Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Data-independent acquisition (DIA)"”.
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A flexible and versatile framework for statistical design and analysis of quantitative mass spectrometry-based proteomic experiments
… are computationally efficient for large datasets, and maximize the reproducibility of the results.</p> <p>We propose a family of linear mixed effects models, and a split-plot view of the experimental design, that represent measurements from quantitative mass spectrometry-based proteomics. …
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Investigating the ubiquitin conjugating machinery in *Plasmodium falciparum*
… and gametocytogenesis. The utilisation of data-independent acquisition (DIA) mass spectrometry generated the largest Plasmodium ubiquitome to date. These findings contribute to a deeper understanding of the ubiquitin pathway in *P.falciparum* and provide potential targets for the …
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Quantitative crosslinking mass spectrometry
… To assess the reproducibility of crosslinking data I first adapted the quantitation software Skyline, which required reformatting crosslinked peptides as linear peptides. Using bis[sulfosuccinimidyl] suberate (BS3)-crosslinked human serum albumin (HSA), I found QCLMS to have a similar …
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Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
… techniques and their utilization with commercial data processing software to improve results from IM-enhanced data-independent acquisition (HDMSᴱ) proteomics strategies. Chemometric deconvolution and post-IM/collision-induced dissociation (postIM/CID) MS were used to extract "pure component" IM …
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Deciphering Metabolic Adaptations in Salmonella Enterica Serovar Typhimurium 14028S During Batch Growth and Gentamicin Exposure Using Absolute Quantitative Proteomics
… the mechanisms underlying antibiotic-mediated bacterial cell death is crucial for the development of novel strategies to control bacterial infections. Although the primary targets of antibiotics are well known, the actual molecular events leading to bacterial growth inhibition and cell …
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Improving reproducibility and standards in quantitative N-Glycoproteomic data
… drives glycoproteomics to rely on observational data from tandem mass spectrometry; mass spectrometry is a powerful tool that comes with logistical and technical limitations on the availability and compatibility of data. N -Glycopeptides can be identified in tandem mass spectrometry data, but …
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Clarification of the xanthan biosynthesis mechanism in Xanthomonas campestris pv. campestris B100 by mass spectrometry-based proteome analysis
… were particularly interesting. The reference database indicates that 2416 proteins were detected throughout the study, accounting for 54.75% of the proteome. Fluctuations in nitrate levels seemed to impact the quantity of most proteins related to nitrogen metabolism, except for Gdh and GlnA. …
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Assessing the usage of alternative transcripts in human tissues from RNA-seq and proteomics data
… We can now use RNA sequencing (RNA-seq) data to identify which isoforms of each gene are being expressed in a specific condition, by quantifying the expression level of each isoform of a gene, even though quantification of isoforms remains a difficult task. In this way, we can better …