Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"DT40 cells"”.
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Disruption of UHRF1 expression in chicken DT40 cells
… expression in a wide range of different cancer cells, and its ability to facilitate the crosstalk between DNA methylation and histone modification, thereby driving the occurrence of cancer and ensuring the inheritance of accurate epigenomic information to descendent cells. Depletion of UHRF1 …
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An investigation into the regulation of replication factories and dormant origins in human cells
… excess dormant origins are required to help the cells overcome replicative stress caused by barriers to the replication fork. Cells also regulate replication factories in a Chk1-dependent manner in response to replicative stress. The combination of dormant origin activation and inhibition of …
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Towards the understanding of pericentriolar satellite biology
… microtubule-organizing centre in eukaryotic cells. In cycling cells the centrosome promotes spindle assembly and the faithful execution of mitosis. In non-cycling cells it is involved in forming the cilium, a plasma membrane-resident organelle, which mediates crucial signalling pathways in …
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Gene Targeting in a Novel Mouse Model and the Chicken DT40 Cell Line
… genes, followed by transplanting the corrected cells back into the patient. However, the natural rate of gene targeting is too low to be of practical use in most cells; an exception to this is the chicken DT40 cell line which has a high relative rate of gene targeting (gene targeting rate/ …
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The Non-Wnt Functions of APC: Unravelling the Link between APC and Apoptosis
… APC. To this end, I created degron-tagged APC in DT40 cells that allowed for the rapid, conditional degradation of endogenous APC. The aim was to identify the immediate effects on cellular processes. Then, to identify the contribution of different APC domains by measuring the ability to rescue any …
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The role of the BARD1 BRCT domains in the DNA damage response
… cancer. In order to maintain genome integrity, cells have several different mechanisms to repair various types of DNA damage. BRCA1 is a tumour suppressor gene commonly mutated in many inherited breast and ovarian cancers. BRCA1 is fundamental to the repair to DNA damage especially the faithful …
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Genetic selection of cyclic peptide G-quadruplex ligands
… binding properties and subsequently imaged in cells. SICLOPPS, the second methodology, allows genetic expression of head-tail cyclised peptides in living systems. SICLOPPS libraries allow generation and selection of ligands that lead to a biological response, as opposed to a purely biophysical …
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In vitro diversification of chimeric human/chicken antibodies
Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et al., 2002). The chicken derived DT40 B cell line has been adapted for the expression and diversification of humanized …