Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Database searching"”.
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Advance metabolite identification from tandem mass spectra using deep generative models
… features of the precursor molecules. The database-searching strategy is the most popular for metabolite identification among its peers. It matches the query MS/MS spectrum to a database of molecule candidates, identifying the metabolite that best matches the query spectrum. This study uses …
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Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution
… also enabled error tolerant and multiplexed database searching---all clearly demonstrated in this thesis.
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The impact of diversity training in organizations: a systematic review using a meta-analysis methodology from 1991-2006
… Articles were identified through computer database searching and hand searching of selected journals, from 1991 through 2006. Of the 166 studies reviewed at the second-level screening, only eight studies met the criteria for inclusion. Seven studies provided analyses on multiple outcomes. …
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Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
… by proteomic mass spectrometry (LC-MS) and database searching. Finally, the carbohydrate to lipid metabolic pathway of B. sp. cultivated under natural conditions was determined. The total carbohydrate content was found to be 23 % per milligram biomass dry weight with monomeric sugars …
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Knowledge synthesis in the biomedical literature: Nordihydroguaiaretic acid and breast cancer.
… knowledge synthesis from publicly accessible databases, based on the model of D.R. Swanson. Knowledge synthesis endeavors bring together two or more non-interactive literatures to create combinatorial research data on a specific topic. In this endeavor the biomedical literature was searched on …
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Improved Dynamic Range, Quantitation, and Characterization of Histone H4 Post-Translational Modifications: A Top Down Mass Spectrometric Approach
… of modifications on H4 led us to develop a novel database searching strategy to simplify data analysis. Histone H4 was ""shotgun annotated"", resulting in the population of a database with masses of hypothetical modified H4 forms. Querying this database with ECD spectra rich in fragment-ions, we …
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Mass Spectrometric Characterization of the MCF7 Cancer Cell Line: Proteome Profile and Cancer Biomarkers
… set-up, sample preparation, data acquisition and database searching parameters, and to enable the detection and confident identification of a large number of proteins. Over ~4,500 proteins were identified using conventional filtering parameters, and >2000 proteins using a combination of filters …
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Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis
… the proteome of multiple tissues in F. grandis. Database searching resulted in the identification of 253 non-redundant proteins in five tissues: liver, muscle, brain, gill, and heart. Identifications include enzymes of energy metabolism, heat shock proteins, and structural proteins. The protein …
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Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture
… identified via Mass Spectroscopy and database searching. It was observed that Calreticulin, Glyceraldehyde-3-phosphate dehydrogenase, and alpha-crystallin B chain were up regulated in protein expression while Moesin (C-terminal frag), ii Poly (rc)-binding protein, Alpha-tropomyosin, …
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Quantitative pharmacoproteomics investigation of anti-cancer drugs in mouse. Development and optimisation of proteomics workflows for evaluating the effect of anti-cancer drugs on mouse liver
… before analysis by mass spectrometry and database searching for protein identification. Set A and Set B resulted in identification and quantification of 1146 and 1743 proteins, respectively. Moreover, Set A and Set B recovered 26 and 34 cytochrome P450 isoforms, respectively. The …
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Optimized GeLC-MS/MS for Bottom-Up Proteomics
… borderline statistical confidence produced by database searching. These hits are typically produced by matching a few marginal quality MS/MS spectra to database peptide sequences and represent a significant bottleneck in proteomics. A method was developed for rapid validation of borderline …
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Homology-Based Functional Proteomics By Mass Spectrometry and Advanced Informatic Methods
… developments in biological sequence database searching with mass spectrometry data. Conventional methods to identify proteins by mass spectrometry analysis have employed proteolytic digestion, fragmentation of resultant peptides, and the correlation of acquired tandem mass spectra …
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Complex Proteoform Identification Using Top-Down Mass Spectrometry
… of candidate proteins from a large proteome database for a query mass spectrum. Second, we describe mass graph-based alignment algorithms that efficiently identify proteoforms with variable post-translational modifications and/or terminal truncations. Third, we propose a Markov chain Monte …
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Flavonoids and Related Compounds as Nucleoside Transporter Inhibitors
… criteria for inhibitor design, and for database searching to find new hit molecules. Additionally, fifteen compounds were selected based on SAR and screened for equilibrative nucleoside transporter inhibition for validation of QSAR models. One novel compound, XI was designed with reduced …
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Protein expression in Fusobacterium nucleatum ATCC 10953 biofilm cells induced by an alkaline environment.
… were identified using mass spectrometry and database searching. These proteins were classified according to functional class, including metabolic, transport and stress proteins. One of the most interesting findings was the significant up-regulation of Fusobacterial Outer Membrane Protein A …
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A proteomic approach to investigate the response of tef (Eragrostis tef) to drought
… to peptide OFFGEL fractionation and appropriate database searching with the Tef Extended and Liliopsida databases enabled the generation of three dataset results. These datasets, each contained a substantial amount of database matched proteins, where 5727 proteins for the Tef Extended (TE), 2656 …
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Pearl Harvesting Autism
… the difficulties of on-line information searching as it relates to the topic of autism. A review of existing search strategies is presented and their limitations are discussed. A new method for deriving a set of search-terms for comprehensive searching, Pearl Harvesting, was tested here …
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Proteomic Investigation of Endocrine Therapy Resistance in Breast Cancer Investigating the Molecular Mechanisms for SERM Resistant Cell Lines Using SILAC-Based Proteomic Approach
… (2D-LC MS/MS). Proteins were identified by database searching using MascotTM. Relative changes (resistant/WT ratio) in protein levels were determined and bioinformatics tools (STRING and UniProt) used to explore significantly changed pathways associated with resistance. Western blotting was …
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Metabolomic modelling and applications in inflammatory bowel diseases
… with authentic external standards, and database searching. The acquired data was analysed using both unsupervised (PCA-X) and supervised (OPLS-DA) models in SIMCA in order to determine discriminating metabolite biomarkers responsible for the observed clustering patterns.;Results: …
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The Cluster Hypothesis: A Visual/Statistical Analysis
… be possible to predict the success of clustered searching based on the lexical characteristics of users' natural-language expression of their information need.
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