Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Deadenylation"”.
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The function of PAB1 in translation and in PUF3 dependent deadenylation
… of the poly(A)-binding protein (PAB1) in deadenylation and translation is well known. How PAB1 inhibits deadenylation and promotes translation is not well understood. I have analyzed PAB1 variants, containing entire domain deletions and substitutions of yeast residues with human residues. …
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Functions of Deadenylation Factors In Mrna Decay and Mrna Processing Body Formation
… process of poly(A) tail shortening, also termed deadenylation, is important for post-transcriptional gene regulation, because deadenylation not only leads to mRNA translational inhibition but also is the first step of major mRNA degradation. Translationally inhibited mRNAs can be stored and/or …
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The Role of Phosphorylation In Pam2 Motif-Containing Proteins Mediated Messenger Rna Deadenylation
… cellular processes. However, its role in mRNA deadenylation, a process to remove poly adenosines from the mature mRNA 3’ end tail, is unclear. The length of poly(A) tail determines mRNA stability and translation efficiency. Poly(A)-binding protein (PABP), which binds to newly synthesized …
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THE ROLE OF SELECTIVE MRNA DEADENYLATION IN THE HOMEOSTATIC CONTROL OF INNATE IMMUNE SIGNALING
… and selective factors of the mRNA deadenylation machinery post-transcriptionally control interferon mRNAs both at steady state and post-stimulation. Loss of these mRNA decay factors leads to increased interferon levels and increased interferon signaling. Mechanistically, we find …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
<p>The mRNA deadenylation process influences multiple aspects of protein synthesis and is known to be the major factor controlling mRNA decay rates. My data demonstrates that yeast PAB1 plays both positive and negative roles in controlling deadenylation, and I have identified particular regions of …
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Functional analysis of the CAF1 protein
… CAF1-specific functional regions required for deadenylation in vivo, we targeted for mutagenesis six regions of CAF1 that are specifically conserved among CAF1 orthologs. Defects in three regions of the CAF1 protein (residues 173-175, residues 255-257 and residues 340-342, alleles caf1-1, …
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Mechanisms of mRNA substrate-selection by the Ccr4-Not deadenylase complex
… of the polyA tail from an mRNA, a process called deadenylation, is an important mechanism of gene expression regulation. It is the first step in the decay of the transcript, and is also linked to repression of translation. Deadenylation is predominantly catalysed by a conserved multi-protein …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… regulated. Poly(A) tail shortening, known as deadenylation, represses gene expression by initiating mRNA decay and inhibiting translation. Deadenylation is required for mRNA homeostasis; moreover, specific transcripts can be targeted for deadenylation, enabling gene expression to respond to …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
… of Sindbis virus transcripts is predominantly deadenylation-independent. As cellular mRNAs are often regulated by elements present in their 3’ untranslated regions (UTR), we hypothesized that these viral 3’UTR elements were functioning similarly to cellular mRNA stability elements resulting in …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… for a shift from distributive to processive deadenylation, but is not required for decapping or general decay during NMD. These results also revealed that this interaction plays a role in the normal shift in deadenylation mode for non-PTC containing mRNAs from the relatively slow mode of …
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Site-specific chemical and topological modifications to augment mRNA therapeutic potential
… We show that mocRNAs are subject to slower deadenylation and enhance therapeutic protein expression in cell lines and primary cell cultures. We expanded on this technology by creating mRNAs with chemically branched poly(A) tails, or multitail mRNAs, which increase the density of …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… with the primary role of CCR4 and CAF1 in the deadenylation and degradation of mRNA. Previous work in our lab has identified a physical connection between the CCR4-NOT complex and SPT5. The role of SPT5 in mRNA degradation was examined. Mutation in SPT5 or an spt4 deletion slowed the rate of …
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Characterization of the instability elements present within the 3' UTR of the glutaminase mRNA in WKPT kidney cells
… of tristetraprolin (TTP) greatly enhanced deadenylation and degradation of GemARE-A6o mRNA, but only slightly stimulated the decay of GemGA-A60 mRNA. The turnover rate of GemGA-A60 was greatly enhanced in a cytosolic extracts of a clonal line of WKPT cells that over express ζ-cryst by …
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Function and regulation of poly(A)-tail length
… results could be explained by differences in deadenylation rates, we performed a kinetic analysis in which we captured newly-made mRNAs of different age ranges. The deadenylation rates that we calculated after measuring tails over time correlated strongly with mRNA half-lives (Spearman R < …
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Global analysis of mRNA decay rates and RNA-binding specificity reveals novel roles for CUGBP1 and PARN deadenylase in muscle cells
… which interacts with CUGBP1 and mediates rapid deadenylation of TNF in HeLa cell extracts, also plays a role in mediating mRNA decay in muscle. We identified 64 mRNA targets whose decay was dependent on PARN. Moreover, deadenylation of the Brf2 mRNA was impaired in PARN knock-down cells …
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Mathematical Modeling of Circadian Gene Expression in Mammalian Cells
… rhythmic processes, which are transcription, deadenylation, polyadenylation, and degradation. We found that rhythmic deadenylation is the strongest contributor to the rhythmicity in poly(A) tail length and the rhythmicity in the abundance of the mRNA subpopulation with long poly(A) tails. In …
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Pumilio Regulates the Epithelial-Mesenchymal Transition and Gastrulation During Zebrafish Development
… cell maintenance, and neuronal growth through deadenylation, inhibition of translational elongation, and allow access of microRNAs to their targets. pumilio proteins have yet to be identified and functionally characterized in the zebrafish, Danio rerio. Here, three putative pumilio homologs in …
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ZFP36L3: a Unique Member of the Tristetraprolin Family of RNA-Binding Tandem Zinc Finger Proteins
… of certain cellular mRNAs, leading to their deadenylation and destabilization. Studies in knockout mice have demonstrated roles for three of the family members, TTP, ZFP36L1 (L1), and ZFP36L2 (L2), in inflammation, chorioallantoic fusion, and hematopoiesis, respectively. However, little is …
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Mammalian gene regulation through the 3' UTR
… in target mRNA translational repression or deadenylation, or both. Through analysis of mRNA expression data following miRNA or siRNA overexpression or inhibition, we uncovered novel targeting determinants that influence mRNA levels. These include the presence of distinct seed match types and …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… by mediating translational repression and deadenylation/decay of cognate mRNA. Dysregulation of miRNA expression have been associated with various types of cancer and developmental diseases. Typically, primary (pri-)miRNA transcripts are processed by Drosha complex into precursor …
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