Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"De-differentiation"”.
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In vitro culture of a chondrocyte-seeded peptide hydrogel and the effects of dynamic compression
… and lasting repair of articular cartilage include delivery of cells or cell-seeded scaffolds to a defect site to initiate de novo tissue regeneration. Biocompatible scaffolds assist in providing a template for cell distribution and extracellular matrix accumulation in a three-dimensional …
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Dissecting the function and targets of FOXG1 in glioblastoma
… former are thought to be responsible for tumour development and recurrence, and display self-renewal and differentiation capacity in vitro. Glioma stem cells (GSCs) are defined operationally by their capacity to initiate tumours on orthotopic transplant into immunocompromised mice. The Pollard …
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Stochastic processes in biological systems : selected problems
Majority of biological processes can not be described deterministically. Multple levels of regulation contribute to the noise in the observable properties of the cells: fluctuations are ubiquitous in biological networks and in their spatial organization. In this thesis we consider several examples …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… reprogramming and chromatin structure guide differentiation and define cell identity during embryogenesis. To maintain cell identity and genome integrity, epigenetic marks are faithfully inherited during DNA replication through tightly coordinated processes. Our recent work demonstrated …
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Identification and Classification of Novel Gene Products Involved in Development and Regeneration in Xenopus Laevis
A cornea-lens transdifferentiation-specific cDNA library has been used to characterize new molecular events in lens regeneration. Most of these genes are also expressed during lens development, illustrating the similarity between mechanisms of development and regeneration. A subset of genes; whose …
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The identification and characterisation of key genes involved in somatic embryogenesis in wheat (Triticum, aestivum L.)
… auxin, they possess the unique ability to de-differentiate and return to embryonic development, via a process known as `somatic embryogenesis' (SE). On removal of auxin these totipotent cells are then capable of re-differentiating and regenerating into full adult plants. Although well …
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Generation of induced progenitor-like (iPL) cells using interrupted reprogramming
… proliferation and/or incompletely restricted differentiation. We developed a novel "interrupted reprogramming" strategy without traversing the pluripotent state, to generate "induced Progenitor-Like (iPL) cells" using carefully timed transient expression of induced Pluripotent Stem (iPS) cell …
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Smooth Muscle Cell Stress Response: Essential in Atherosclerotic Pathogenesis
<p>Atherosclerosis remains the leading cause of death worldwide. Recent research using lineage tracing and single-cell RNA sequencing unveiled a previously undescribed and prominent role of smooth muscle cells (SMCs) in atherosclerotic plaque formation. SMCs undergo complex phenotypic modulation …
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A chemical biology study of human embryonic stem cell pluripotency and differentiation
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerative medicine. To fully realize its potentials, we not only need to further our understandings toward the molecular mechanisms governing pluripotency and differentiation, but also to find new tools to …
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Engineered hydrogels for tracking cell signaling from the outside-in
… (iPS). iPS cells have the same potential for differentiation as ES cells and hold immense promise for tissue engineering, regenerative medicine, and fundamental developmental biology research. However, the introduction of these reprogramming factors leads to epigenetic changes which are not …
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Generating a proteomic profile of neurogenesis, through the use of human foetal neural stem cells
Introduction Neurogenesis, the development of new neurons, starts soon after the formation of the neural tube and is largely completed by birth. Development of the brain after birth is mainly reliant on the formation of new connections between surviving neurons. However, adult neurogenesis does …
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Development of the Clinical Application of miRNA with Proregenerative Effect on the Heart
Rationale: Cardiovascular disorders are the first cause of mortality and morbidity worldwide. This is due, at least in part, to the poor regenerative capacity of the heart and the lack of drugs able to foster cardiac regeneration. Our recent work has identified a few human microRNAs (miRNAs), in …
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An investigation of insulin-like growth factor binding protein-5 (IGFBP-5) as a biomarker for the detection of early liver disease
… rates of both men and women increasing considerably over the last decade from liver disease, along with the number of patients on the active liver transplant list in the UK on the increase, new diagnostic tools would be welcomed by hepatologists. This trend is possibly mirroring the increase …
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Stem cells and neoplasia a study of acquired melanocytic naevi
… interplay of genetic and environmental factors. Despite recent advances, the aetiology and pathogenesis of melanocytic neoplasms remains unclear and the anatomical location and state of differentiation of the initiating cell remains to be elucidated. Traditional models propose melanoma arises …
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Peptide microarrays for the discovery of cell-ligand interactions that direct cell state
During development, cells receive many cues from their environment. These cues, whether physical or chemical in nature, are able to regulate the behavior of cells from macroscale levels such as tissue organization, to microscale levels such as gene modifications. Synthetic materials are able to …
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Melanocyte proliferation in wounded skin in vitro: a histological and immunohistochemical analysis
… of most cutaneous scars is the lack of adequate repigmentation of the neoepidermis. In order to effect successful wound healing by restoration of effective skin barrier function, keratinocytes must proliferate and migrate to cover the gap created by the wound in an efficient and timeous …
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Transcriptional Regulation by Hypoxia-Inducible Factors in Tumor Cells
… human morbidity and mortality, and the risk of developing cancer is about one in three life times. Neuroblastoma is the most common extra-cranial solid tumor among children and arises from early sympathetic nervous system (SNS) cells arrested in their development. Generally, a low tumor cell …
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LINEAGE TRACING ANALYSIS OF PANCREATIC ACINAR CELL DE DIFFERENTIATION AND TRANS-DIFFERENTIATION IN VITRO AND IN VIVO IN THE ABSENCE OF MIST 1
… factors regulating this process have not been defined. The acinar specific transcription factor MIST1 is required for acinar cell maturation and targeted deletion of Misti (Mist1'A) in mice results in incomplete maturation of acinar cells, and increased susceptibility to pancreatic injury. …