinamate and …
-
Chemical and enzymatic modification of whey proteins
… in an intermediate moisture system. Enzymatic deamidation, via trypsin and chymotrypsin, resulted in proteins with improved solubility and enhanced in vitro digestibility. Enzymatic hydrolysis of whey proteins was performed using six different enzymes, namely, trypsin, chymotrypsin, papain, …
-
Outlining different perspectives for accurate MS-based proteomics in processed matrices - a case study on European feed control
… 300 covalent protein changes (e.g., oxidation, deamidation, carboxymethylation) were identified via classical (n = 53) and error-tolerant (n = 282) database searches. Analysis of ruminant-specific marker peptides and their modified variants indicate that protein quantification accuracy in …
-
Computational methods in palaeoproteomics: making the most of mass spectrometry data
… modules: MS data preparation, preprocessing, deamidation, automatic species identification, and postprocessing clustering methods. It uses publicly available data to illustrate these modules and discusses current limitations and the impact of different practices. The next chapter reports on a …
-
Escape to the interior: Characterization of the cytosolic delivery of C-terminal cargo subdomains by the N-terminus of pasteurella multocida toxin and subsequent toxin-mediated downregulation of Gaq-PLCb1 signaling through depletion of Gaq from the plasma membrane
… levels, although Ga11 has been shown to be a deamidation substrate for PMT. This strongly suggests that membrane depletion of Ga protein may be specific to certain Ga-proteins. The findings from these two studies reinforce that PMT-N serves as the cytosolic delivery vehicle for C-terminal …
-
Studies on the mode of action of dermonecrotic toxins: Effects on adipogenic signaling pathways and delivery of c-terminal cargo into the cytosol
The dermonecrotic toxin family consists of the dermonecrotic toxin from Bordetella spp. (DNT), the cytotoxic necrotizing factors from Escherichia coli (CNF1, CNF2 and CNF3) and Yersinia pseudotuberculosis (CNFY), and the mitogenic toxin from Pasteurella multocida (PMT). These relatively large …